Reviewed, UniProtKB/Swiss-Prot Q1IWN0 (CARA_DEIGD)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	Dgeo_2060

Organism

Deinococcus geothermalis (strain DSM 11300) [Complete proteome] [HAMAP]

Taxonomic identifier

319795 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_1000138858
Regions
	Domain	192 – 379	188	Glutamine amidotransferase type-1
	Region	1 – 188	188	CPSase HAMAP MF_01209
Sites
	Active site	267	1	Nucleophile By similarity
	Active site	352	1	By similarity
	Active site	354	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IWN0-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 704683FDF8A7AB21	FASTA	394	43,391
	10 20 30 40 50 60 MIRKERAILA LEDGTVYRGY AFGHRGETVG EVVFNTSMTG YQEIMTDPSY NGQIVTITYP 70 80 90 100 110 120 HVGNYGVAIY DMESNKPYVR GFIAREFSGE YSNHRAQQSL EAFMQQYGVV SIQGIDTRAL 130 140 150 160 170 180 VRRLRTGGVV KGVIAHRSYT HPEDPYGEFT PAEEQIYVQR ARDHQDIDGH DMTKEVTTPL 190 200 210 220 230 240 PYAFPTLRHG KRVVLMDFGI KHTIIERLAE VGIEPIVVPA HTTPAQIMAL QPHGLFLSNG 250 260 270 280 290 300 PGDPAPLEYA HKTAWELMGL LPTFGICLGH QILGLAAGGQ TFKMKFGHRG GNQPVKNLLT 310 320 330 340 350 360 GNVEITSQNH GYAVDIASIP DGAFVATHVN LNDGTLEGMA HSRYPVFSVQ YHPEASPGPH 370 380 390 DSRYLFDRFI EEIDAFEGAN GSPVLKASAG RLGV

References

[1]

"Complete sequence of chromosome 1 of Deinococcus geothermalis DSM 11300." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Daly M.J., Fredrickson J.K., Makarova K.S., Gaidamakova E.K., Zhai M., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000359 Genomic DNA. Translation: ABF46354.1.
RefSeq	YP_605523.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IWN0.
Genome annotation databases
GeneID	4058406.
GenomeReviews	Gene locus Dgeo_2060 in contig CP000359_GR.
KEGG	dge:Dgeo_2060.
NMPDR	fig|68909.1.peg.2245.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1IWN0.
OMA	LFDGSNC.
Enzyme and pathway databases
BioCyc	DGEO319795:DGEO_2060-MON.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_DEIGD

Accession

Primary (citable) accession number: Q1IWN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 13, 2006
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)