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Q1IWK0 (TRMB_DEIGD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Dgeo_2090
OrganismDeinococcus geothermalis (strain DSM 11300) [Complete proteome] [HAMAP]
Taxonomic identifier319795 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288144

Sites

Active site1051 By similarity
Binding site291S-adenosyl-L-methionine By similarity
Binding site551S-adenosyl-L-methionine By similarity
Binding site1051S-adenosyl-L-methionine By similarity
Binding site1091Substrate By similarity
Binding site1411Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1IWK0 [UniParc].

Last modified June 13, 2006. Version 1.
Checksum: 79FECB6096E34734

FASTA33136,336
        10         20         30         40         50         60 
MIFRLADFHF PDSAARLYPD TPQRPWILEV GFGDGRFWPH YAATFPEAPN YLGVEISGVS 

        70         80         90        100        110        120 
LLKAERRLRE AGLSNAVLTK LPATPLIREV VPAGSLDAIV VNFPDPWPKA GHAEHRLLRA 

       130        140        150        160        170        180 
PFFRLAASRL KPGGAVLLTT DHDEYFEFAC REAEASGVMR AELTDPPPAA LETKYARKWR 

       190        200        210        220        230        240 
ELGLEVQHAR FVPTHHPHVP HGTVARFPDS EDAPAVPHAI LTLPAAFDPG AFHKHTARGG 

       250        260        270        280        290        300 
QTREDPVGWT VVLLELYRSL KQDGWVMLAH VVEGELTQEV LIGISARGDG SHLVRLASFG 

       310        320        330 
GPIITPGVKA AVGVVTDWLE EQGAAVRHRG Y 

« Hide

References

[1]"Complete sequence of chromosome 1 of Deinococcus geothermalis DSM 11300."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Daly M.J., Fredrickson J.K., Makarova K.S., Gaidamakova E.K., Zhai M., Richardson P.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 11300.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000359 Genomic DNA. Translation: ABF46384.1.
RefSeqYP_605553.1. NC_008025.1.

3D structure databases

ProteinModelPortalQ1IWK0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING319795.Dgeo_2090.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF46384; ABF46384; Dgeo_2090.
GeneID4058187.
KEGGdge:Dgeo_2090.
PATRIC21626153. VBIDeiGeo41128_2689.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000099573.
KOK03439.
OMATKYALKW.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK445190.

Enzyme and pathway databases

BioCycDGEO319795:GHMU-2137-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_DEIGD
AccessionPrimary (citable) accession number: Q1IWK0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 13, 2006
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways