Reviewed, UniProtKB/Swiss-Prot Q1IVP4 (SYE1_ACIBL)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Acid345_0051

Organism

Acidobacteria bacterium (strain Ellin345) [Complete proteome] [HAMAP]

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367599
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IVP4-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: F79F1C0E02478A89	FASTA	518	58,269
	10 20 30 40 50 60 MSDKKVRARF APSPTGQLHV GNARTALFNW LFARQQGGTM ILRIEDTDLE RSEARYEQQL 70 80 90 100 110 120 LDDLKWMGIN WDEGPDVGGP FPPYRQSDKI DVYREHAERL VAEGKAYYCF CSQADLDAYR 130 140 150 160 170 180 EQALKDHRPP IYPGTCRSVD PAEAKRRRDS GEAGAIRLRI PERPIRFHDI VHGDVEFSNE 190 200 210 220 230 240 VVSDPIILRS SGVPVYNYVV VVDDAEMQIT HVIRGDDHLS NTPKQVALYE ALGWAVPEFA 250 260 270 280 290 300 HLSTILGPDR ERLSKRHGAT SIATFREMGV LPEALVNYIA LLGWAPTGGT REIFRPEELV 310 320 330 340 350 360 KEFDLRRVTP SPAIFDFEKL YWLNRHYIKE AATYRIAKLA RHYWDRMMID KIAHRVGMPK 370 380 390 400 410 420 LDPIHVAQWG ASNEVDEWLS KVTALVVPSV NKLDELPERG KVFFDYDAAA ALANPDNAEV 430 440 450 460 470 480 LAAPKTTDVL AAFTTRIANE SAPLTAERFK AIVNEVKTET GVKGKDLFHP IRIAVTGTHS 490 500 510 GPEFDKLVPL IEEGASLNLP IAVKSVKERV AEFASARN

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000360 Genomic DNA. Translation: ABF39056.1.
RefSeq	YP_589130.1.
3D structure databases
SMR	Q1IVP4. Positions 6-493.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IVP4.
Genome annotation databases
GeneID	4069985.
GenomeReviews	Gene locus Acid345_0051 in contig CP000360_GR.
KEGG	aba:Acid345_0051.
NMPDR	fig|204669.6.peg.55.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	AEMQITH.
PhylomeDB	Q1IVP4.
Enzyme and pathway databases
BioCyc	ABAC204669:ACID345_0051-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_ACIBL

Accession

Primary (citable) accession number: Q1IVP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 13, 2006
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)