Q1IU64 (LEXA_KORVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LexA repressor
EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	Acid345_0581

Organism

Koribacter versatilis (strain Ellin345)

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	LexA repressor HAMAP MF_00015		PRO_1000001250
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	131	1	For autocatalytic cleavage activity By similarity
	Active site	169	1	For autocatalytic cleavage activity By similarity
	Site	98 – 99	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IU64 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 14C9029717DD6717	FASTA	210	23,047
	10 20 30 40 50 60 MVALTRRQRE MYDFLCSFTD SHGYSPSFEE IAEGMGLSSL ATVHKHIGNL ESKGLLKRDY 70 80 90 100 110 120 NRARSIEVLR PKGQLKKSMA AAAAVATAGL PFLGRIAAGQ PIEAIENPET ISLGDFTGSK 130 140 150 160 170 180 EVFVLQVSGE SMQDEHIVDG DYVLVERINT ARDGEIVVAL VENSDTTLKR IYREGETVRL 190 200 210 QPSNAKMQPI RVPAGSVQVQ GRVIGVLRKY

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ellin345.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000360 Genomic DNA. Translation: ABF39586.1.
RefSeq	YP_589660.1. NC_008009.1.
3D structure databases
ProteinModelPortal	Q1IU64.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IU64.
Protein family/group databases
MEROPS	S24.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4068940.
GenomeReviews	Gene locus Acid345_0581 in contig CP000360_GR.
KEGG	aba:Acid345_0581.
NMPDR	fig|204669.6.peg.572.
PATRIC	31977932. VBICanKor57425_0618.
Phylogenomic databases
eggNOG	COG1974.
HOGENOM	HBG679610.
OMA	IAENPDF.
PhylomeDB	Q1IU64.
ProtClustDB	PRK00215.
Enzyme and pathway databases
BioCyc	ABAC204669:ACID345_0581-MONOMER.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01356.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR00498. LexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_KORVE

Accession

Primary (citable) accession number: Q1IU64

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program