Reviewed, UniProtKB/Swiss-Prot Q1IS80 (PROA_ACIBL)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Acid345_1268

Organism

Acidobacteria bacterium (strain Ellin345) [Complete proteome] [HAMAP]

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Acidobacteriales › Acidobacteriaceae

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Gamma-glutamyl phosphate reductase		PRO_0000252561

Sequences

	Sequence		Length	Mass (Da)
	Q1IS80-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 7F51E3D1C2D9298A	FASTA	409	43,990
	10 20 30 40 50 60 MTTREKLEAA RRAAPVVAEL STESKNALLL ALARTIDERT EEILAANRAD LEASGLDGSL 70 80 90 100 110 120 RDRLLLTPER IATMAEGLRE VAALADPVGE TLAEWERPNG LRIRKVRVPL GVVAIIYEAR 130 140 150 160 170 180 PNVTIDVIGL ALKSGNAVVL RGGKEAVRSN ECLVKIAGAT PGMPDGAIQL LDASNRESVQ 190 200 210 220 230 240 QLMKARGLVD VIVPRGGAGL IQFVVENSTV PVIETGAGNC HIFVDESANL DMADRIVINA 250 260 270 280 290 300 KTQRPSVCNA AEKLLVHRAI AKEYVPRIVK LLLDHGVEVR GDAETLALAQ GMQVAEATSA 310 320 330 340 350 360 DWDEEYLRLC MAVKVVADVD EAIAHINQHS TKHSESIITA NDAHARRFLR AADSAAVYWN 370 380 390 400 ASTRFTDGAE FGFGAEMGIS TQKLHCRGPF ALAELTSSKY EVIGSGQVR

References

[1]

"Complete sequence of Acidobacteria bacterium Ellin345." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Kiss H., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Kuske C., Janssen P.H., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000360 Genomic DNA. Translation: ABF40270.1.
RefSeq	YP_590344.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4073238.
GenomeReviews	Gene locus Acid345_1268 in contig CP000360_GR.
KEGG	aba:Acid345_1268.
NMPDR	fig|204669.6.peg.1255.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1IS80.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ACIBL

Accession

Primary (citable) accession number: Q1IS80

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	June 13, 2006
Last modified:	November 25, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)