Reviewed, UniProtKB/Swiss-Prot Q1IS76 (RTCA_ACIBL)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	Acid345_1272

Organism

Acidobacteria bacterium (strain Ellin345) [Complete proteome] [HAMAP]

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000264792
Sites
	Active site	316	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IS76-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: CEB9354906316A89	FASTA	355	37,475
	10 20 30 40 50 60 MRCGPKGLNM IVIDGSQGEG GGQILRTALS LSMVTGQPFT IEKIRANRER GGLLRQHLTA 70 80 90 100 110 120 VLAAAEISGA NVTGAELGSR TLTFAPQRVT PGDYSFAVGT AGSATLVLQT LLPALMLANK 130 140 150 160 170 180 PSNVVVEGGT HNMAAPPFDF LAKTFAPIIE SMGPKLKLTL DRYGFYPAGG GKITAEIEPV 190 200 210 220 230 240 EKLKQLELLD RGEVLQQNAV ALLAHLPRHI ADRELETASN ILGLAPESTR ILGTKNSAGP 250 260 270 280 290 300 GNALMIEVQT ERHTEIFTAF GKVGISAEKV AEEAAVAAAQ YVASSAFACE HLADQLLLPI 310 320 330 340 350 ALAGGGSFTA VRQTMHARTN IEIIRLFLPV SFEISEEENC ARVALSTRDR AGVSA

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000360 Genomic DNA. Translation: ABF40274.1.
RefSeq	YP_590348.1.
3D structure databases
SMR	Q1IS76. Positions 10-345.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IS76.
Genome annotation databases
GeneID	4073242.
GenomeReviews	Gene locus Acid345_1272 in contig CP000360_GR.
KEGG	aba:Acid345_1272.
NMPDR	fig|204669.6.peg.1259.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0430.
HOGENOM	HBG683539.
OMA	EIDGSYG.
PhylomeDB	Q1IS76.
Enzyme and pathway databases
BioCyc	ABAC204669:ACID345_1272-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR020723. RNA3'_term_phos_cycl_prd. IPR017770. RNA3'_term_phos_cycl_sub. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_ACIBL

Accession

Primary (citable) accession number: Q1IS76

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	June 13, 2006
Last modified:	February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)