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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Koribacter versatilis (strain Ellin345)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091ATPUniRule annotation
Active sitei316 – 3161Tele-AMP-histidine intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi291 – 2955ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciKVER204669:GHL8-1283-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:Acid345_1272
OrganismiKoribacter versatilis (strain Ellin345)
Taxonomic identifieri204669 [NCBI]
Taxonomic lineageiBacteriaAcidobacteriaAcidobacterialesAcidobacteriaceaeCandidatus Koribacter
ProteomesiUP000002432 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355RNA 3'-terminal phosphate cyclasePRO_0000264792Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi204669.Acid345_1272.

Structurei

3D structure databases

ProteinModelPortaliQ1IS76.
SMRiQ1IS76. Positions 10-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK01974.
OMAiMGDQIIP.
OrthoDBiEOG6RNQDX.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1IS76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCGPKGLNM IVIDGSQGEG GGQILRTALS LSMVTGQPFT IEKIRANRER
60 70 80 90 100
GGLLRQHLTA VLAAAEISGA NVTGAELGSR TLTFAPQRVT PGDYSFAVGT
110 120 130 140 150
AGSATLVLQT LLPALMLANK PSNVVVEGGT HNMAAPPFDF LAKTFAPIIE
160 170 180 190 200
SMGPKLKLTL DRYGFYPAGG GKITAEIEPV EKLKQLELLD RGEVLQQNAV
210 220 230 240 250
ALLAHLPRHI ADRELETASN ILGLAPESTR ILGTKNSAGP GNALMIEVQT
260 270 280 290 300
ERHTEIFTAF GKVGISAEKV AEEAAVAAAQ YVASSAFACE HLADQLLLPI
310 320 330 340 350
ALAGGGSFTA VRQTMHARTN IEIIRLFLPV SFEISEEENC ARVALSTRDR

AGVSA
Length:355
Mass (Da):37,475
Last modified:June 13, 2006 - v1
Checksum:iCEB9354906316A89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000360 Genomic DNA. Translation: ABF40274.1.
RefSeqiYP_590348.1. NC_008009.1.

Genome annotation databases

EnsemblBacteriaiABF40274; ABF40274; Acid345_1272.
KEGGiaba:Acid345_1272.
PATRICi31979424. VBICanKor57425_1357.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000360 Genomic DNA. Translation: ABF40274.1.
RefSeqiYP_590348.1. NC_008009.1.

3D structure databases

ProteinModelPortaliQ1IS76.
SMRiQ1IS76. Positions 10-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204669.Acid345_1272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF40274; ABF40274; Acid345_1272.
KEGGiaba:Acid345_1272.
PATRICi31979424. VBICanKor57425_1357.

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
KOiK01974.
OMAiMGDQIIP.
OrthoDBiEOG6RNQDX.

Enzyme and pathway databases

BioCyciKVER204669:GHL8-1283-MONOMER.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Ellin345.

Entry informationi

Entry nameiRTCA_KORVE
AccessioniPrimary (citable) accession number: Q1IS76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 13, 2006
Last modified: April 1, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.