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Q1IS76 (RTCA_KORVE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase

Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4
Gene names
Name:rtcA
Ordered Locus Names:Acid345_1272
OrganismKoribacter versatilis (strain Ellin345) [Reference proteome] [HAMAP]
Taxonomic identifier204669 [NCBI]
Taxonomic lineageBacteriaAcidobacteriaCandidatus Koribacter

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00200.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-3'-phosphate cyclase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
PRO_0000264792

Regions

Nucleotide binding291 – 2955ATP By similarity

Sites

Active site3161Tele-AMP-histidine intermediate By similarity
Binding site1091ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1IS76 [UniParc].

Last modified June 13, 2006. Version 1.
Checksum: CEB9354906316A89

FASTA35537,475
        10         20         30         40         50         60 
MRCGPKGLNM IVIDGSQGEG GGQILRTALS LSMVTGQPFT IEKIRANRER GGLLRQHLTA 

        70         80         90        100        110        120 
VLAAAEISGA NVTGAELGSR TLTFAPQRVT PGDYSFAVGT AGSATLVLQT LLPALMLANK 

       130        140        150        160        170        180 
PSNVVVEGGT HNMAAPPFDF LAKTFAPIIE SMGPKLKLTL DRYGFYPAGG GKITAEIEPV 

       190        200        210        220        230        240 
EKLKQLELLD RGEVLQQNAV ALLAHLPRHI ADRELETASN ILGLAPESTR ILGTKNSAGP 

       250        260        270        280        290        300 
GNALMIEVQT ERHTEIFTAF GKVGISAEKV AEEAAVAAAQ YVASSAFACE HLADQLLLPI 

       310        320        330        340        350 
ALAGGGSFTA VRQTMHARTN IEIIRLFLPV SFEISEEENC ARVALSTRDR AGVSA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000360 Genomic DNA. Translation: ABF40274.1.
RefSeqYP_590348.1. NC_008009.1.

3D structure databases

ProteinModelPortalQ1IS76.
SMRQ1IS76. Positions 10-345.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204669.Acid345_1272.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF40274; ABF40274; Acid345_1272.
GeneID4073242.
KEGGaba:Acid345_1272.
PATRIC31979424. VBICanKor57425_1357.

Phylogenomic databases

eggNOGCOG0430.
HOGENOMHOG000015264.
KOK01974.
OMARRGHYPK.
OrthoDBEOG6RNQDX.

Enzyme and pathway databases

BioCycKVER204669:GHL8-1283-MONOMER.

Family and domain databases

Gene3D3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPMF_00200. RTC.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRTCA_KORVE
AccessionPrimary (citable) accession number: Q1IS76
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 13, 2006
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families