Reviewed, UniProtKB/Swiss-Prot Q1IPU7 (AMPA_ACIBL)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Acid345_2102

Organism

Acidobacteria bacterium (strain Ellin345) [Complete proteome] [HAMAP]

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019873
Sites
	Active site	293	1	Potential
	Active site	367	1	Potential
	Metal binding	281	1	Manganese 2 By similarity
	Metal binding	286	1	Manganese 1 By similarity
	Metal binding	286	1	Manganese 2 By similarity
	Metal binding	304	1	Manganese 2 By similarity
	Metal binding	363	1	Manganese 1 By similarity
	Metal binding	365	1	Manganese 1 By similarity
	Metal binding	365	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IPU7-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 13B78F4597A895D4	FASTA	512	54,376
	10 20 30 40 50 60 MKIHLSTLDP AQLETDALIV LAIDGGDKDN NKPQLQAKSD AFAKAAADLI ASKEITGKLL 70 80 90 100 110 120 EIATLHKPEG VKAKRLIVVG VGKAKSFTSY ELRKAAGAAV RALKKSVKSA AIVAPENWGG 130 140 150 160 170 180 AADPSTTSTL MFERGGLPEA VKAIAEGAVV ANPDYNYYHS DRKTYELDEL TILVPANGHA 190 200 210 220 230 240 NDLEAAMKEG HVIGESQNFT RDLVNEPGNR MTPTILGQRA KKMAEEVGIQ CDVYSTDFLH 250 260 270 280 290 300 EKKMGAFWSV SQGSEEPPAL IVMKYEPAGA PQSPVLGLVG KGITFDTGGI SIKPADGMEK 310 320 330 340 350 360 MKYDMAGGAA MIGAMRAIAL LKPNVRVIGV VCAAENMPSG KAQKPGDVQI AMSGKSIEII 370 380 390 400 410 420 NTDAEGRLVL ADGLHYAKQL GATHLIDAAT LTGACMVALG GINAGVFAND EDYFNRFAEA 430 440 450 460 470 480 LKKSGEKMWR LPIDDDYKEL IKSPIADIKN TGGRYGGAIT AAMFLKEFVG ETPWIHLDIA 490 500 510 GVAWQEEAVP FLAKGPSGIA VRSIIELVQS FG

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000360 Genomic DNA. Translation: ABF41103.1.
RefSeq	YP_591177.1.
3D structure databases
SMR	Q1IPU7. Positions 15-511.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IPU7.
Protein family/group databases
MEROPS	M17.010.
Genome annotation databases
GeneID	4069701.
GenomeReviews	Gene locus Acid345_2102 in contig CP000360_GR.
KEGG	aba:Acid345_2102.
NMPDR	fig|204669.6.peg.2087.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HBG742580.
OMA	EGHAIMA.
PhylomeDB	Q1IPU7.
Enzyme and pathway databases
BioCyc	ABAC204669:ACID345_2102-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ACIBL

Accession

Primary (citable) accession number: Q1IPU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)