Reviewed, UniProtKB/Swiss-Prot Q1IMB2 (GLPK_ACIBL)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Acid345_2987

Organism

Acidobacteria bacterium (strain Ellin345) [Complete proteome] [HAMAP]

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Glycerol kinase HAMAP MF_00186		PRO_1000020691
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IMB2-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 9D071F497142237E	FASTA	498	54,524
	10 20 30 40 50 60 MRLPYILALD QGTTSSRAIV FDQDGVVRSV AQREFTQIFP QAGWVEHDPE EIWASQISVA 70 80 90 100 110 120 VEALSRAELR SRHVAAIAIT NQRETAVVWD RETGRPVYNA IVWQDRRTAG FCDQLKAAGH 130 140 150 160 170 180 EEFVQQRTGL LIDSYFSGSK VRWILDNVPQ ARSLAERGRL AFGTVDSWLI WKLTSGKLHI 190 200 210 220 230 240 TDVSNASRTM LFNIHTCQWD EELLRLLEIP ASMLPEVRSS SEVYGTVQTS LGLESIPIAG 250 260 270 280 290 300 IAGDQQASLF GQRCTSPGMA KNTYGTGCFM LQNTGERAVP TSNRLVTTVA WKIGDVVEYA 310 320 330 340 350 360 LEGSVFIGGA VVQWLRDGLR LVRSSGEFQE LANSVKDNGG VYVVPAFVGL GAPHWDQYAR 370 380 390 400 410 420 GGIFGITRGT TDGHVARAAL ESIAYQVADL LDAMQRDTGV PLPELRVDGG AAANETLLQF 430 440 450 460 470 480 QADIMGVPVV RPSVTETTAL GAAFLAGIAV GLWSDPQVIA KMPVEQKRFE PRMGSAEALA 490 LRHQWEKALS RVKGWEEA

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000360 Genomic DNA. Translation: ABF41988.1.
RefSeq	YP_592062.1.
3D structure databases
SMR	Q1IMB2. Positions 3-498.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IMB2.
Genome annotation databases
GeneID	4068888.
GenomeReviews	Gene locus Acid345_2987 in contig CP000360_GR.
KEGG	aba:Acid345_2987.
NMPDR	fig|204669.6.peg.2985.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	TELKVDG.
PhylomeDB	Q1IMB2.
Enzyme and pathway databases
BioCyc	ABAC204669:ACID345_2987-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_ACIBL

Accession

Primary (citable) accession number: Q1IMB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)