Q1ILZ0 (ILVD_KORVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Acid345_3109

Organism

Koribacter versatilis (strain Ellin345)

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000321587
Sites
	Metal binding	128	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	200	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1ILZ0 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 1F61CFFDEA77B664	FASTA	573	60,752
	10 20 30 40 50 60 MTEKSPKPHK RSDAITEGPN RAPARAMLRA AGFTPEDLRK PIIGIANTWI EIGPCNLHLR 70 80 90 100 110 120 ELAEHIKQGV REAGGTPMEF NTVSISDGIT MGSEGMKASL VSREVIADSI ELVARGNLFD 130 140 150 160 170 180 GLIALSGCDK TIPGTIMALE RLDIPGLMLY GGSIAPGKFH AQKVTIQDVF EAVGTHARGK 190 200 210 220 230 240 MSDADLEELE HNACPGAGAC GGQFTANTMS MCGEFLGISP MGANSVPAMT VEKQQVARRC 250 260 270 280 290 300 GHLVMELVRR DIRPSQIITR KAIENAIASV AASGGSTNAV LHLLAIAHEM DVELNIEDFD 310 320 330 340 350 360 KISSRTPLLC ELKPAGRFTA TDLHDAGGIP LVAQRLLEAN LLHADALTVT GKTIAEEAKQ 370 380 390 400 410 420 AKETPGQEVV RPLTDPIKAT GGLMILKGNL ASEGCVVKLV GHKKLFFEGP ARVFESEEEA 430 440 450 460 470 480 FAGVEDRTIQ AGEVVVVRYE GPKGGPGMRE MLGVTAAIAG TELAETVALI TDGRFSGATR 490 500 510 520 530 540 GLSVGHVAPE AANGGAIAVV RNGDIITLDV ERRELRVHLT DAELEARLRN WRAPEPRYKR 550 560 570 GVFAKYASTV SSASFGAVTG STIENKTLAG STK

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ellin345.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000360 Genomic DNA. Translation: ABF42110.1.
RefSeq	YP_592184.1. NC_008009.1.
3D structure databases
ProteinModelPortal	Q1ILZ0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1ILZ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4070223.
GenomeReviews	Gene locus Acid345_3109 in contig CP000360_GR.
KEGG	aba:Acid345_3109.
NMPDR	fig|204669.6.peg.3112.
PATRIC	31983350. VBICanKor57425_3309.
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	Q1ILZ0.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	ABAC204669:ACID345_3109-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_KORVE

Accession

Primary (citable) accession number: Q1ILZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 13, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program