Reviewed, UniProtKB/Swiss-Prot Q1IKI5 (AROA_ACIBL)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	Acid345_3614

Organism

Acidobacteria bacterium (strain Ellin345) [Complete proteome] [HAMAP]

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm Probable HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000325327

Sequences

	Sequence		Length	Mass (Da)
	Q1IKI5-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 94C0C600B62C9983	FASTA	427	45,249
	10 20 30 40 50 60 MKSVRVQPAS NILGSVSFPG DKSISHRYGM LAALAEGTSR FKNFSTGADC ASTLSCMEQL 70 80 90 100 110 120 GAKVTHKDDG VIEVEGVAGQ LRKSATQLDC GNSGSSMRML SGILAAQPFD SELMGDASLS 130 140 150 160 170 180 RRPMRRIVDP LKQMGGDIET TDGHAPLRVR GSKLTAIDYI TPVPSAQVKS CVLFAGAFAA 190 200 210 220 230 240 GITSVDEAIR TRDHGEIALK AFGAEVERRQ NRVSVRGGAK FRAIEAVVPG DISSAAFFLC 250 260 270 280 290 300 AAALFPTSNL VFDGILLNPS RAAILDVLAS MGAKPKFLQV QEQHGELVGT ITLAPAGLSG 310 320 330 340 350 360 LKISGGLTAS LIDELPVLAA IGAYTRYGIE IRDAKELRVK ESDRIAVVCA NLRAMGAEVE 370 380 390 400 410 420 EFDDGLRVKG SQKLHGAEIE SHEDHRIAMA FAVAALRAEG ETVINGADCV AISYPEFFDT LNKVVER

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000360 Genomic DNA. Translation: ABF42615.1. Different initiation.
RefSeq	YP_592689.1.
3D structure databases
SMR	Q1IKI5. Positions 10-426.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IKI5.
Genome annotation databases
GeneID	4070134.
GenomeReviews	Gene locus Acid345_3614 in contig CP000360_GR.
KEGG	aba:Acid345_3614.
NMPDR	fig|204669.6.peg.3619.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HBG646626.
Enzyme and pathway databases
BioCyc	ABAC204669:ACID345_3614-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR016228. EPSP_synthase. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. False negative. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_ACIBL

Accession

Primary (citable) accession number: Q1IKI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	February 9, 2010
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)