Reviewed, UniProtKB/Swiss-Prot Q1IKG7 (MRAY_ACIBL)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	Acid345_3632

Organism

Acidobacteria bacterium (strain Ellin345) [Complete proteome] [HAMAP]

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000002927
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	76 – 96	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	135 – 155	21	Potential
	Transmembrane	185 – 205	21	Potential
	Transmembrane	218 – 238	21	Potential
	Transmembrane	255 – 275	21	Potential
	Transmembrane	283 – 303	21	Potential
	Transmembrane	307 – 327	21	Potential
	Transmembrane	356 – 376	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1IKG7-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: B48DC907E53519D1	FASTA	379	41,792
	10 20 30 40 50 60 MLYWLLYEKA FLLHNFTPFR IFRYLTFRTA FASLTALFMG LIIGPAVVRR LREFQIGQYI 70 80 90 100 110 120 REEGPKSHQK KSGTPTMGGV LITIAIIVPT LLWADLSNKF VWIAMLATIA FGAIGFTDDY 130 140 150 160 170 180 LKVANRRNLG LTARAKMGLQ ILVAILVAIS LVLVQRHGHY NTHLIVPFIK SFHPDLEISK 190 200 210 220 230 240 LATYPHIWII AYIPFLAFVA IVLVGSSNAV NLTDGLDGLA IGCTVIAAGA LTVLTYVSGH 250 260 270 280 290 300 AQFADYLGLS RMPEVGELSI FCGAMVGSAI GFLWYNAHPA EVFMGDVGSL ALGGAIGTVA 310 320 330 340 350 360 VIIKQELLLP FIGGVFVLEA LSVILQVGSY KLRKKRIFKM APLHHHFELL GWSESKIIVR 370 FWIASLVFAL FALTTLKLR

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000360 Genomic DNA. Translation: ABF42633.1.
RefSeq	YP_592707.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IKG7.
Genome annotation databases
GeneID	4070152.
GenomeReviews	Gene locus Acid345_3632 in contig CP000360_GR.
KEGG	aba:Acid345_3632.
NMPDR	fig|204669.6.peg.3637.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1IKG7.
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. False negative. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_ACIBL

Accession

Primary (citable) accession number: Q1IKG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)