Q1IJF8 (SYC_KORVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Acid345_3992

Organism

Koribacter versatilis (strain Ellin345)

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Cysteine--tRNA ligase HAMAP MF_00041		PRO_1000006558
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	211	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Metal binding	240	1	Zinc By similarity
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IJF8 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: DB5490ADE703450C	FASTA	495	56,043
	10 20 30 40 50 60 MALELFNTLS GKIETFQPLE DNEVRMYACG PTVYDYGHIG NFRTFIVVDT LRRFLKQSGY 70 80 90 100 110 120 KLKHVMNVTD VDDKIIRNAA RDGKTVQEYT AKYRKAFLED CEALNIEHPE LLVNATDHID 130 140 150 160 170 180 EMARFIKRME DLDVAYKTED GSYYFRIAKF PEYGKLSKKD FAGMNDGARV DVDEYEKDNA 190 200 210 220 230 240 RDFALWKAPK PGEAKWDTVI GSGRPGWHIE CSIMSMKYLG ESFDIHLGGE DLVFPHHENE 250 260 270 280 290 300 IAQSESVTHK KFANFWVHSR FLLVEGQKMS KSLGNFFTVR DLILMGHKPS SIRFLLMSVP 310 320 330 340 350 360 YRKQLNFTFD GLKQAAISVD RLRNFKRRIQ TEPFAEGTND KIGLMANETI TKIKAALDND 370 380 390 400 410 420 LNTAEALAPI FDLVRDVNAA ADAGEVKRGD VASLLEALQK FDEIFAVLND DDSGKVKFAV 430 440 450 460 470 480 DWANQEGKAD KISAETAEMA KAAGLSDEKV EALVAEHSAA RKAKDFKRSD AIRAELMESG 490 IILENTKDGV RWKRK

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ellin345.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000360 Genomic DNA. Translation: ABF42992.1.
RefSeq	YP_593066.1. NC_008009.1.
3D structure databases
ProteinModelPortal	Q1IJF8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IJF8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4071128.
GenomeReviews	Gene locus Acid345_3992 in contig CP000360_GR.
KEGG	aba:Acid345_3992.
NMPDR	fig|204669.6.peg.3975.
PATRIC	31985272. VBICanKor57425_4261.
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	IGNLRTY.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	ABAC204669:ACID345_3992-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_KORVE

Accession

Primary (citable) accession number: Q1IJF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program