Reviewed, UniProtKB/Swiss-Prot Q1IHQ3 (SYN_ACIBL)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Acid345_4597

Organism

Acidobacteria bacterium (strain Ellin345) [Complete proteome] [HAMAP]

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000072527

Sequences

	Sequence		Length	Mass (Da)
	Q1IHQ3-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 0B8D70FBB25C76D3	FASTA	442	50,283
	10 20 30 40 50 60 MEQAPVSTIH EIGKHDGQSV TIKGWLYNLR ESGKLLFPIF RDGTGLLQGV LFKKNVSPEL 70 80 90 100 110 120 FDLVKNLTQE SSVIVTGNVR AEQRAPGGYE MDVTALEVVQ RVPENEPYPI TPKEHGIEFL 130 140 150 160 170 180 MEHRHLWVRS QRQAAILRIR GEIVRSAHEF FDKQGFTLTE PPILTPAACE GTSTLFPVDY 190 200 210 220 230 240 FGDPAFLTQS GQLYIEATAM ALGKVYSFGP TFRAEKSKTR RHLTEFWMVE PEVAFAELDD 250 260 270 280 290 300 IMKLAEDFIT HIVKSVLERR RDDLKTIERD LSKLENIDTP FPRISYDEAV QMLQEGHAKG 310 320 330 340 350 360 ELESRFEWGG DLGSPDETYI SSKFDRPVMI HRYPVEVKAF YMEPDPNNSK VALCVDVLAP 370 380 390 400 410 420 EGYGEVIGGS QRMGSYEKLL ARIHEHQLPE ESFKWYLDLR KFGGVPHSGF GMGIERAVAW 430 440 ICGLDHVRET IPFARTLHRI YP

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000360 Genomic DNA. Translation: ABF43597.1.
RefSeq	YP_593671.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IHQ3.
Genome annotation databases
GeneID	4071542.
GenomeReviews	Gene locus Acid345_4597 in contig CP000360_GR.
KEGG	aba:Acid345_4597.
NMPDR	fig|204669.6.peg.4573.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1IHQ3.
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_ACIBL

Accession

Primary (citable) accession number: Q1IHQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 13, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)