Q1IHH4 (RPOB_KORVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	Acid345_4676

Organism

Koribacter versatilis (strain Ellin345)

Taxonomic identifier

204669 [NCBI]

Taxonomic lineage

Bacteria › Acidobacteria › Candidatus Koribacter

Protein attributes

Sequence length	1489 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1489	1489	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000300267

Sequences

	Sequence		Length	Mass (Da)
	Q1IHH4 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 6CD38A69992E125A	FASTA	1,489	167,265
	10 20 30 40 50 60 MAEKKIAFRN RQDFSKIPAT IQIPNLIEVQ KRSYDRFLQM DLLPSERDDA GLQAVFQSVF 70 80 90 100 110 120 PITDFRNVSQ LEFVDYAIGN WECKCGHLKG LHHLRTTCKN CGATVVTDPF HPGDVLCHKC 130 140 150 160 170 180 GTFNANTPDF CNKCGDPVGL QLKYDVAECE ERGMTYSAPL KVTMRLTIFD KDPETNNRTI 190 200 210 220 230 240 RDIKEQEVFF GDVPLMTQNG TFIINGTERV IVSQLHRSPG VFFETANNRT YFLGKIIPYR 250 260 270 280 290 300 GSWVEFEYDQ KNILYVRIDR KRKFLGTIFL RALGLRTDED ILRTFYTVDR IAVKDKKLYW 310 320 330 340 350 360 TLEPGIERPT NLVGLKLSHA IKAKNGEEVA HSGRKVTASV LKEIQKHKIS ELEIELGDLE 370 380 390 400 410 420 GAYVASDVID TNTGEVLLEA NQELTADKLS KMIDAGIGEV NVFFPERDDV GTVISATLRR 430 440 450 460 470 480 DSVKTPQEAL IEIYRKLRPG DPPTLDTATA LFHGMFFDAR KYDFSRVGRL KFNIKLFDRQ 490 500 510 520 530 540 DPTGLDKRTL DPDDFYHTIR YLLKLRRNLG AVDDIDHLGN RRVRAVGELL ENQFRIGLVR 550 560 570 580 590 600 MERAIKEKMS VYQEMSTAMP HDLVNAKPVM AAIREFFGSS QLSQFMDQTN PLSEITHKRR 610 620 630 640 650 660 LSALGPGGLS RERAGFEVRD VHPTHYGRIC PIETPEGPNI GLISSLSCYA RINDYGFIES 670 680 690 700 710 720 PYRRVKGGRV IDYVQVTHAG DSDYRVGDKM EKSEAQKANE ELRGRKKRGI ELEPYSFYLS 730 740 750 760 770 780 AWEEDKWTIA QANAELDEKG KITSELVNAR KAGNFVLISR DDIDYIDVSP KQLVSVAASL 790 800 810 820 830 840 VPFLEHDDAN RALMGANMQR QSVPLLRAEA PIVGTGMEGV TARDSGAVVL ARRSGIIDSV 850 860 870 880 890 900 DSERVIVRVE GEHHPMQLSR EVGSDIYQLT KFKRSNQNTC INQKPIVKQG DHVKKGQVIA 910 920 930 940 950 960 DGPCTDHGEL GLGRNVLVSF MPWRGYNFED AILVSEKLVK EDYYTSVHIE EFEIEARDTK 970 980 990 1000 1010 1020 LGPEEITRDI PNVSESALRD LDESGVIRIG APVKAGDILV GKVTPKGETQ LTPEEKLLRA 1030 1040 1050 1060 1070 1080 IFGEKAGDVR DASLTCPPGI EGVVVDVKIF SRKGQEKDER AKQIEGTQIA KLEKNLADEI 1090 1100 1110 1120 1130 1140 RILTDERLKR LEGLLGAKVV QADLHDERTN KRLLTKDAVL DRETIERIST RNLKRIKYAD 1150 1160 1170 1180 1190 1200 KDPRVNEQID EIEEMTSRQI DVLRKIVREK IEKLQKGDEL PPGVIKLVKV YIAMKRKLSV 1210 1220 1230 1240 1250 1260 GDKMAGRHGN KGVIARILPE EDMPYLEDGT PVEIVLNPLG VPSRMNVGQI LETHLGWAGH 1270 1280 1290 1300 1310 1320 ELGKKIAEFM VENSEAGQVR KHLKQLFKDT AFVDHVTELD DEMLLKVAKG MQDGVFFGSA 1330 1340 1350 1360 1370 1380 VFDGSTEAEI KSLLDQAGLP TSGKTFLYDG MTGDRFEQPV TVGYIYMLKL SHLVDDKIHA 1390 1400 1410 1420 1430 1440 RSIGPYSLIT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY ILQELLTAKS DDVYGRTKIY 1450 1460 1470 1480 EAIVKGEAAI EPGVPESFNV LIRELQSLCL DVELIKTKEK AAPAPVAAD

References

[1]

"Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils." Ward N.L., Challacombe J.F., Janssen P.H., Henrissat B., Coutinho P.M., Wu M., Xie G., Haft D.H., Sait M., Badger J., Barabote R.D., Bradley B., Brettin T.S., Brinkac L.M., Bruce D., Creasy T., Daugherty S.C., Davidsen T.M. , DeBoy R.T., Detter J.C., Dodson R.J., Durkin A.S., Ganapathy A., Gwinn-Giglio M., Han C.S., Khouri H., Kiss H., Kothari S.P., Madupu R., Nelson K.E., Nelson W.C., Paulsen I., Penn K., Ren Q., Rosovitz M.J., Selengut J.D., Shrivastava S., Sullivan S.A., Tapia R., Thompson L.S., Watkins K.L., Yang Q., Yu C., Zafar N., Zhou L., Kuske C.R. Appl. Environ. Microbiol. 75:2046-2056(2009) [PubMed: 19201974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ellin345.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000360 Genomic DNA. Translation: ABF43676.1.
RefSeq	YP_593750.1. NC_008009.1.
3D structure databases
ProteinModelPortal	Q1IHH4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1IHH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4070721.
GenomeReviews	Gene locus Acid345_4676 in contig CP000360_GR.
KEGG	aba:Acid345_4676.
NMPDR	fig|204669.6.peg.4654.
PATRIC	31986769. VBICanKor57425_4996.
Phylogenomic databases
eggNOG	COG0085.
HOGENOM	HBG285547.
OMA	IGRNKTY.
PhylomeDB	Q1IHH4.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	ABAC204669:ACID345_4676-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_KORVE

Accession

Primary (citable) accession number: Q1IHH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	June 13, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program