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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70S-adenosyl-L-methionineUniRule annotation1
Binding sitei95S-adenosyl-L-methionineUniRule annotation1
Binding sitei122S-adenosyl-L-methionineUniRule annotation1
Active sitei145By similarity1
Binding sitei145S-adenosyl-L-methionineUniRule annotation1
Binding sitei149SubstrateUniRule annotation1
Binding sitei181SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:PSEEN0308
OrganismiPseudomonas entomophila (strain L48)
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000658 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002882041 – 240tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST240

Interactioni

Protein-protein interaction databases

STRINGi384676.PSEEN0308.

Structurei

3D structure databases

ProteinModelPortaliQ1IGD2.
SMRiQ1IGD2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 221Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiPDPWHKS.
OrthoDBiPOG091H015P.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1IGD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESQETPIT EDGEARPHRR IKSFVMRAGR MTEGQQRGLE QGGPLFILPL
60 70 80 90 100
ADSPVDYDQV FGRSAPRTLE IGFGMGHSLL EMAAAAPELD FIGVEVHRPG
110 120 130 140 150
VGALLNGVLT QGLKNLRVYD CDAIEVLNRC VADNSLDRLM LFFPDPWHKA
160 170 180 190 200
RHHKRRIVQP EFAELVRRKL KVGGVFHMAT DWEPYAEHML EVMKVAPGYR
210 220 230 240
NQAADGAYVP RPEERPITKF ERRGERLGHG VWDLKFEKVD
Length:240
Mass (Da):27,194
Last modified:June 13, 2006 - v1
Checksum:iBAFB093740CF640A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573326 Genomic DNA. Translation: CAK13270.1.
RefSeqiWP_011531730.1. NC_008027.1.

Genome annotation databases

EnsemblBacteriaiCAK13270; CAK13270; PSEEN0308.
GeneIDi32803651.
KEGGipen:PSEEN0308.

Similar proteinsi

Entry informationi

Entry nameiTRMB_PSEE4
AccessioniPrimary (citable) accession number: Q1IGD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 13, 2006
Last modified: November 22, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families