Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q1IGD2 (TRMB_PSEE4) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:PSEEN0308
OrganismPseudomonas entomophila (strain L48) [Complete proteome] [HAMAP]
Taxonomic identifier384676 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288204

Regions

Region218 – 2214Substrate binding By similarity

Sites

Active site1451 By similarity
Binding site701S-adenosyl-L-methionine By similarity
Binding site951S-adenosyl-L-methionine By similarity
Binding site1221S-adenosyl-L-methionine By similarity
Binding site1451S-adenosyl-L-methionine By similarity
Binding site1491Substrate By similarity
Binding site1811Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1IGD2 [UniParc].

Last modified June 13, 2006. Version 1.
Checksum: BAFB093740CF640A

FASTA24027,194
        10         20         30         40         50         60 
MTESQETPIT EDGEARPHRR IKSFVMRAGR MTEGQQRGLE QGGPLFILPL ADSPVDYDQV 

        70         80         90        100        110        120 
FGRSAPRTLE IGFGMGHSLL EMAAAAPELD FIGVEVHRPG VGALLNGVLT QGLKNLRVYD 

       130        140        150        160        170        180 
CDAIEVLNRC VADNSLDRLM LFFPDPWHKA RHHKRRIVQP EFAELVRRKL KVGGVFHMAT 

       190        200        210        220        230        240 
DWEPYAEHML EVMKVAPGYR NQAADGAYVP RPEERPITKF ERRGERLGHG VWDLKFEKVD 

« Hide

References

[1]"Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila."
Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F.
Nat. Biotechnol. 24:673-679(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: L48.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CT573326 Genomic DNA. Translation: CAK13270.1.
RefSeqYP_606087.1. NC_008027.1.

3D structure databases

ProteinModelPortalQ1IGD2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING384676.PSEEN0308.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAK13270; CAK13270; PSEEN0308.
GeneID4087195.
KEGGpen:PSEEN0308.
PATRIC19858826. VBIPseEnt83862_0289.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycPENT384676:GJB8-280-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PSEE4
AccessionPrimary (citable) accession number: Q1IGD2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 13, 2006
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways