Reviewed, UniProtKB/Swiss-Prot Q1IG70 (BETA_PSEE4)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	PSEEN0372

Organism

Pseudomonas entomophila (strain L48) [Complete proteome] [HAMAP]

Taxonomic identifier

384676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Choline dehydrogenase		PRO_1000046568
Regions
	Nucleotide binding	6 – 35	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1IG70-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 6FF9E83214523B64	FASTA	565	62,392
	10 20 30 40 50 60 MSKEFDYIIV GAGSAGNTLA TRLTEDASVS VLLLEAGGPD YRFDFRTQMP AALAFPLQGR 70 80 90 100 110 120 RYNWAYETDP EPFMDGRRME CGRGKGLGGS SLINGMCYIR GNAMDFDGWA ELPGLEDWTY 130 140 150 160 170 180 LDCLPYFRKA ETRDIGPNDY HGGEGPVSVA TPKAGNNPLF HAMVEAGVQA GYPRTEDLNG 190 200 210 220 230 240 YQQEGFGPMD RSVTKKGRRS STARGYLDQA KKRPNLTIVT HALSDRVLFD GKRAVGVTYL 250 260 270 280 290 300 VGDSEERVEA RARKEVIVSS GAIASPQLLQ RSGVGPRALL ESLDIPVVHD LPGVGENLQD 310 320 330 340 350 360 HLELYLQYAC TQPVSLYPSL LWWNQPAIGA EWLFNGTGIG ASNQFEAGGF IRTRPEFKWP 370 380 390 400 410 420 NIQYHFLPVA INYNGSNGVK EHGFQAHMGS MRSPARGRIQ AKSKDPRQHP SILFNYMSTE 430 440 450 460 470 480 QDWQEFRDGI RLTREIMAQP ALDAFRGREI SPGAHVQTDE ELDKFIREHA ETAFHPSCSC 490 500 510 520 530 540 KMGTDDMAVV DGEGRVHGMQ GLRVVDASIM PLIITGNLNA TTIMIAEKIS DKIRGRKPLP 550 560 RSTAKYYVAG DAPVKGKALR EVKQA

References

[1]

"Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila." Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F. Nat. Biotechnol. 24:673-679(2006) [PubMed: 16699499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CT573326 Genomic DNA. Translation: CAK13332.1.
RefSeq	YP_606149.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4089376.
GenomeReviews	Gene locus PSEEN0372 in contig CT573326_GR.
KEGG	pen:PSEEN0372.
NMPDR	fig|384676.6.peg.325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1IG70.
Enzyme and pathway databases
BioCyc	PENT384676:PSEEN0372-MON.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_PSEE4

Accession

Primary (citable) accession number: Q1IG70

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)