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Protein

Argininosuccinate synthase

Gene

argG

Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei88CitrullineUniRule annotation1
Binding sitei93CitrullineUniRule annotation1
Binding sitei118ATP; via amide nitrogenUniRule annotation1
Binding sitei120AspartateUniRule annotation1
Binding sitei124AspartateUniRule annotation1
Binding sitei124CitrullineUniRule annotation1
Binding sitei125AspartateUniRule annotation1
Binding sitei128CitrullineUniRule annotation1
Binding sitei179CitrullineUniRule annotation1
Binding sitei188CitrullineUniRule annotation1
Binding sitei264CitrullineUniRule annotation1
Binding sitei276CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:PSEEN1210
OrganismiPseudomonas entomophila (strain L48)
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000658 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000004241 – 405Argininosuccinate synthaseAdd BLAST405

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi384676.PSEEN1210.

Structurei

3D structure databases

ProteinModelPortaliQ1IE03.
SMRiQ1IE03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiGVGRIDM.
OrthoDBiPOG091H01U0.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR11587. PTHR11587. 1 hit.
PfamiView protein in Pfam
PF00764. Arginosuc_synth. 1 hit.
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiView protein in PROSITE
PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1IE03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADVKKVVLA YSGGLDTSVI LKWLQDTYNC EVVTFTADLG QGEEVEPARA
60 70 80 90 100
KAQAMGVKEI YIDDLREEFV RDFVFPMFRA NTVYEGEYLL GTSIARPLIA
110 120 130 140 150
KRLIEIANET GADAISHGAT GKGNDQVRFE LGAYALKPGV KVIAPWREWD
160 170 180 190 200
LLSREKLMDY AEKHGIPIER HGKKKSPYSM DANLLHISYE GGVLEDTWTE
210 220 230 240 250
HEEDMWRWSV SPENAPDQAT YIELTYRNGD IVAIDGVEKS PATVLADLNR
260 270 280 290 300
IGGANGIGRL DIVENRYVGM KSRGCYETPG GTIMLKAHRA IESITLDREV
310 320 330 340 350
AHLKDELMPK YASLIYTGYW WSPERLMLQQ MIDASQVNVN GVVRLKLYKG
360 370 380 390 400
NVTVVGRKSD DSLFDANIAT FEEDGGAYNQ ADAAGFIKLN ALRMRIAANK

GRSLL
Length:405
Mass (Da):45,164
Last modified:June 13, 2006 - v1
Checksum:i69108EE1B1593A5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573326 Genomic DNA. Translation: CAK14105.1.
RefSeqiWP_003254941.1. NC_008027.1.

Genome annotation databases

EnsemblBacteriaiCAK14105; CAK14105; PSEEN1210.
GeneIDi32804492.
KEGGipen:PSEEN1210.

Similar proteinsi

Entry informationi

Entry nameiASSY_PSEE4
AccessioniPrimary (citable) accession number: Q1IE03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 13, 2006
Last modified: August 30, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families