Reviewed, UniProtKB/Swiss-Prot Q1ID19 (TPMT_PSEE4)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	PSEEN1581

Organism

Pseudomonas entomophila (strain L48) [Complete proteome] [HAMAP]

Taxonomic identifier

384676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000047211
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1ID19-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: A9CC782A010FB9AB	FASTA	216	24,050
	10 20 30 40 50 60 MEPAFWHKRW ADNQIGFHQL QVNPYLQAHW PALGLAPGAR VLVPLCGKSL DMLWLAAQGY 70 80 90 100 110 120 RVLGVELSRR AVEDFFTEHG LPAQVTQHGA FEAWRSDEVE IWCGDVFALR AEDLADCAGV 130 140 150 160 170 180 YDRAALIALP PEMRERYMAL LGAKLPTACR GVLVTLDYDQ ALIDGPPFSV PDAEVRAGFS 190 200 210 GWQVDEVEGL EILEDSPKFI KAGVSSLVER AYRLTR

References

[1]

"Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila." Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F. Nat. Biotechnol. 24:673-679(2006) [PubMed: 16699499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CT573326 Genomic DNA. Translation: CAK14442.1.
RefSeq	YP_607250.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1ID19.
Genome annotation databases
GeneID	4087190.
GenomeReviews	Gene locus PSEEN1581 in contig CT573326_GR.
KEGG	pen:PSEEN1581.
NMPDR	fig|384676.6.peg.1373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1ID19.
OMA	PPFAVSP.
Enzyme and pathway databases
BioCyc	PENT384676:PSEEN1581-MON.
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_PSEE4

Accession

Primary (citable) accession number: Q1ID19

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)