Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Siroheme synthase

Gene

cysG

Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.IEP:

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.IEP:
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.IEP:
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.IEP:
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.IEP:

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.IEP:
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.IEP:
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.IEP:
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.IEP:
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.IEP:
Proteins known to be involved in this subpathway in this organism are:
  1. Siroheme synthase (cysG)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225S-adenosyl-L-methionine; via carbonyl oxygenIEP:1
Active sitei248Proton acceptorIEP:1
Active sitei270Proton donorIEP:1
Binding sitei306S-adenosyl-L-methionine; via carbonyl oxygenIEP:1
Binding sitei383S-adenosyl-L-methionine; via amide nitrogenIEP:1
Binding sitei412S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenIEP:1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADIEP:2
Nucleotide bindingi43 – 44NADIEP:2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Methyltransferase, Oxidoreductase, Transferase
Biological processCobalamin biosynthesis, Porphyrin biosynthesis
LigandNAD, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseIEP:
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferaseIEP: (EC:2.1.1.107IEP:)
Short name:
Urogen III methylaseIEP:
Alternative name(s):
SUMTIEP:
Uroporphyrinogen III methylaseIEP:
Short name:
UROMIEP:
Precorrin-2 dehydrogenaseIEP: (EC:1.3.1.76IEP:)
Sirohydrochlorin ferrochelataseIEP: (EC:4.99.1.4IEP:)
Gene namesi
Name:cysGIEP:
Ordered Locus Names:PSEEN2217
OrganismiPseudomonas entomophila (strain L48)
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000658 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003305361 – 463Siroheme synthaseAdd BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei128PhosphoserineIEP:1

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi384676.PSEEN2217.

Structurei

3D structure databases

ProteinModelPortaliQ1IBC9.
SMRiQ1IBC9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseIEP:Add BLAST203
Regioni216 – 463Uroporphyrinogen-III C-methyltransferaseIEP:Add BLAST248
Regioni301 – 303S-adenosyl-L-methionine bindingIEP:3
Regioni331 – 332S-adenosyl-L-methionine bindingIEP:2

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.IEP:
In the C-terminal section; belongs to the precorrin methyltransferase family.IEP:

Phylogenomic databases

eggNOGiENOG4105CB8. Bacteria.
COG0007. LUCA.
COG1648. LUCA.
HOGENOMiHOG000290518.
KOiK02302.
OMAiQVSEYWP.
OrthoDBiPOG091H0526.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01646. Siroheme_synth. 1 hit.
InterProiView protein in InterPro
IPR000878. 4pyrrol_Mease.
IPR035996. 4pyrrol_Methylase_sf.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR036291. NAD(P)-bd_dom_sf.
IPR037115. Sirohaem_synt_dimer_dom_sf.
IPR012409. Sirohaem_synth.
IPR028281. Sirohaem_synthase_central.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
PfamiView protein in Pfam
PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF14824. Sirohm_synth_M. 1 hit.
PF00590. TP_methylase. 1 hit.
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiView protein in PROSITE
PS00840. SUMT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1IBC9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYLPLFHKL QGGRALVVGG GEIALRKARL LADAGAALRV VAPEVDGQLA
60 70 80 90 100
ALAREGGGEV LVRGYQSSDL DGCRLVIAAT DDPGLNAQVS AHAQARSLPV
110 120 130 140 150
NVVDAPALCT VIFPAIVDRS PLVVAVSSGG DAPVLARLIR AKLEAWIPSA
160 170 180 190 200
YGELAGLAAR FRDKVKALYP DVNQRRGFWE NVFQGPIAER QLAGQGAEAE
210 220 230 240 250
RLLQAMVDGA PVQQGGEVYL VGAGPGDPDL LTFRALRLMQ QADVVLYDRL
260 270 280 290 300
VAPAIIEMCR RDAERIYVGK RRADHAVPQD QINRLLVDLA RQGKRVLRLK
310 320 330 340 350
GGDPFIFGRG GEEIEELADE GIPFQVVPGI TAASGCSAYG GIPLTHRDYA
360 370 380 390 400
QSVRFVTGHL KDGTSNLPWN DLVAPAQTLV FYMGLVGLPT ICAELIRHGR
410 420 430 440 450
AASTPAALVQ QGTTRNQRVF TGTLADLPEL VARHEVHAPT LVIVGEVVKL
460
RDKLAWFEGA QNS
Length:463
Mass (Da):49,542
Last modified:June 13, 2006 - v1
Checksum:i16E6DFFA8575E0DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573326 Genomic DNA. Translation: CAK15036.1.
RefSeqiWP_011533438.1. NC_008027.1.

Genome annotation databases

EnsemblBacteriaiCAK15036; CAK15036; PSEEN2217.
GeneIDi32805415.
KEGGipen:PSEEN2217.

Similar proteinsi

Entry informationi

Entry nameiCYSG_PSEE4
AccessioniPrimary (citable) accession number: Q1IBC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 13, 2006
Last modified: November 22, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families