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Protein

LexA repressor

Gene

lexA

Organism
Pseudomonas entomophila (strain L48)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotation

Catalytic activityi

Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei88 – 892Cleavage; by autolysisUniRule annotation
Active sitei123 – 1231For autocatalytic cleavage activityUniRule annotation
Active sitei160 – 1601For autocatalytic cleavage activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi28 – 4821H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. serine-type endopeptidase activity Source: UniProtKB-HAMAP
  3. structural molecule activity Source: InterPro

GO - Biological processi

  1. DNA repair Source: UniProtKB-HAMAP
  2. DNA replication Source: UniProtKB-HAMAP
  3. negative regulation of transcription, DNA-templated Source: UniProtKB-HAMAP
  4. SOS response Source: UniProtKB-HAMAP
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation, RepressorUniRule annotationSAAS annotation

Keywords - Biological processi

DNA damage, DNA repair, DNA replicationUniRule annotationSAAS annotation, SOS responseUniRule annotationSAAS annotation, Transcription, Transcription regulationUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciPENT384676:GJB8-3522-MONOMER.

Protein family/group databases

MEROPSiS24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
LexA repressorUniRule annotationSAAS annotation (EC:3.4.21.88UniRule annotationSAAS annotation)
Gene namesi
Name:lexAUniRule annotationImported
Ordered Locus Names:PSEEN3721Imported
OrganismiPseudomonas entomophila (strain L48)Imported
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000658: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. viral capsid Source: InterPro
Complete GO annotation...

PTM / Processingi

Keywords - PTMi

Autocatalytic cleavageUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi384676.PSEEN3721.

Structurei

3D structure databases

ProteinModelPortaliQ1I7E1.
SMRiQ1I7E1. Positions 4-201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S24 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1974.
HOGENOMiHOG000232167.
KOiK01356.
OMAiIAETGMP.
OrthoDBiEOG6JHRHJ.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPiMF_00015. LexA.
InterProiIPR001742. Capsid_VP2_Orbivir.
IPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
ProDomiPD002938. Capsid_VP2_Orbivir. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1I7E1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKLTPRQAE ILAFIKRCLE DNGFPPTRAE IAQELGFKSP NAAEEHLKAL
60 70 80 90 100
ARKGAIEMTP GASRGIRIPG VEAKVEDNGL PIIGRVAAGA PILAEQHIEE
110 120 130 140 150
SCNINPAFFH PRADYLLRVH GMSMKDIGIF DGDLLAVHTT REARNGQVVV
160 170 180 190 200
ARIGDEVTVK RFKREGSKVW LIAENPEFAP IEVDLKEQEL VIEGLSVGVL

RR
Length:202
Mass (Da):22,218
Last modified:June 13, 2006 - v1
Checksum:iB3617BBF94707B85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573326 Genomic DNA. Translation: CAK16441.1.
RefSeqiYP_609228.1. NC_008027.1.

Genome annotation databases

EnsemblBacteriaiCAK16441; CAK16441; PSEEN3721.
GeneIDi4088987.
KEGGipen:PSEEN3721.
PATRICi19865516. VBIPseEnt83862_3576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573326 Genomic DNA. Translation: CAK16441.1.
RefSeqiYP_609228.1. NC_008027.1.

3D structure databases

ProteinModelPortaliQ1I7E1.
SMRiQ1I7E1. Positions 4-201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi384676.PSEEN3721.

Protein family/group databases

MEROPSiS24.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAK16441; CAK16441; PSEEN3721.
GeneIDi4088987.
KEGGipen:PSEEN3721.
PATRICi19865516. VBIPseEnt83862_3576.

Phylogenomic databases

eggNOGiCOG1974.
HOGENOMiHOG000232167.
KOiK01356.
OMAiIAETGMP.
OrthoDBiEOG6JHRHJ.

Enzyme and pathway databases

BioCyciPENT384676:GJB8-3522-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPiMF_00015. LexA.
InterProiIPR001742. Capsid_VP2_Orbivir.
IPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00726. LEXASERPTASE.
ProDomiPD002938. Capsid_VP2_Orbivir. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila."
    Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F.
    Nat. Biotechnol. 24:673-679(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L48Imported.

Entry informationi

Entry nameiQ1I7E1_PSEE4
AccessioniPrimary (citable) accession number: Q1I7E1
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2006
Last sequence update: June 13, 2006
Last modified: March 4, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.