Q1I6D1 (RIMO_PSEE4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal protein S12 methylthiotransferase RimO
Short name=S12 MTTase
Short name=S12 methylthiotransferase
EC=2.-.-.-

Alternative name(s):
Ribosome maturation factor RimO

Gene names

Name:	rimO
Ordered Locus Names:	PSEEN4113

Organism

Pseudomonas entomophila (strain L48) [Complete proteome] [HAMAP]

Taxonomic identifier

384676 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas entomophila

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 By similarity. HAMAP-Rule MF_01865
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01865.
Sequence similarities	Belongs to the methylthiotransferase family. RimO subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA modification Inferred from electronic annotation. Source: InterPro peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Ribosomal protein S12 methylthiotransferase RimO HAMAP-Rule MF_01865		PRO_0000374944
Regions
	Domain	8 – 118	111	MTTase N-terminal
	Domain	378 – 443	66	TRAM
Sites
	Metal binding	17	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	53	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	82	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	151	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	155	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	158	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1I6D1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 55EF840663A08828	FASTA	443	49,408
	10 20 30 40 50 60 MSTTPATPKV GFVSLGCPKA LVDSERILTQ LRMEGYEVVP TYEDADVVVV NTCGFIDSAK 70 80 90 100 110 120 AESLEVIGEA IKENGKVIVT GCMGVEEGSI RDVHPSVLSV TGPQQYEQVV NAVHEVVPPR 130 140 150 160 170 180 QDHNPLIDLV PPQGVKLTPR HYAYLKISEG CNHSCSFCII PSMRGKLVSR PVGEVLSEAE 190 200 210 220 230 240 RLVKAGVKEI LVISQDTSAY GVDVKYKTDF WNGRPVKTRM LELCEALSSL GAWVRLHYVY 250 260 270 280 290 300 PYPNVDDVIP LMAAGKILPY LDIPFQHASP KVLKSMKRPA FEDRTLARIK NWREQCPELV 310 320 330 340 350 360 IRSTFIVGFP GETEEDFQYL LDWLTEAQLD RVGCFQYSPV EGAPANDLGL DEVPDDVKQE 370 380 390 400 410 420 RWDRFMAHQQ AISAARLQQR IGKEIEVLID EVEEQGSVGR SFFDAPEIDG SVFIDGDHGF 430 440 KPGDKVRCRI VDADEYDMWA EPI

References

[1]

"Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila." Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F. Nat. Biotechnol. 24:673-679(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L48.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT573326 Genomic DNA. Translation: CAK16804.1.
RefSeq	YP_609588.1. NC_008027.1.
3D structure databases
ProteinModelPortal	Q1I6D1.
ModBase	Search...
Protein-protein interaction databases
STRING	384676.PSEEN4113.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAK16804; CAK16804; PSEEN4113.
GeneID	4089934.
KEGG	pen:PSEEN4113.
PATRIC	19866300. VBIPseEnt83862_3956.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0621.
HOGENOM	HOG000224766.
KO	K14441.
OMA	CPDITLR.
ProtClustDB	PRK14862.
Enzyme and pathway databases
BioCyc	PENT384676:GJB8-3897-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.80.30.20. 1 hit.
HAMAP	MF_01865. MTTase_RimO.
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR012340. NA-bd_OB-fold. IPR005840. Ribosomal_S12_MeSTrfase_RimO. IPR007197. rSAM. IPR023404. rSAM_horseshoe. IPR002792. TRAM_dom. [Graphical view]
PANTHER	PTHR11918. PTHR11918. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00089. TIGR00089. 1 hit. TIGR01125. TIGR01125. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIMO_PSEE4

Accession

Primary (citable) accession number: Q1I6D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	June 13, 2006
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program