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Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.UniRule annotation

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi53Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi151Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi155Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi158Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPENT384676:G1GI5-3863-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimOUniRule annotation (EC:2.8.4.4UniRule annotation)
Short name:
S12 MTTaseUniRule annotation
Short name:
S12 methylthiotransferaseUniRule annotation
Alternative name(s):
Ribosomal protein S12 (aspartate-C(3))-methylthiotransferaseUniRule annotation
Ribosome maturation factor RimOUniRule annotation
Gene namesi
Name:rimOUniRule annotation
Ordered Locus Names:PSEEN4113
OrganismiPseudomonas entomophila (strain L48)
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000658 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003749441 – 443Ribosomal protein S12 methylthiotransferase RimOAdd BLAST443

Proteomic databases

PRIDEiQ1I6D1

Interactioni

Protein-protein interaction databases

STRINGi384676.PSEEN4113

Structurei

3D structure databases

ProteinModelPortaliQ1I6D1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 118MTTase N-terminalUniRule annotationAdd BLAST111
Domaini378 – 443TRAMUniRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the methylthiotransferase family. RimO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CBM Bacteria
COG0621 LUCA
HOGENOMiHOG000224766
KOiK14441
OMAiCLSERYK
OrthoDBiPOG091H006S

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01865 MTTase_RimO, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR005840 Ribosomal_S12_MeSTrfase_RimO
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PANTHERiPTHR43837 PTHR43837, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00274 ribosomal_protein_S12_methylth, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
TIGR01125 TIGR01125, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

Q1I6D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTPATPKV GFVSLGCPKA LVDSERILTQ LRMEGYEVVP TYEDADVVVV
60 70 80 90 100
NTCGFIDSAK AESLEVIGEA IKENGKVIVT GCMGVEEGSI RDVHPSVLSV
110 120 130 140 150
TGPQQYEQVV NAVHEVVPPR QDHNPLIDLV PPQGVKLTPR HYAYLKISEG
160 170 180 190 200
CNHSCSFCII PSMRGKLVSR PVGEVLSEAE RLVKAGVKEI LVISQDTSAY
210 220 230 240 250
GVDVKYKTDF WNGRPVKTRM LELCEALSSL GAWVRLHYVY PYPNVDDVIP
260 270 280 290 300
LMAAGKILPY LDIPFQHASP KVLKSMKRPA FEDRTLARIK NWREQCPELV
310 320 330 340 350
IRSTFIVGFP GETEEDFQYL LDWLTEAQLD RVGCFQYSPV EGAPANDLGL
360 370 380 390 400
DEVPDDVKQE RWDRFMAHQQ AISAARLQQR IGKEIEVLID EVEEQGSVGR
410 420 430 440
SFFDAPEIDG SVFIDGDHGF KPGDKVRCRI VDADEYDMWA EPI
Length:443
Mass (Da):49,408
Last modified:June 13, 2006 - v1
Checksum:i55EF840663A08828
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573326 Genomic DNA Translation: CAK16804.1
RefSeqiWP_011535175.1, NC_008027.1

Genome annotation databases

EnsemblBacteriaiCAK16804; CAK16804; PSEEN4113
GeneIDi32807128
KEGGipen:PSEEN4113

Similar proteinsi

Entry informationi

Entry nameiRIMO_PSEE4
AccessioniPrimary (citable) accession number: Q1I6D1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 13, 2006
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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