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Q1I624

- GLND_PSEE4

UniProt

Q1I624 - GLND_PSEE4

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPENT384676:GJB8-4005-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:PSEEN4222
OrganismiPseudomonas entomophila (strain L48)
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000658: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022351Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi384676.PSEEN4222.

Structurei

3D structure databases

ProteinModelPortaliQ1I624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini481 – 590110HDUniRule annotationAdd
BLAST
Domaini706 – 78479ACT 1UniRule annotationAdd
BLAST
Domaini816 – 90085ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseAdd
BLAST
Regioni343 – 705363Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1I624-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRMAGEVLD KRFREGREIR
60 70 80 90 100
RLIEDRAWFV DNILQQAWNQ FDWGNPDGIA LVAVGGYGRG ELHPYSDIDL
110 120 130 140 150
LILLDAAEHE QYRDAIERFL TLLWDIGLEV GQSVRTVDEC AEQARADLTV
160 170 180 190 200
ITNLMESRTI AGPEPLRQRM LDATSTAHMW PSKEFFLAKR AELKARHHKY
210 220 230 240 250
NDTEYNLEPN VKGGPGGLRD IQTVLWVARR QYGTLNLHAL AGEDFLLESE
260 270 280 290 300
NELLASSQAF LWRVRYALHM LAGRAEDRLL FDHQRSIATL LGFNDENPKR
310 320 330 340 350
AIEQFMQQYY RVVMSISQLC DLIIQHFEEV ILADDDSGTT QPLNARFRLH
360 370 380 390 400
DGYIEAVHPN VFRRTPFAML EIFVLMAQHP EIKGVRADTV RLLREHRHLI
410 420 430 440 450
DDKFRNDIRN TSLFIELFKC EIGIHRNLRR MNRYGILGRY LPEFGLIVGQ
460 470 480 490 500
MQHDLFHIYT VDAHTLNLIK HLRKLQYTPV SEKFPLASKL MGRLPKPELI
510 520 530 540 550
YLAGLYHDIG KGRQGDHSEL GAVDAHAFCA RHQLPAWDSR LIVWLVLNHL
560 570 580 590 600
VMSTTAQRKD LSDPQVINDF ALHVGDETRL DYLYVLTVAD INATNPSLWN
610 620 630 640 650
SWRASLLRQL YTETKRALRR GLENPLDREE QIRQTQSAAL DILVREGTDP
660 670 680 690 700
DDVEQLWSQL GDDYFLKHNA ADVAWHSDAI LQQPADSGPL VLIKETTQRE
710 720 730 740 750
FEGGTQIFIY APDQHDFFAV TVAAMAQLNL NIHDARIITS SSQFTLDTYI
760 770 780 790 800
VLDNDGGSIG DNPQRVKQIR DGLTEALRTP EDYPAIIQRR VPRQLKHFDF
810 820 830 840 850
PPQVTILNDA QRPVTILEIT APDRPGLLAR IGRIFLEFDI SLQNAKIATL
860 870 880 890 900
GERVEDVFFI TDADNQPLSD PQLCSRLQEA IIQQLQAGQA SEASPSRMTF
Length:900
Mass (Da):102,904
Last modified:June 13, 2006 - v1
Checksum:i111FE5F5C52DC290
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT573326 Genomic DNA. Translation: CAK16911.1.
RefSeqiYP_609695.1. NC_008027.1.

Genome annotation databases

EnsemblBacteriaiCAK16911; CAK16911; PSEEN4222.
GeneIDi4089435.
KEGGipen:PSEEN4222.
PATRICi19866504. VBIPseEnt83862_4058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT573326 Genomic DNA. Translation: CAK16911.1 .
RefSeqi YP_609695.1. NC_008027.1.

3D structure databases

ProteinModelPortali Q1I624.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 384676.PSEEN4222.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAK16911 ; CAK16911 ; PSEEN4222 .
GeneIDi 4089435.
KEGGi pen:PSEEN4222.
PATRICi 19866504. VBIPseEnt83862_4058.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PENT384676:GJB8-4005-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila."
    Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F.
    Nat. Biotechnol. 24:673-679(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L48.

Entry informationi

Entry nameiGLND_PSEE4
AccessioniPrimary (citable) accession number: Q1I624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 13, 2006
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3