Q1I610 (PLSB_PSEE4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	PSEEN4236

Organism

Pseudomonas entomophila (strain L48) [Complete proteome] [HAMAP]

Taxonomic identifier

384676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	828 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 828	828	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049444
Regions
	Motif	309 – 314	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q1I610 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: FC1A95361CE8CB31	FASTA	828	94,000
	10 20 30 40 50 60 MTRSPLRRLI FGALRRVLYL WVRSETINQS SLTLKLDRSR PVFYALSSPS LTDLAVVDHE 70 80 90 100 110 120 CTKAGLPRPV LPVAVGPLQE PAGFFYLTPD PDWLGRQDKR GAPPTLERLV AAISQHAEED 130 140 150 160 170 180 TQIIPVSVFW GQTPASESSP WKLLFADSWA VTGRLRRLLS VLILGRKTRV QFSAPIHLRE 190 200 210 220 230 240 LVDHNKGHER TVRMAQRVMR VHFRNLKTAV IGPDISHRRN LVKGLVHDPL VRQAISDEAE 250 260 270 280 290 300 REKIPYAKAE AKALHYGNEI ASDYTYTAIR FLEVVLSWFW NKIYDGVKVN HIEQVQGIAP 310 320 330 340 350 360 GHEVIYVPCH RSHIDYLLLS YLLFRNGLTP PHIAAGINLN MPVIGGLLRR GGAFFMRRTF 370 380 390 400 410 420 KGNPLYTAVF NEYLHTLFTK GFPVEYFVEG GRSRTGRMLQ PRTGMLAITL RSFLRSSRTP 430 440 450 460 470 480 IVFVPVYIGY ERVLEGRTYL GELRGASKKK ESIFDIFKVI GALKQRFGQV YVNFGEPIRL 490 500 510 520 530 540 AGFLDEQQPG WREQELGPQF RPTWLNETTT RLGETVARHL NEAAAINPVN LVALALLSTS 550 560 570 580 590 600 RLALDERALT RVLDLYLALL RQVPYSPHTT LPEGDGQALI KHVLGMDLLA EQKDAMGRIL 610 620 630 640 650 660 YLDEANAVLM TYYRNNVLHI FALPGLLASF FLSSSRMSRD LLGQYVRALY PYLQAELFLR 670 680 690 700 710 720 WTPEQLDEVI DQWLAALVSQ GLLRQENDIY MRPAPSSRQF VLLTLLARTI TQTLQRFYMA 730 740 750 760 770 780 TSLLLNSGQH TLSAEELEEL CVMMAQRLSI LHGLNAPEFF DKTLFRHFIQ TLVREGVLQP 790 800 810 820 DGDGKLGYHD KLAELAEGVA KRVLSAELRL SIRQVALHRD EPQENPET

References

[1]

"Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila." Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F. Nat. Biotechnol. 24:673-679(2006) [PubMed: 16699499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L48.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT573326 Genomic DNA. Translation: CAK16925.1.
RefSeq	YP_609709.1. NC_008027.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1I610.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4086562.
GenomeReviews	Gene locus PSEEN4236 in contig CT573326_GR.
KEGG	pen:PSEEN4236.
NMPDR	fig|384676.6.peg.3651.
PATRIC	19866532. VBIPseEnt83862_4072.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Enzyme and pathway databases
BioCyc	PENT384676:PSEEN4236-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_PSEE4

Accession

Primary (citable) accession number: Q1I610

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program