Reviewed, UniProtKB/Swiss-Prot Q1I500 (HGD_PSEE4)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	PSEEN4612

Organism

Pseudomonas entomophila (strain L48) [Complete proteome] [HAMAP]

Taxonomic identifier

384676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O(2) = 4-maleylacetoacetate.
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Homogentisate 1,2-dioxygenase		PRO_1000019534
Sites
	Metal binding	331	1	Iron By similarity
	Metal binding	337	1	Iron By similarity
	Metal binding	367	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1I500-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: D8D621C6F6C28220	FASTA	433	48,300
	10 20 30 40 50 60 MTRDTSPQLQ YLSGFGNEFA SEALPGALPV GQNSPQKAPY GLYAELLSGT AFTMTRSELR 70 80 90 100 110 120 RTWLYRIRPS ALHPRFERLE RQPLTGALGA ITPNRLRWSP QPMPAEPTDF IEGWLPMVAN 130 140 150 160 170 180 SASEKPAGVS IYIYRANRSM ERVFFNADGE LLLVPEQGRL RIATELGVLE VEPLEIAVIP 190 200 210 220 230 240 RGMKFRVELL DDQARGYIAE NHGAPLRIPD LGPIGSNGLA NPRDFLTPVA HYEETSGPVQ 250 260 270 280 290 300 LVQKFLGEHW ACELQHSPLD VVAWHGSNVP YKYDLRRFNT IGTVSFDHPD PSIFTVLTSP 310 320 330 340 350 360 TSVHGMANMD FVIFPPRWMV AENTFRPPWF HRNLMNEFMG LIQGAYDAKA EGFLPGGASL 370 380 390 400 410 420 HGVMSAHGPD AETCDKAISV DLAPHKIDNT MAFMFETSQV LRPSRQALES PQLQADYDSC 430 WATLPSTFNP NRR

References

[1]

"Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila." Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F. Nat. Biotechnol. 24:673-679(2006) [PubMed: 16699499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CT573326 Genomic DNA. Translation: CAK17286.1.
RefSeq	YP_610069.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4089231.
GenomeReviews	Gene locus PSEEN4612 in contig CT573326_GR.
KEGG	pen:PSEEN4612.
NMPDR	fig|384676.6.peg.3983.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q1I500.
Enzyme and pathway databases
BioCyc	PENT384676:PSEEN4612-MON.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEE4

Accession

Primary (citable) accession number: Q1I500

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 13, 2006
Last modified:	November 4, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)