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Q1I4S5

- SYI_PSEE4

UniProt

Q1I4S5 - SYI_PSEE4

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Protein

Isoleucine--tRNA ligase

Gene
ileS, PSEEN4692
Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylate By similarity
Binding sitei611 – 6111ATP By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity
Metal bindingi929 – 9291Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPENT384676:GJB8-4461-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PSEEN4692
OrganismiPseudomonas entomophila (strain L48)
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000658: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligaseUniRule annotationPRO_1000022105Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi384676.PSEEN4692.

Structurei

3D structure databases

ProteinModelPortaliQ1I4S5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi608 – 6125"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1I4S5-1 [UniParc]FASTAAdd to Basket

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MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYGK LREIGKDRPK    50
FVLHDGPPYA NGKIHIGHAL NKILKDMIVR SKTLSGFDAP YVPGWDCHGL 100
PIEHKVEVTH GKHLTADRTR ELCREYAAEQ IEGQKTEFIR LGVLGDWDNP 150
YKTMNFANEA GEIRALAEMV KQGFVFKGLK PVNWCFDCGS ALAEAEVEYA 200
DKKSQTIDVA FPVADADKLA AAFGLSALAK PAAIVIWTTT PWTIPANQAL 250
NIHPDFKYAL VDTGERLLVL AEELVESCLK RYNLEGSVIA TAQGSALELI 300
NFRHPFYDRL SPIYLAEYVE LGAGTGVVHS SPAYGEDDFV TCKRYGMVNE 350
DILTPVQSNG VYVESLPFFG GQFIWKANPA IVDKLSEVGA LMHTETISHS 400
YMHCWRHKTP LIYRATAQWF VGMDKQPSTG EPLRERALKA IEETKFVPAW 450
GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LDKQTGELHP RTVELMEEVA 500
KRVEQEGIEA WFKLDAQELL GDEAGQYDKI TDTLDVWFDS GTTHWHVLRG 550
SHDIGHATGP RADLYLEGSD QHRGWFHSSL LTGCAIDGHA PYRELLTHGF 600
TVDENGRKMS KSLGNTIEPQ KVNDTLGADI LRLWVSATDY SGEMAVSEQI 650
LQRSADAYRR IRNTARFLLS NLSGFDPARD LLPAEDMLAL DRWAVDRTLL 700
LQRELEEHYS EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSVA 750
RRSCQTALYH ISEALVRWIA PILAFTADEI WQYLPGERNE SVMLNTWYEG 800
LSELPADAEL DRAYWDRVMA VKAAVNKELE NQRTAKVIGG NLQAEVTLFA 850
EEGLTADLNK LGDELRFVLI TSAASVVPFV QAPADAVTTE VEGLKLKVVK 900
SGHAKCGRCW HFRADVGSHP EHPEICGRCV DNLNGSGEVR HYA 943
Length:943
Mass (Da):105,922
Last modified:June 13, 2006 - v1
Checksum:iDF8EB485691B63A3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT573326 Genomic DNA. Translation: CAK17361.1.
RefSeqiWP_011535726.1. NC_008027.1.
YP_610144.1. NC_008027.1.

Genome annotation databases

EnsemblBacteriaiCAK17361; CAK17361; PSEEN4692.
GeneIDi4089076.
KEGGipen:PSEEN4692.
PATRICi19867406. VBIPseEnt83862_4504.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT573326 Genomic DNA. Translation: CAK17361.1 .
RefSeqi WP_011535726.1. NC_008027.1.
YP_610144.1. NC_008027.1.

3D structure databases

ProteinModelPortali Q1I4S5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 384676.PSEEN4692.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAK17361 ; CAK17361 ; PSEEN4692 .
GeneIDi 4089076.
KEGGi pen:PSEEN4692.
PATRICi 19867406. VBIPseEnt83862_4504.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PENT384676:GJB8-4461-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila."
    Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F.
    Nat. Biotechnol. 24:673-679(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L48.

Entry informationi

Entry nameiSYI_PSEE4
AccessioniPrimary (citable) accession number: Q1I4S5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 13, 2006
Last modified: September 3, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi