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Q1I4S5

- SYI_PSEE4

UniProt

Q1I4S5 - SYI_PSEE4

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (13 Jun 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
    Binding sitei611 – 6111ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPENT384676:GJB8-4461-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PSEEN4692
    OrganismiPseudomonas entomophila (strain L48)
    Taxonomic identifieri384676 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000658: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000022105Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi384676.PSEEN4692.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1I4S5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi608 – 6125"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKKRIELM.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1I4S5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDYKATLNL PDTAFPMKAG LPQREPQILQ RWDSIGLYGK LREIGKDRPK    50
    FVLHDGPPYA NGKIHIGHAL NKILKDMIVR SKTLSGFDAP YVPGWDCHGL 100
    PIEHKVEVTH GKHLTADRTR ELCREYAAEQ IEGQKTEFIR LGVLGDWDNP 150
    YKTMNFANEA GEIRALAEMV KQGFVFKGLK PVNWCFDCGS ALAEAEVEYA 200
    DKKSQTIDVA FPVADADKLA AAFGLSALAK PAAIVIWTTT PWTIPANQAL 250
    NIHPDFKYAL VDTGERLLVL AEELVESCLK RYNLEGSVIA TAQGSALELI 300
    NFRHPFYDRL SPIYLAEYVE LGAGTGVVHS SPAYGEDDFV TCKRYGMVNE 350
    DILTPVQSNG VYVESLPFFG GQFIWKANPA IVDKLSEVGA LMHTETISHS 400
    YMHCWRHKTP LIYRATAQWF VGMDKQPSTG EPLRERALKA IEETKFVPAW 450
    GQARLHSMIA NRPDWCISRQ RNWGVPIPFF LDKQTGELHP RTVELMEEVA 500
    KRVEQEGIEA WFKLDAQELL GDEAGQYDKI TDTLDVWFDS GTTHWHVLRG 550
    SHDIGHATGP RADLYLEGSD QHRGWFHSSL LTGCAIDGHA PYRELLTHGF 600
    TVDENGRKMS KSLGNTIEPQ KVNDTLGADI LRLWVSATDY SGEMAVSEQI 650
    LQRSADAYRR IRNTARFLLS NLSGFDPARD LLPAEDMLAL DRWAVDRTLL 700
    LQRELEEHYS EYRFWNVYSK VHNFCVQELG GFYLDIIKDR QYTTGANSVA 750
    RRSCQTALYH ISEALVRWIA PILAFTADEI WQYLPGERNE SVMLNTWYEG 800
    LSELPADAEL DRAYWDRVMA VKAAVNKELE NQRTAKVIGG NLQAEVTLFA 850
    EEGLTADLNK LGDELRFVLI TSAASVVPFV QAPADAVTTE VEGLKLKVVK 900
    SGHAKCGRCW HFRADVGSHP EHPEICGRCV DNLNGSGEVR HYA 943
    Length:943
    Mass (Da):105,922
    Last modified:June 13, 2006 - v1
    Checksum:iDF8EB485691B63A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT573326 Genomic DNA. Translation: CAK17361.1.
    RefSeqiWP_011535726.1. NC_008027.1.
    YP_610144.1. NC_008027.1.

    Genome annotation databases

    EnsemblBacteriaiCAK17361; CAK17361; PSEEN4692.
    GeneIDi4089076.
    KEGGipen:PSEEN4692.
    PATRICi19867406. VBIPseEnt83862_4504.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT573326 Genomic DNA. Translation: CAK17361.1 .
    RefSeqi WP_011535726.1. NC_008027.1.
    YP_610144.1. NC_008027.1.

    3D structure databases

    ProteinModelPortali Q1I4S5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 384676.PSEEN4692.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAK17361 ; CAK17361 ; PSEEN4692 .
    GeneIDi 4089076.
    KEGGi pen:PSEEN4692.
    PATRICi 19867406. VBIPseEnt83862_4504.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KKRIELM.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PENT384676:GJB8-4461-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila."
      Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F.
      Nat. Biotechnol. 24:673-679(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: L48.

    Entry informationi

    Entry nameiSYI_PSEE4
    AccessioniPrimary (citable) accession number: Q1I4S5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3