Q1I4P2 (PANB2_PSEE4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase 2
EC=2.1.2.11

Alternative name(s):
Ketopantoate hydroxymethyltransferase 2
Short name=KPHMT 2

Gene names

Name:	panB2
Ordered Locus Names:	PSEEN4735

Organism

Pseudomonas entomophila (strain L48) [Complete proteome] [HAMAP]

Taxonomic identifier

384676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the PanB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	3-methyl-2-oxobutanoate hydroxymethyltransferase 2 HAMAP MF_00156		PRO_0000297336
Regions
	Region	45 – 46	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	181	1	Proton acceptor By similarity
	Metal binding	45	1	Magnesium By similarity
	Metal binding	84	1	Magnesium By similarity
	Metal binding	114	1	Magnesium By similarity
	Binding site	84	1	Alpha-ketoisovalerate By similarity
	Binding site	112	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1I4P2 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: FD24D0EC04089EC4	FASTA	266	27,752
	10 20 30 40 50 60 MPEVTLTTLN GLKAKGEKIT MLTCYDATFA KAASEAGVEV LLVGDSLGMV LQGHDSTLPV 70 80 90 100 110 120 SNDDMAYHTA SVKRGNNGAL ILTDLPFMAH ATPELAFTNA AQLMRAGAHM VKIEGAAWLA 130 140 150 160 170 180 ETIRLLAERG VPVCAHMGLT PQTVNVLGGY KVQGRQEAQA RQMRADAIAL EQAGAAMLLL 190 200 210 220 230 240 ECVPSELAAE ITQAVGIPVI GIGAGSATDG QVLVLHDMLG LSLSGRVPKF VKNFMAGQPD 250 260 IQSALAAYVE AVKTVSFPAS EHGFSA

References

[1]

"Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila." Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F. Nat. Biotechnol. 24:673-679(2006) [PubMed: 16699499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L48.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT573326 Genomic DNA. Translation: CAK17394.1.
RefSeq	YP_610177.1. NC_008027.1.
3D structure databases
ProteinModelPortal	Q1I4P2.
SMR	Q1I4P2. Positions 4-265.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1I4P2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4085991.
GenomeReviews	Gene locus PSEEN4735 in contig CT573326_GR.
KEGG	pen:PSEEN4735.
NMPDR	fig|384676.6.peg.4077.
PATRIC	19867484. VBIPseEnt83862_4539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0413.
HOGENOM	HBG299908.
OMA	YDATFAH.
PhylomeDB	Q1I4P2.
ProtClustDB	PRK00311.
Enzyme and pathway databases
BioCyc	PENT384676:PSEEN4735-MONOMER.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K00606.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR00222. PanB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB2_PSEE4

Accession

Primary (citable) accession number: Q1I4P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	June 13, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program