Reviewed, UniProtKB/Swiss-Prot Q1I4K4 (RLMG_PSEE4)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	PSEEN4774

Organism

Pseudomonas entomophila (strain L48) [Complete proteome] [HAMAP]

Taxonomic identifier

384676 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366475

Sequences

	Sequence		Length	Mass (Da)
	Q1I4K4-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: D9A9AD4C34A2DF71	FASTA	374	40,524
	10 20 30 40 50 60 MPLFNSPFAD LDLIRQPEQA NDPLLAFDAA DQYLLEHLAA QAPAANCKVL VLNDSFGALA 70 80 90 100 110 120 ASLAGRLEVI SSGDSHLARM ALEKNLARNG KGFDSVPFMP ANETWQGPFD RVLVRVPKTL 130 140 150 160 170 180 ALLEEQLIRL QGQLAPGAQV IAGAMIKHLP RAAGDLMEKY IGPVQASLAQ KKARLLTATV 190 200 210 220 230 240 AERPLASSPY PSRYRLDSPA LELVNHANVF CREGLDIGTR AFLPHLPRNL GQARVADLGC 250 260 270 280 290 300 GNGVLAIASA LANPDAHYTL VDESYMAVQS ARDNWQAALG ERAVEIHAAD GLAGQEKQSL 310 320 330 340 350 360 EVVLCNPPFH QQQVVGDFLA WRMFQQAREA LVVGGALYIV GNRHLGYHSK LARLFRGVEQ 370 VAATPKFVVL KARK

References

[1]

"Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila." Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F. Nat. Biotechnol. 24:673-679(2006) [PubMed: 16699499] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT573326 Genomic DNA. Translation: CAK17432.1.
RefSeq	YP_610215.1.
3D structure databases
SMR	Q1I4K4. Positions 26-373.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1I4K4.
Genome annotation databases
GeneID	4086076.
GenomeReviews	Gene locus PSEEN4774 in contig CT573326_GR.
KEGG	pen:PSEEN4774.
NMPDR	fig|384676.6.peg.4116.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	CGNGVLG.
PhylomeDB	Q1I4K4.
Enzyme and pathway databases
BioCyc	PENT384676:PSEEN4774-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSEE4

Accession

Primary (citable) accession number: Q1I4K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 13, 2006
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)