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Protein

Lipoyl synthase

Gene

lipA

Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

[4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi84Iron-sulfur 1 (4Fe-4S)1
Metal bindingi89Iron-sulfur 1 (4Fe-4S)1
Metal bindingi95Iron-sulfur 1 (4Fe-4S)1
Metal bindingi110Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi114Iron-sulfur 2 (4Fe-4S-S-AdoMet)1
Metal bindingi117Iron-sulfur 2 (4Fe-4S-S-AdoMet)1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:PSEEN4819
OrganismiPseudomonas entomophila (strain L48)
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000658 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000122561 – 338Lipoyl synthaseAdd BLAST338

Interactioni

Protein-protein interaction databases

STRINGi384676.PSEEN4819.

Structurei

3D structure databases

ProteinModelPortaliQ1I4G1.
SMRiQ1I4G1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Phylogenomic databases

eggNOGiENOG4105C0G. Bacteria.
COG0320. LUCA.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPYCDIDF.
OrthoDBiPOG091H069D.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1I4G1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTVQEAVPN LIPTQDVTPR PAPKKVEAGV KLRGAEKVAR IPVKIIPTEE
60 70 80 90 100
LPKKPDWIRV RIPVSPEVDR IKQLLRKHKL HSVCEEASCP NLGECFSGGT
110 120 130 140 150
ATFMIMGDIC TRRCPFCDVG HGRPKPLDLD EPKNLAIAIA DLRLKYVVIT
160 170 180 190 200
SVDRDDLRDG GAQHFADCIR EIRALSPGVQ LETLVPDYRG RMDVALEITA
210 220 230 240 250
QTPPDVFNHN LETVPRLYKA ARPGSDYDWS LDLLQKFKQM VPHVPTKSGL
260 270 280 290 300
MLGLGETDEE VIEVMHRMRE HDIDMLTLGQ YLQPSRNHLP VQRFVHPDTF
310 320 330
AWFAEEGYKM GFKNVASGPL VRSSYHADQQ AHEAKIKL
Length:338
Mass (Da):38,044
Last modified:June 13, 2006 - v1
Checksum:iBCEEBBC0BCA12C6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573326 Genomic DNA. Translation: CAK17475.1.
RefSeqiWP_011535837.1. NC_008027.1.

Genome annotation databases

EnsemblBacteriaiCAK17475; CAK17475; PSEEN4819.
GeneIDi32807778.
KEGGipen:PSEEN4819.

Similar proteinsi

Entry informationi

Entry nameiLIPA_PSEE4
AccessioniPrimary (citable) accession number: Q1I4G1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 13, 2006
Last modified: November 22, 2017
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families