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Q1I4G1

- LIPA_PSEE4

UniProt

Q1I4G1 - LIPA_PSEE4

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Protein

Lipoyl synthase

Gene
lipA, PSEEN4819
Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi110 – 1101Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi114 – 1141Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi117 – 1171Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPENT384676:GJB8-4581-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:PSEEN4819
OrganismiPseudomonas entomophila (strain L48)
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000658: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Lipoyl synthaseUniRule annotationPRO_1000012256Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi384676.PSEEN4819.

Structurei

3D structure databases

ProteinModelPortaliQ1I4G1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1I4G1-1 [UniParc]FASTAAdd to Basket

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MTTVQEAVPN LIPTQDVTPR PAPKKVEAGV KLRGAEKVAR IPVKIIPTEE    50
LPKKPDWIRV RIPVSPEVDR IKQLLRKHKL HSVCEEASCP NLGECFSGGT 100
ATFMIMGDIC TRRCPFCDVG HGRPKPLDLD EPKNLAIAIA DLRLKYVVIT 150
SVDRDDLRDG GAQHFADCIR EIRALSPGVQ LETLVPDYRG RMDVALEITA 200
QTPPDVFNHN LETVPRLYKA ARPGSDYDWS LDLLQKFKQM VPHVPTKSGL 250
MLGLGETDEE VIEVMHRMRE HDIDMLTLGQ YLQPSRNHLP VQRFVHPDTF 300
AWFAEEGYKM GFKNVASGPL VRSSYHADQQ AHEAKIKL 338
Length:338
Mass (Da):38,044
Last modified:June 13, 2006 - v1
Checksum:iBCEEBBC0BCA12C6A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT573326 Genomic DNA. Translation: CAK17475.1.
RefSeqiYP_610258.1. NC_008027.1.

Genome annotation databases

EnsemblBacteriaiCAK17475; CAK17475; PSEEN4819.
GeneIDi4089518.
KEGGipen:PSEEN4819.
PATRICi19867646. VBIPseEnt83862_4620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT573326 Genomic DNA. Translation: CAK17475.1 .
RefSeqi YP_610258.1. NC_008027.1.

3D structure databases

ProteinModelPortali Q1I4G1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 384676.PSEEN4819.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAK17475 ; CAK17475 ; PSEEN4819 .
GeneIDi 4089518.
KEGGi pen:PSEEN4819.
PATRICi 19867646. VBIPseEnt83862_4620.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci PENT384676:GJB8-4581-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the entomopathogenic and metabolically versatile soil bacterium Pseudomonas entomophila."
    Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C., Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P., Weissenbach J., Lemaitre B., Medigue C., Boccard F.
    Nat. Biotechnol. 24:673-679(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: L48.

Entry informationi

Entry nameiLIPA_PSEE4
AccessioniPrimary (citable) accession number: Q1I4G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 13, 2006
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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