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Protein

Biotin synthase

Gene

bioB

Organism
Pseudomonas entomophila (strain L48)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi63Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi66Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi103Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi134Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi194Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi266Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:PSEEN5121
OrganismiPseudomonas entomophila (strain L48)
Taxonomic identifieri384676 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000658 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003815561 – 352Biotin synthaseAdd BLAST352

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi384676.PSEEN5121.

Structurei

3D structure databases

ProteinModelPortaliQ1I3N6.
SMRiQ1I3N6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF. Bacteria.
COG0502. LUCA.
HOGENOMiHOG000239957.
KOiK01012.
OMAiADRFCMG.
OrthoDBiPOG091H01DF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR034416. BATS_domain_containing.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
PANTHERiPTHR22976. PTHR22976. 1 hit.
PfamiView protein in Pfam
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SFLDiSFLDF00272. biotin_synthase. 1 hit.
SFLDG01060. BATS_domain_containing. 1 hit.
SFLDG01278. biotin_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1I3N6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASAIATTR HDWSLAEVKA LFQQPFNDLL FQAQTMHRAY FDPNRVQVST
60 70 80 90 100
LLSIKTGACP EDCKYCPQSG HYNTGLEKQK LMEVQKVLEE AARAKAIGST
110 120 130 140 150
RFCMGAAWKH PSAKDMPYVL EMVKGVKAMG LETCMTLGKL DQEQTLALAQ
160 170 180 190 200
AGLDYYNHNL DTSPEFYGSI ITTRTYSERL QTLAYVRDAG MKICSGGILG
210 220 230 240 250
MGESLDDRAG LLIQLANLPE HPESVPINML VKVAGTPLAE EEDVDPFDFI
260 270 280 290 300
RMLAVARILM PKSHVRLSAG REQMNEQMQA LAFMAGANSI FYGEKLLTTA
310 320 330 340 350
NPQADKDMQL FARLGIKPEA REEHADEVHQ AAIEQALVEQ RNSELFYDAA

SA
Length:352
Mass (Da):38,987
Last modified:June 13, 2006 - v1
Checksum:iAD29FF9508BB04CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573326 Genomic DNA. Translation: CAK17750.1.
RefSeqiWP_011536109.1. NC_008027.1.

Genome annotation databases

EnsemblBacteriaiCAK17750; CAK17750; PSEEN5121.
GeneIDi32808054.
KEGGipen:PSEEN5121.

Similar proteinsi

Entry informationi

Entry nameiBIOB_PSEE4
AccessioniPrimary (citable) accession number: Q1I3N6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 13, 2006
Last modified: November 22, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families