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Q1I2B5

- Q1I2B5_9INFA

UniProt

Q1I2B5 - Q1I2B5_9INFA

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Protein
Submitted name: Nucleocapsid protein
Gene
NP
Organism
Influenza A virus (A/WSN/1933 TS61(H1N1))
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.SAAS annotations

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural molecule activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. viral penetration into host nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interactionSAAS annotations, Viral penetration into host nucleusSAAS annotations, Virus entry into host cell

Keywords - Ligandi

RNA-bindingSAAS annotations, Viral nucleoproteinImported

Names & Taxonomyi

Protein namesi
Submitted name:
Nucleocapsid proteinImported
Gene namesi
Name:NPImported
OrganismiInfluenza A virus (A/WSN/1933 TS61(H1N1))Imported
Taxonomic identifieri370129 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A

Subcellular locationi

Virion. Host nucleus By similarity SAAS annotations

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-SubCell
  3. intracellular Source: GOC
  4. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid proteinSAAS annotations, Host nucleusSAAS annotations, Virion

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.

Protein-protein interaction databases

DIPiDIP-60419N.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IQHX-ray3.20A/B/C8-498[»]
2YMNelectron microscopy20.00A/B/C/D/E/F1-498[»]

Miscellaneous databases

EvolutionaryTraceiQ1I2B5.

Family & Domainsi

Family and domain databases

InterProiIPR002141. Flu_NP.
[Graphical view]
PfamiPF00506. Flu_NP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1I2B5-1 [UniParc]FASTAAdd to Basket

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MATKGTKRSY EQMETDGERQ NATEIRASVG KMIDGIGRFY IQMCTELKLS    50
DYEGRLIQNS LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYRRV 100
DGKWRRELIL YDKEEIRRIW RQANNGDDAT AGLTHMMIWH SNLNDATYQR 150
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG 200
INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQRTMVD QVRESRNPGN 250
AEFEDLIFLA RSALILRGSV AHKSCLPACV YGSAVASGYD FEREGYSLVG 300
IDPFRLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT 350
KVVPRGKLST RGVQIASNEN METMESSTLE LRSRYWAIRT RSGGNTNQQR 400
ASSGQISIQP TFSVQRNLPF DRPTIMAAFT GNTEGRTSDM RTEIIRLMES 450
ARPEDVSFQG RGVFELSDEK ATSPIVPSFD MSNEGSYFFG DNAEEYDN 498
Length:498
Mass (Da):56,377
Last modified:June 13, 2006 - v1
Checksum:i91390B2963EAB5A3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CY010791 Genomic RNA. Translation: ABF47959.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CY010791 Genomic RNA. Translation: ABF47959.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IQH X-ray 3.20 A/B/C 8-498 [» ]
2YMN electron microscopy 20.00 A/B/C/D/E/F 1-498 [» ]
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-60419N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q1I2B5.

Family and domain databases

InterProi IPR002141. Flu_NP.
[Graphical view ]
Pfami PF00506. Flu_NP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA."
    Ye Q., Krug R.M., Tao Y.J.
    Nature 444:1078-1082(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) OF 8-498.
  2. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: A/WSN/1933 TS61Imported.
  3. The NIAID Influenza Genome Sequencing Consortium
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: A/WSN/1933 TS61Imported.
  4. "Organization of the influenza virus replication machinery."
    Moeller A., Kirchdoerfer R.N., Potter C.S., Carragher B., Wilson I.A.
    Science 338:1631-1634(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (20.00 ANGSTROMS).

Entry informationi

Entry nameiQ1I2B5_9INFA
AccessioniPrimary (citable) accession number: Q1I2B5
Entry historyi
Integrated into UniProtKB/TrEMBL: June 13, 2006
Last sequence update: June 13, 2006
Last modified: February 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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