Q1I2B5 (Q1I2B5_9INFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Nucleocapsid protein EMBL ABF47959.1

Gene names

Name:

NP EMBL ABF47959.1

Organism

Influenza A virus (A/WSN/1933 TS61(H1N1)) EMBL ABF47959.1

Taxonomic identifier

370129 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype › Influenza A virus (A/WSN/1933(H1N1))

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity. SAAS SAAS002141

Subunit structure

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity. SAAS SAAS002141

Ontologies

Keywords
Biological process	Host-virus interaction SAAS SAAS002141 Viral penetration into host nucleus SAAS SAAS002141 Virus entry into host cell
Cellular component	Host nucleus SAAS SAAS002141 Virion
Ligand	RNA-binding SAAS SAAS002141 Viral nucleoprotein EMBL ABF47959.1
Technical term	3D-structure PDB 2YMN PDB 2IQH
Gene Ontology (GO)
Biological_process	viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral penetration into host nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-KW virion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q1I2B5 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 91390B2963EAB5A3	FASTA	498	56,377
	10 20 30 40 50 60 MATKGTKRSY EQMETDGERQ NATEIRASVG KMIDGIGRFY IQMCTELKLS DYEGRLIQNS 70 80 90 100 110 120 LTIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYRRV DGKWRRELIL YDKEEIRRIW 130 140 150 160 170 180 RQANNGDDAT AGLTHMMIWH SNLNDATYQR TRALVRTGMD PRMCSLMQGS TLPRRSGAAG 190 200 210 220 230 240 AAVKGVGTMV MELIRMIKRG INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQRTMVD 250 260 270 280 290 300 QVRESRNPGN AEFEDLIFLA RSALILRGSV AHKSCLPACV YGSAVASGYD FEREGYSLVG 310 320 330 340 350 360 IDPFRLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT KVVPRGKLST 370 380 390 400 410 420 RGVQIASNEN METMESSTLE LRSRYWAIRT RSGGNTNQQR ASSGQISIQP TFSVQRNLPF 430 440 450 460 470 480 DRPTIMAAFT GNTEGRTSDM RTEIIRLMES ARPEDVSFQG RGVFELSDEK ATSPIVPSFD 490 MSNEGSYFFG DNAEEYDN

References

[1]	"The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA." Ye Q., Krug R.M., Tao Y.J. Nature 444:1078-1082(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) OF 8-498.
[2]	"The NIAID Influenza Genome Sequencing Project." Spiro D., Ghedin E., Sengamalay N., Halpin R., Boyne A., Zaborsky J., Feldblyum T., Subbu V., Sparenborg J., Shumway M., Sitz J., Katzel D., Koo H., Salzberg S.L., Griesemer S., St George K., Bennett R., Taylor J. , Bennink J.R., Yewdell J.W., Bao Y., Bolotov P., Dernovoy D., Kiryutin B., Lipman D.J., Tatusova T. Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: A/WSN/1933 TS61 EMBL ABF47959.1.
[3]	The NIAID Influenza Genome Sequencing Consortium Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: A/WSN/1933 TS61 EMBL ABF47959.1.
[4]	"Organization of the influenza virus replication machinery." Moeller A., Kirchdoerfer R.N., Potter C.S., Carragher B., Wilson I.A. Science 338:1631-1634(2012) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (20.00 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CY010791 Genomic RNA. Translation: ABF47959.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IQH	X-ray	3.20	A/B/C	8-498	[»]
2YMN	electron microscopy	20.00	A/B/C/D/E/F	1-498	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR002141. Flu_NP.
[Graphical view]

Pfam

PF00506. Flu_NP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q1I2B5.

Entry information

Entry name

Q1I2B5_9INFA

Accession

Primary (citable) accession number: Q1I2B5

Entry history

Integrated into UniProtKB/TrEMBL:	June 13, 2006
Last sequence update:	June 13, 2006
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)