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Q1HVH9

- DEN_EBVA8

UniProt

Q1HVH9 - DEN_EBVA8

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Protein
Deneddylase
Gene
BPLF1
Organism
Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei48 – 481Important for catalytic activity By similarity
Active sitei61 – 611 By similarity
Active sitei193 – 1931 By similarity
Active sitei195 – 1951 By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Large tegument protein
Short name:
LTP
Gene namesi
ORF Names:BPLF1
OrganismiEpstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri82830 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007639: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31543154Deneddylase
PRO_0000375969Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Protein-protein interaction databases

MINTiMINT-6768255.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 258218Peptidase C76
Add
BLAST
Repeati335 – 33951
Repeati340 – 34452
Repeati345 – 34953
Repeati350 – 35454
Repeati355 – 35955
Repeati360 – 36456
Repeati365 – 36957
Repeati370 – 37458
Repeati375 – 37959

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 269269Deubiquitination activity By similarity
Add
BLAST
Regioni335 – 379459 X 5 AA repeats of P-A-S-A-A
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1105 – 113531 Reviewed prediction
Add
BLAST
Coiled coili1399 – 144345 Reviewed prediction
Add
BLAST
Coiled coili1711 – 174030 Reviewed prediction
Add
BLAST
Coiled coili1880 – 190930 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi417 – 4237Nuclear localization signal By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 38146Ala-rich
Add
BLAST
Compositional biasi532 – 5354Poly-Ala
Compositional biasi1361 – 13644Poly-Asn
Compositional biasi1480 – 14889Poly-Ala
Compositional biasi1563 – 15686Poly-Gly
Compositional biasi1661 – 16644Poly-Pro
Compositional biasi1835 – 18384Poly-Ala
Compositional biasi2090 – 20934Poly-Arg
Compositional biasi2105 – 21084Poly-Ala
Compositional biasi2850 – 2953104Ala-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1HVH9-1 [UniParc]FASTAAdd to Basket

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MSNGDWGQSQ RTRGTGPVRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH     50
CKFGRFAGIQ CVSNCVLYLV KSFLAGRPLT SRPELDEVLD EGARLDALMR 100
QSGILKGHEM AQLTDVPSSV VLRGGGRVHI YRSAEIFGLV LFPAQIANSA 150
VVQSLAEVLH GSYNGVAQFI LYICDIYAGA IIIETDGSFY LFDPHCQKDA 200
APGTPAHVRV STYAHDILQY VGAPGAQYTC VHLYFLPEAF ETEDPRIFML 250
EHYGVYDFYE ANGSGFDLVG PELVSSDGEA AGTPGADSSP PVMLPFERRI 300
IPYNLRPLPS RSFTSDSFPA ARYSPAKTNS PPSSPASAAP ASAAPASAAP 350
ASAAPASAAP ASAAPASAAP ASAAPASAAP ASSPPLFIPI PGLGHTPGVP 400
APSTPPRASG GAAPQTPKRK KGLGKDSPHK KPTSGRRLPL SSTTDTEDDQ 450
LPRTHVPPHR PPSAARLPPP VIPIPHQSPP ASPTPRPAPV STIAPSVTPS 500
PRLPLQIPIP LPQAAPSNPE IPLTTPSPSP TAAAAPTATT LSPPPTQQQP 550
PQSAAPAPSP PPTQQQPPQS AAPAPSPLLP QQQPPPSAAR APSPLPPQQQ 600
PLPSATPAPP HAQQLPPSAT TLEPEKNNPS AADRAGTEIS PSPPFGQQPS 650
FGDDASGGSG LVRYLSDLEE PFLSMSDSEE AESDLASDIP TTEDEDMFED 700
EVFSNSLESG SSAPTSPITL DTARSQYYQT TFDIETPEMD FVPLESNIAR 750
IAGHTYQEQA IVYDPASNRE VPEADALSMI DYLLVTVVLE QGLIRSRDRS 800
AVLNLLEFLK DWSGHLQVPT LDLEQLLTSE LNIQNLANML SENKGRAGEF 850
HEHLAAKLEA CLPSLATKDA VRVDAGAKML AEIPQLAESG DGKFDLEAAR 900
RRLTDLLSGG DQEGEEGGGE PEDHSIYRGP HVDVPLVLDD ESWKRLLSLA 950
EAARTAVARQ QAGVDEEDVR FLALLTAIEY GAPPAASVPP FVHNVPVRSK 1000
NAALHVRRCT ADIRDKVASA ASDYLSYLED PSLPTVMDFD DLLTHLRHTC 1050
QIIASLPLLN IRYTSIEWDY RELLYLGTAL SDMSGIPWPL ERVEEDDPSI 1100
APLPEFETVA KKQKELETTR ENEKRLRTIL DDIEAMLGLA GVASAPGAPI 1150
SPASPSATPA NHDNPEATPP LADTAALTIP VIEKYIANAG SIVGAAKNPT 1200
YIRLRDTIQQ IVRSKKYLMN ILKSITFYTI DNYIASFEES IDHLYRDLPV 1250
LDPEVQDGID RILDPMVSEA LHTFEMGNRL TLEPARLVAL QNFATHSTLK 1300
ETAAAVNLLP GLLAVYDATV TGQAPEDALR LLSGLQNQLS QTLIPGKLKK 1350
RFLSYLQKLK NNNNDQLRQK EVQAWRLEAE GFKPATEEQL EAFLDTAPNK 1400
ELKRQYEKKL RQLMETGRKE KEKLREQEDK ERRERRAREA NEAWARIRKA 1450
LGARPEPAPT SPDDWNTLLA SLLPDNTDSA AAAAAAVARN TDILDSLTQI 1500
LAAMLLGITR VRRERLRSLL VDDGGAAERM EAVEPGWFTE IETGPLARLD 1550
AWPATPAATA KEGGGGRGAE EAAGALFRAR TAADAIRSAL AQTRQALQSP 1600
DMKSAVVNTD LEAPYAEYER GLAGLLEKRR SAEAALTAIV SEYVDRTLPE 1650
ATNDPGQANL PPPPTIPQAT APPRLASDSA LWPKKPQLLT RRERDDLLQA 1700
TGDFFSELLT EAEAAEVRAL EEQVRESQTL MAKAHEMAAS TRRGFHTALE 1750
AVLSRSRDEA PDDELRSLLP SPPKAPVQAP LEAALARAAA GNGSWPYRKS 1800
LAAAKWIRGI CEAVRGLSEG ALALAGGVGA WLNLAAAADG EIHELTRLLE 1850
VEGMAQNSMD GMEELRLALA TLDPKRVAGG KETVADWKRR LSRLEAIIQE 1900
AQEESQLQGT LQDLVTQARG HTDPRQLKIV VEAARGLALG ASAGSQYALL 1950
KDKLLRYASA KQSFLAFYET AQPTVFVKHP LTNNLPLLIT ISAPPTGWGN 2000
GAPTRRAQFL AAAGPAKYAG TLWLETESPC DPLNPAYVSA DTQEPLNYIP 2050
VYHNFLEYVM PTVLENPEAF SLTPAGRPQA IGPPQDDQER RRRTLASVAS 2100
ARLSAAAADS YWDTWPDVES NAGELLREYV SAPKALMEDL ADNPIVAMTL 2150
LAHASLIASR NHPPYPAPAT DREVILLEQR EMMALLVGTH PAYAAAFLGA 2200
PSFYAGLGLV SALARDGGLG DLLSDSVLTY RLVRSPASGR GGMPSTTRGS 2250
NDGEDARRLT RHRIAGPPTG FIFFQDAWEE MDTRAALWPH PEFLGLVHNQ 2300
STARARACML LLARRCFAPE ALQQLWHSLR PLEGPVAFQD YLRDFVKQAY 2350
TRGEELPRAE GLEVPRETPS SYGTVTGRAL RNLMPYGTPI TGPKRGSGDT 2400
IPVSVFEAAV AAAFLGRPLT LFVSSQYLFN LKTLGQVRVV APLLYCDGHS 2450
EPFRSLVETI SLNFLQDLDG YSESFEPEMS IFARQAVWLR ELLTEARAAK 2500
PKEARPPTVA ILANRKNIIW KCFTYRHNLP DVQFYFNAAG ASRWPTDVLN 2550
PSFYEHEDPP LPVGYQLPPN PRNVQELFSG FPPRVGHGLV SGDGFQSADN 2600
TPASSDRLQQ LGGGETDQGE EGSTTAESEA SGPPSPQSPL LEKVAPGRPR 2650
DWLSPTSSPR DVTVTPGLAA PITLPGPRLM ARPYFGAETR ASESPDRSPG 2700
TSPRPWPKDS LELLPQPAPQ QPPSSPWASE QGPIVYTLSP NSTPSTASGS 2750
QKKHTIQIPG LVPSQKPSYP PSAPYKPGQS TGGIAPTPSA ASLTTFGLQP 2800
QDTQASSQDP PYGHSIMQRE KKQQGGREEA AEIRPSATRL PTAVGLRPRA 2850
PVVAAGAAAS ATPAFDPGEA PSGFPIPQAP ALGSGLAAPA HTPVGALAPR 2900
PQKTQAQRPQ DAAALPTPTI KAVGARPVPK ATGALAAGAR PRGQPTAAPP 2950
SAASPPRVSL PVRSRQQQSP AIPLPPMHSG SEPGARPEVR LSQYRHAGPQ 3000
TYTVRKEAPP SAASQLPKMP KCKDSMYYPP SGSARYPAPF QALSFSQSVA 3050
SPAPSSDQTT LLWNTPSVVT QFLSIEDIIR EVVTGGSTSG DLVVPSGSPS 3100
SLSTAAPEQD LRYSLTLSQA SRVLSRFVSQ LRRKLERSTH RLIADLERLK 3150
FLYL 3154
Length:3,154
Mass (Da):338,449
Last modified:June 13, 2006 - v1
Checksum:iBFD719F4742849CB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ279927 Genomic DNA. Translation: ABB89229.1.
RefSeqiYP_001129449.1. NC_009334.1.

Genome annotation databases

GeneIDi5176165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ279927 Genomic DNA. Translation: ABB89229.1 .
RefSeqi YP_001129449.1. NC_009334.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

MINTi MINT-6768255.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5176165.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Epstein-Barr virus type 2 strain AG876."
    Dolan A., Addison C., Gatherer D., Davison A.J., McGeoch D.J.
    Virology 350:164-170(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_EBVA8
AccessioniPrimary (citable) accession number: Q1HVH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 13, 2006
Last modified: May 14, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi