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Q1HVH9 (DEN_EBVA8) Reviewed, UniProtKB/Swiss-Prot

Last modified June 26, 2013. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Large tegument protein
Short name=LTP
Gene names
ORF Names:BPLF1
OrganismEpstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4) [Reference proteome]
Taxonomic identifier82830 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length3154 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument. Host cytoplasm. Host nucleus By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 31543154Deneddylase
PRO_0000375969

Regions

Domain41 – 258218Peptidase C76
Repeat335 – 33951
Repeat340 – 34452
Repeat345 – 34953
Repeat350 – 35454
Repeat355 – 35955
Repeat360 – 36456
Repeat365 – 36957
Repeat370 – 37458
Repeat375 – 37959
Region1 – 269269Deubiquitination activity By similarity
Region335 – 379459 X 5 AA repeats of P-A-S-A-A
Coiled coil1105 – 113531 Potential
Coiled coil1399 – 144345 Potential
Coiled coil1711 – 174030 Potential
Coiled coil1880 – 190930 Potential
Motif417 – 4237Nuclear localization signal By similarity
Compositional bias336 – 38146Ala-rich
Compositional bias532 – 5354Poly-Ala
Compositional bias1361 – 13644Poly-Asn
Compositional bias1480 – 14889Poly-Ala
Compositional bias1563 – 15686Poly-Gly
Compositional bias1661 – 16644Poly-Pro
Compositional bias1835 – 18384Poly-Ala
Compositional bias2090 – 20934Poly-Arg
Compositional bias2105 – 21084Poly-Ala
Compositional bias2850 – 2953104Ala-rich

Sites

Active site611 By similarity
Active site1931 By similarity
Active site1951 By similarity
Site481Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1HVH9 [UniParc].

Last modified June 13, 2006. Version 1.
Checksum: BFD719F4742849CB

FASTA3,154338,449
        10         20         30         40         50         60 
MSNGDWGQSQ RTRGTGPVRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH CKFGRFAGIQ 

        70         80         90        100        110        120 
CVSNCVLYLV KSFLAGRPLT SRPELDEVLD EGARLDALMR QSGILKGHEM AQLTDVPSSV 

       130        140        150        160        170        180 
VLRGGGRVHI YRSAEIFGLV LFPAQIANSA VVQSLAEVLH GSYNGVAQFI LYICDIYAGA 

       190        200        210        220        230        240 
IIIETDGSFY LFDPHCQKDA APGTPAHVRV STYAHDILQY VGAPGAQYTC VHLYFLPEAF 

       250        260        270        280        290        300 
ETEDPRIFML EHYGVYDFYE ANGSGFDLVG PELVSSDGEA AGTPGADSSP PVMLPFERRI 

       310        320        330        340        350        360 
IPYNLRPLPS RSFTSDSFPA ARYSPAKTNS PPSSPASAAP ASAAPASAAP ASAAPASAAP 

       370        380        390        400        410        420 
ASAAPASAAP ASAAPASAAP ASSPPLFIPI PGLGHTPGVP APSTPPRASG GAAPQTPKRK 

       430        440        450        460        470        480 
KGLGKDSPHK KPTSGRRLPL SSTTDTEDDQ LPRTHVPPHR PPSAARLPPP VIPIPHQSPP 

       490        500        510        520        530        540 
ASPTPRPAPV STIAPSVTPS PRLPLQIPIP LPQAAPSNPE IPLTTPSPSP TAAAAPTATT 

       550        560        570        580        590        600 
LSPPPTQQQP PQSAAPAPSP PPTQQQPPQS AAPAPSPLLP QQQPPPSAAR APSPLPPQQQ 

       610        620        630        640        650        660 
PLPSATPAPP HAQQLPPSAT TLEPEKNNPS AADRAGTEIS PSPPFGQQPS FGDDASGGSG 

       670        680        690        700        710        720 
LVRYLSDLEE PFLSMSDSEE AESDLASDIP TTEDEDMFED EVFSNSLESG SSAPTSPITL 

       730        740        750        760        770        780 
DTARSQYYQT TFDIETPEMD FVPLESNIAR IAGHTYQEQA IVYDPASNRE VPEADALSMI 

       790        800        810        820        830        840 
DYLLVTVVLE QGLIRSRDRS AVLNLLEFLK DWSGHLQVPT LDLEQLLTSE LNIQNLANML 

       850        860        870        880        890        900 
SENKGRAGEF HEHLAAKLEA CLPSLATKDA VRVDAGAKML AEIPQLAESG DGKFDLEAAR 

       910        920        930        940        950        960 
RRLTDLLSGG DQEGEEGGGE PEDHSIYRGP HVDVPLVLDD ESWKRLLSLA EAARTAVARQ 

       970        980        990       1000       1010       1020 
QAGVDEEDVR FLALLTAIEY GAPPAASVPP FVHNVPVRSK NAALHVRRCT ADIRDKVASA 

      1030       1040       1050       1060       1070       1080 
ASDYLSYLED PSLPTVMDFD DLLTHLRHTC QIIASLPLLN IRYTSIEWDY RELLYLGTAL 

      1090       1100       1110       1120       1130       1140 
SDMSGIPWPL ERVEEDDPSI APLPEFETVA KKQKELETTR ENEKRLRTIL DDIEAMLGLA 

      1150       1160       1170       1180       1190       1200 
GVASAPGAPI SPASPSATPA NHDNPEATPP LADTAALTIP VIEKYIANAG SIVGAAKNPT 

      1210       1220       1230       1240       1250       1260 
YIRLRDTIQQ IVRSKKYLMN ILKSITFYTI DNYIASFEES IDHLYRDLPV LDPEVQDGID 

      1270       1280       1290       1300       1310       1320 
RILDPMVSEA LHTFEMGNRL TLEPARLVAL QNFATHSTLK ETAAAVNLLP GLLAVYDATV 

      1330       1340       1350       1360       1370       1380 
TGQAPEDALR LLSGLQNQLS QTLIPGKLKK RFLSYLQKLK NNNNDQLRQK EVQAWRLEAE 

      1390       1400       1410       1420       1430       1440 
GFKPATEEQL EAFLDTAPNK ELKRQYEKKL RQLMETGRKE KEKLREQEDK ERRERRAREA 

      1450       1460       1470       1480       1490       1500 
NEAWARIRKA LGARPEPAPT SPDDWNTLLA SLLPDNTDSA AAAAAAVARN TDILDSLTQI 

      1510       1520       1530       1540       1550       1560 
LAAMLLGITR VRRERLRSLL VDDGGAAERM EAVEPGWFTE IETGPLARLD AWPATPAATA 

      1570       1580       1590       1600       1610       1620 
KEGGGGRGAE EAAGALFRAR TAADAIRSAL AQTRQALQSP DMKSAVVNTD LEAPYAEYER 

      1630       1640       1650       1660       1670       1680 
GLAGLLEKRR SAEAALTAIV SEYVDRTLPE ATNDPGQANL PPPPTIPQAT APPRLASDSA 

      1690       1700       1710       1720       1730       1740 
LWPKKPQLLT RRERDDLLQA TGDFFSELLT EAEAAEVRAL EEQVRESQTL MAKAHEMAAS 

      1750       1760       1770       1780       1790       1800 
TRRGFHTALE AVLSRSRDEA PDDELRSLLP SPPKAPVQAP LEAALARAAA GNGSWPYRKS 

      1810       1820       1830       1840       1850       1860 
LAAAKWIRGI CEAVRGLSEG ALALAGGVGA WLNLAAAADG EIHELTRLLE VEGMAQNSMD 

      1870       1880       1890       1900       1910       1920 
GMEELRLALA TLDPKRVAGG KETVADWKRR LSRLEAIIQE AQEESQLQGT LQDLVTQARG 

      1930       1940       1950       1960       1970       1980 
HTDPRQLKIV VEAARGLALG ASAGSQYALL KDKLLRYASA KQSFLAFYET AQPTVFVKHP 

      1990       2000       2010       2020       2030       2040 
LTNNLPLLIT ISAPPTGWGN GAPTRRAQFL AAAGPAKYAG TLWLETESPC DPLNPAYVSA 

      2050       2060       2070       2080       2090       2100 
DTQEPLNYIP VYHNFLEYVM PTVLENPEAF SLTPAGRPQA IGPPQDDQER RRRTLASVAS 

      2110       2120       2130       2140       2150       2160 
ARLSAAAADS YWDTWPDVES NAGELLREYV SAPKALMEDL ADNPIVAMTL LAHASLIASR 

      2170       2180       2190       2200       2210       2220 
NHPPYPAPAT DREVILLEQR EMMALLVGTH PAYAAAFLGA PSFYAGLGLV SALARDGGLG 

      2230       2240       2250       2260       2270       2280 
DLLSDSVLTY RLVRSPASGR GGMPSTTRGS NDGEDARRLT RHRIAGPPTG FIFFQDAWEE 

      2290       2300       2310       2320       2330       2340 
MDTRAALWPH PEFLGLVHNQ STARARACML LLARRCFAPE ALQQLWHSLR PLEGPVAFQD 

      2350       2360       2370       2380       2390       2400 
YLRDFVKQAY TRGEELPRAE GLEVPRETPS SYGTVTGRAL RNLMPYGTPI TGPKRGSGDT 

      2410       2420       2430       2440       2450       2460 
IPVSVFEAAV AAAFLGRPLT LFVSSQYLFN LKTLGQVRVV APLLYCDGHS EPFRSLVETI 

      2470       2480       2490       2500       2510       2520 
SLNFLQDLDG YSESFEPEMS IFARQAVWLR ELLTEARAAK PKEARPPTVA ILANRKNIIW 

      2530       2540       2550       2560       2570       2580 
KCFTYRHNLP DVQFYFNAAG ASRWPTDVLN PSFYEHEDPP LPVGYQLPPN PRNVQELFSG 

      2590       2600       2610       2620       2630       2640 
FPPRVGHGLV SGDGFQSADN TPASSDRLQQ LGGGETDQGE EGSTTAESEA SGPPSPQSPL 

      2650       2660       2670       2680       2690       2700 
LEKVAPGRPR DWLSPTSSPR DVTVTPGLAA PITLPGPRLM ARPYFGAETR ASESPDRSPG 

      2710       2720       2730       2740       2750       2760 
TSPRPWPKDS LELLPQPAPQ QPPSSPWASE QGPIVYTLSP NSTPSTASGS QKKHTIQIPG 

      2770       2780       2790       2800       2810       2820 
LVPSQKPSYP PSAPYKPGQS TGGIAPTPSA ASLTTFGLQP QDTQASSQDP PYGHSIMQRE 

      2830       2840       2850       2860       2870       2880 
KKQQGGREEA AEIRPSATRL PTAVGLRPRA PVVAAGAAAS ATPAFDPGEA PSGFPIPQAP 

      2890       2900       2910       2920       2930       2940 
ALGSGLAAPA HTPVGALAPR PQKTQAQRPQ DAAALPTPTI KAVGARPVPK ATGALAAGAR 

      2950       2960       2970       2980       2990       3000 
PRGQPTAAPP SAASPPRVSL PVRSRQQQSP AIPLPPMHSG SEPGARPEVR LSQYRHAGPQ 

      3010       3020       3030       3040       3050       3060 
TYTVRKEAPP SAASQLPKMP KCKDSMYYPP SGSARYPAPF QALSFSQSVA SPAPSSDQTT 

      3070       3080       3090       3100       3110       3120 
LLWNTPSVVT QFLSIEDIIR EVVTGGSTSG DLVVPSGSPS SLSTAAPEQD LRYSLTLSQA 

      3130       3140       3150 
SRVLSRFVSQ LRRKLERSTH RLIADLERLK FLYL 

« Hide

References

[1]"The genome of Epstein-Barr virus type 2 strain AG876."
Dolan A., Addison C., Gatherer D., Davison A.J., McGeoch D.J.
Virology 350:164-170(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ279927 Genomic DNA. Translation: ABB89229.1.
RefSeqYP_001129449.1. NC_009334.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-6768255.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5176165.

Phylogenomic databases

ProtClustDBCLSP2512080.

Family and domain databases

InterProIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_EBVA8
AccessionPrimary (citable) accession number: Q1HVH9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 13, 2006
Last modified: June 26, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries