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Q1HVH9

- DEN_EBVA8

UniProt

Q1HVH9 - DEN_EBVA8

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Protein

Deneddylase

Gene

BPLF1

Organism
Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei48 – 481Important for catalytic activityPROSITE-ProRule annotation
Active sitei61 – 611PROSITE-ProRule annotation
Active sitei193 – 1931PROSITE-ProRule annotation
Active sitei195 – 1951PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Large tegument protein
Short name:
LTP
Gene namesi
ORF Names:BPLF1
OrganismiEpstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri82830 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007639: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31543154DeneddylasePRO_0000375969Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Protein-protein interaction databases

MINTiMINT-6768255.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 258218Peptidase C76PROSITE-ProRule annotationAdd
BLAST
Repeati335 – 33951
Repeati340 – 34452
Repeati345 – 34953
Repeati350 – 35454
Repeati355 – 35955
Repeati360 – 36456
Repeati365 – 36957
Repeati370 – 37458
Repeati375 – 37959

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 269269Deubiquitination activityBy similarityAdd
BLAST
Regioni335 – 379459 X 5 AA repeats of P-A-S-A-AAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1105 – 113531Sequence AnalysisAdd
BLAST
Coiled coili1399 – 144345Sequence AnalysisAdd
BLAST
Coiled coili1711 – 174030Sequence AnalysisAdd
BLAST
Coiled coili1880 – 190930Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi417 – 4237Nuclear localization signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 38146Ala-richAdd
BLAST
Compositional biasi532 – 5354Poly-Ala
Compositional biasi1361 – 13644Poly-Asn
Compositional biasi1480 – 14889Poly-Ala
Compositional biasi1563 – 15686Poly-Gly
Compositional biasi1661 – 16644Poly-Pro
Compositional biasi1835 – 18384Poly-Ala
Compositional biasi2090 – 20934Poly-Arg
Compositional biasi2105 – 21084Poly-Ala
Compositional biasi2850 – 2953104Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1HVH9-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSNGDWGQSQ RTRGTGPVRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH
60 70 80 90 100
CKFGRFAGIQ CVSNCVLYLV KSFLAGRPLT SRPELDEVLD EGARLDALMR
110 120 130 140 150
QSGILKGHEM AQLTDVPSSV VLRGGGRVHI YRSAEIFGLV LFPAQIANSA
160 170 180 190 200
VVQSLAEVLH GSYNGVAQFI LYICDIYAGA IIIETDGSFY LFDPHCQKDA
210 220 230 240 250
APGTPAHVRV STYAHDILQY VGAPGAQYTC VHLYFLPEAF ETEDPRIFML
260 270 280 290 300
EHYGVYDFYE ANGSGFDLVG PELVSSDGEA AGTPGADSSP PVMLPFERRI
310 320 330 340 350
IPYNLRPLPS RSFTSDSFPA ARYSPAKTNS PPSSPASAAP ASAAPASAAP
360 370 380 390 400
ASAAPASAAP ASAAPASAAP ASAAPASAAP ASSPPLFIPI PGLGHTPGVP
410 420 430 440 450
APSTPPRASG GAAPQTPKRK KGLGKDSPHK KPTSGRRLPL SSTTDTEDDQ
460 470 480 490 500
LPRTHVPPHR PPSAARLPPP VIPIPHQSPP ASPTPRPAPV STIAPSVTPS
510 520 530 540 550
PRLPLQIPIP LPQAAPSNPE IPLTTPSPSP TAAAAPTATT LSPPPTQQQP
560 570 580 590 600
PQSAAPAPSP PPTQQQPPQS AAPAPSPLLP QQQPPPSAAR APSPLPPQQQ
610 620 630 640 650
PLPSATPAPP HAQQLPPSAT TLEPEKNNPS AADRAGTEIS PSPPFGQQPS
660 670 680 690 700
FGDDASGGSG LVRYLSDLEE PFLSMSDSEE AESDLASDIP TTEDEDMFED
710 720 730 740 750
EVFSNSLESG SSAPTSPITL DTARSQYYQT TFDIETPEMD FVPLESNIAR
760 770 780 790 800
IAGHTYQEQA IVYDPASNRE VPEADALSMI DYLLVTVVLE QGLIRSRDRS
810 820 830 840 850
AVLNLLEFLK DWSGHLQVPT LDLEQLLTSE LNIQNLANML SENKGRAGEF
860 870 880 890 900
HEHLAAKLEA CLPSLATKDA VRVDAGAKML AEIPQLAESG DGKFDLEAAR
910 920 930 940 950
RRLTDLLSGG DQEGEEGGGE PEDHSIYRGP HVDVPLVLDD ESWKRLLSLA
960 970 980 990 1000
EAARTAVARQ QAGVDEEDVR FLALLTAIEY GAPPAASVPP FVHNVPVRSK
1010 1020 1030 1040 1050
NAALHVRRCT ADIRDKVASA ASDYLSYLED PSLPTVMDFD DLLTHLRHTC
1060 1070 1080 1090 1100
QIIASLPLLN IRYTSIEWDY RELLYLGTAL SDMSGIPWPL ERVEEDDPSI
1110 1120 1130 1140 1150
APLPEFETVA KKQKELETTR ENEKRLRTIL DDIEAMLGLA GVASAPGAPI
1160 1170 1180 1190 1200
SPASPSATPA NHDNPEATPP LADTAALTIP VIEKYIANAG SIVGAAKNPT
1210 1220 1230 1240 1250
YIRLRDTIQQ IVRSKKYLMN ILKSITFYTI DNYIASFEES IDHLYRDLPV
1260 1270 1280 1290 1300
LDPEVQDGID RILDPMVSEA LHTFEMGNRL TLEPARLVAL QNFATHSTLK
1310 1320 1330 1340 1350
ETAAAVNLLP GLLAVYDATV TGQAPEDALR LLSGLQNQLS QTLIPGKLKK
1360 1370 1380 1390 1400
RFLSYLQKLK NNNNDQLRQK EVQAWRLEAE GFKPATEEQL EAFLDTAPNK
1410 1420 1430 1440 1450
ELKRQYEKKL RQLMETGRKE KEKLREQEDK ERRERRAREA NEAWARIRKA
1460 1470 1480 1490 1500
LGARPEPAPT SPDDWNTLLA SLLPDNTDSA AAAAAAVARN TDILDSLTQI
1510 1520 1530 1540 1550
LAAMLLGITR VRRERLRSLL VDDGGAAERM EAVEPGWFTE IETGPLARLD
1560 1570 1580 1590 1600
AWPATPAATA KEGGGGRGAE EAAGALFRAR TAADAIRSAL AQTRQALQSP
1610 1620 1630 1640 1650
DMKSAVVNTD LEAPYAEYER GLAGLLEKRR SAEAALTAIV SEYVDRTLPE
1660 1670 1680 1690 1700
ATNDPGQANL PPPPTIPQAT APPRLASDSA LWPKKPQLLT RRERDDLLQA
1710 1720 1730 1740 1750
TGDFFSELLT EAEAAEVRAL EEQVRESQTL MAKAHEMAAS TRRGFHTALE
1760 1770 1780 1790 1800
AVLSRSRDEA PDDELRSLLP SPPKAPVQAP LEAALARAAA GNGSWPYRKS
1810 1820 1830 1840 1850
LAAAKWIRGI CEAVRGLSEG ALALAGGVGA WLNLAAAADG EIHELTRLLE
1860 1870 1880 1890 1900
VEGMAQNSMD GMEELRLALA TLDPKRVAGG KETVADWKRR LSRLEAIIQE
1910 1920 1930 1940 1950
AQEESQLQGT LQDLVTQARG HTDPRQLKIV VEAARGLALG ASAGSQYALL
1960 1970 1980 1990 2000
KDKLLRYASA KQSFLAFYET AQPTVFVKHP LTNNLPLLIT ISAPPTGWGN
2010 2020 2030 2040 2050
GAPTRRAQFL AAAGPAKYAG TLWLETESPC DPLNPAYVSA DTQEPLNYIP
2060 2070 2080 2090 2100
VYHNFLEYVM PTVLENPEAF SLTPAGRPQA IGPPQDDQER RRRTLASVAS
2110 2120 2130 2140 2150
ARLSAAAADS YWDTWPDVES NAGELLREYV SAPKALMEDL ADNPIVAMTL
2160 2170 2180 2190 2200
LAHASLIASR NHPPYPAPAT DREVILLEQR EMMALLVGTH PAYAAAFLGA
2210 2220 2230 2240 2250
PSFYAGLGLV SALARDGGLG DLLSDSVLTY RLVRSPASGR GGMPSTTRGS
2260 2270 2280 2290 2300
NDGEDARRLT RHRIAGPPTG FIFFQDAWEE MDTRAALWPH PEFLGLVHNQ
2310 2320 2330 2340 2350
STARARACML LLARRCFAPE ALQQLWHSLR PLEGPVAFQD YLRDFVKQAY
2360 2370 2380 2390 2400
TRGEELPRAE GLEVPRETPS SYGTVTGRAL RNLMPYGTPI TGPKRGSGDT
2410 2420 2430 2440 2450
IPVSVFEAAV AAAFLGRPLT LFVSSQYLFN LKTLGQVRVV APLLYCDGHS
2460 2470 2480 2490 2500
EPFRSLVETI SLNFLQDLDG YSESFEPEMS IFARQAVWLR ELLTEARAAK
2510 2520 2530 2540 2550
PKEARPPTVA ILANRKNIIW KCFTYRHNLP DVQFYFNAAG ASRWPTDVLN
2560 2570 2580 2590 2600
PSFYEHEDPP LPVGYQLPPN PRNVQELFSG FPPRVGHGLV SGDGFQSADN
2610 2620 2630 2640 2650
TPASSDRLQQ LGGGETDQGE EGSTTAESEA SGPPSPQSPL LEKVAPGRPR
2660 2670 2680 2690 2700
DWLSPTSSPR DVTVTPGLAA PITLPGPRLM ARPYFGAETR ASESPDRSPG
2710 2720 2730 2740 2750
TSPRPWPKDS LELLPQPAPQ QPPSSPWASE QGPIVYTLSP NSTPSTASGS
2760 2770 2780 2790 2800
QKKHTIQIPG LVPSQKPSYP PSAPYKPGQS TGGIAPTPSA ASLTTFGLQP
2810 2820 2830 2840 2850
QDTQASSQDP PYGHSIMQRE KKQQGGREEA AEIRPSATRL PTAVGLRPRA
2860 2870 2880 2890 2900
PVVAAGAAAS ATPAFDPGEA PSGFPIPQAP ALGSGLAAPA HTPVGALAPR
2910 2920 2930 2940 2950
PQKTQAQRPQ DAAALPTPTI KAVGARPVPK ATGALAAGAR PRGQPTAAPP
2960 2970 2980 2990 3000
SAASPPRVSL PVRSRQQQSP AIPLPPMHSG SEPGARPEVR LSQYRHAGPQ
3010 3020 3030 3040 3050
TYTVRKEAPP SAASQLPKMP KCKDSMYYPP SGSARYPAPF QALSFSQSVA
3060 3070 3080 3090 3100
SPAPSSDQTT LLWNTPSVVT QFLSIEDIIR EVVTGGSTSG DLVVPSGSPS
3110 3120 3130 3140 3150
SLSTAAPEQD LRYSLTLSQA SRVLSRFVSQ LRRKLERSTH RLIADLERLK

FLYL
Length:3,154
Mass (Da):338,449
Last modified:June 13, 2006 - v1
Checksum:iBFD719F4742849CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ279927 Genomic DNA. Translation: ABB89229.1.
RefSeqiYP_001129449.1. NC_009334.1.

Genome annotation databases

GeneIDi5176165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ279927 Genomic DNA. Translation: ABB89229.1 .
RefSeqi YP_001129449.1. NC_009334.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-6768255.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5176165.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Epstein-Barr virus type 2 strain AG876."
    Dolan A., Addison C., Gatherer D., Davison A.J., McGeoch D.J.
    Virology 350:164-170(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_EBVA8
AccessioniPrimary (citable) accession number: Q1HVH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 13, 2006
Last modified: October 29, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3