Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q1HVH9

- DEN_EBVA8

UniProt

Q1HVH9 - DEN_EBVA8

Protein

Deneddylase

Gene

BPLF1

Organism
Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 31 (01 Oct 2014)
      Sequence version 1 (13 Jun 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei48 – 481Important for catalytic activityPROSITE-ProRule annotation
    Active sitei61 – 611PROSITE-ProRule annotation
    Active sitei193 – 1931PROSITE-ProRule annotation
    Active sitei195 – 1951PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Large tegument protein
    Short name:
    LTP
    Gene namesi
    ORF Names:BPLF1
    OrganismiEpstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4)
    Taxonomic identifieri82830 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007639: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 31543154DeneddylasePRO_0000375969Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Protein-protein interaction databases

    MINTiMINT-6768255.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 258218Peptidase C76PROSITE-ProRule annotationAdd
    BLAST
    Repeati335 – 33951
    Repeati340 – 34452
    Repeati345 – 34953
    Repeati350 – 35454
    Repeati355 – 35955
    Repeati360 – 36456
    Repeati365 – 36957
    Repeati370 – 37458
    Repeati375 – 37959

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 269269Deubiquitination activityBy similarityAdd
    BLAST
    Regioni335 – 379459 X 5 AA repeats of P-A-S-A-AAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1105 – 113531Sequence AnalysisAdd
    BLAST
    Coiled coili1399 – 144345Sequence AnalysisAdd
    BLAST
    Coiled coili1711 – 174030Sequence AnalysisAdd
    BLAST
    Coiled coili1880 – 190930Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi417 – 4237Nuclear localization signalBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi336 – 38146Ala-richAdd
    BLAST
    Compositional biasi532 – 5354Poly-Ala
    Compositional biasi1361 – 13644Poly-Asn
    Compositional biasi1480 – 14889Poly-Ala
    Compositional biasi1563 – 15686Poly-Gly
    Compositional biasi1661 – 16644Poly-Pro
    Compositional biasi1835 – 18384Poly-Ala
    Compositional biasi2090 – 20934Poly-Arg
    Compositional biasi2105 – 21084Poly-Ala
    Compositional biasi2850 – 2953104Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q1HVH9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNGDWGQSQ RTRGTGPVRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH     50
    CKFGRFAGIQ CVSNCVLYLV KSFLAGRPLT SRPELDEVLD EGARLDALMR 100
    QSGILKGHEM AQLTDVPSSV VLRGGGRVHI YRSAEIFGLV LFPAQIANSA 150
    VVQSLAEVLH GSYNGVAQFI LYICDIYAGA IIIETDGSFY LFDPHCQKDA 200
    APGTPAHVRV STYAHDILQY VGAPGAQYTC VHLYFLPEAF ETEDPRIFML 250
    EHYGVYDFYE ANGSGFDLVG PELVSSDGEA AGTPGADSSP PVMLPFERRI 300
    IPYNLRPLPS RSFTSDSFPA ARYSPAKTNS PPSSPASAAP ASAAPASAAP 350
    ASAAPASAAP ASAAPASAAP ASAAPASAAP ASSPPLFIPI PGLGHTPGVP 400
    APSTPPRASG GAAPQTPKRK KGLGKDSPHK KPTSGRRLPL SSTTDTEDDQ 450
    LPRTHVPPHR PPSAARLPPP VIPIPHQSPP ASPTPRPAPV STIAPSVTPS 500
    PRLPLQIPIP LPQAAPSNPE IPLTTPSPSP TAAAAPTATT LSPPPTQQQP 550
    PQSAAPAPSP PPTQQQPPQS AAPAPSPLLP QQQPPPSAAR APSPLPPQQQ 600
    PLPSATPAPP HAQQLPPSAT TLEPEKNNPS AADRAGTEIS PSPPFGQQPS 650
    FGDDASGGSG LVRYLSDLEE PFLSMSDSEE AESDLASDIP TTEDEDMFED 700
    EVFSNSLESG SSAPTSPITL DTARSQYYQT TFDIETPEMD FVPLESNIAR 750
    IAGHTYQEQA IVYDPASNRE VPEADALSMI DYLLVTVVLE QGLIRSRDRS 800
    AVLNLLEFLK DWSGHLQVPT LDLEQLLTSE LNIQNLANML SENKGRAGEF 850
    HEHLAAKLEA CLPSLATKDA VRVDAGAKML AEIPQLAESG DGKFDLEAAR 900
    RRLTDLLSGG DQEGEEGGGE PEDHSIYRGP HVDVPLVLDD ESWKRLLSLA 950
    EAARTAVARQ QAGVDEEDVR FLALLTAIEY GAPPAASVPP FVHNVPVRSK 1000
    NAALHVRRCT ADIRDKVASA ASDYLSYLED PSLPTVMDFD DLLTHLRHTC 1050
    QIIASLPLLN IRYTSIEWDY RELLYLGTAL SDMSGIPWPL ERVEEDDPSI 1100
    APLPEFETVA KKQKELETTR ENEKRLRTIL DDIEAMLGLA GVASAPGAPI 1150
    SPASPSATPA NHDNPEATPP LADTAALTIP VIEKYIANAG SIVGAAKNPT 1200
    YIRLRDTIQQ IVRSKKYLMN ILKSITFYTI DNYIASFEES IDHLYRDLPV 1250
    LDPEVQDGID RILDPMVSEA LHTFEMGNRL TLEPARLVAL QNFATHSTLK 1300
    ETAAAVNLLP GLLAVYDATV TGQAPEDALR LLSGLQNQLS QTLIPGKLKK 1350
    RFLSYLQKLK NNNNDQLRQK EVQAWRLEAE GFKPATEEQL EAFLDTAPNK 1400
    ELKRQYEKKL RQLMETGRKE KEKLREQEDK ERRERRAREA NEAWARIRKA 1450
    LGARPEPAPT SPDDWNTLLA SLLPDNTDSA AAAAAAVARN TDILDSLTQI 1500
    LAAMLLGITR VRRERLRSLL VDDGGAAERM EAVEPGWFTE IETGPLARLD 1550
    AWPATPAATA KEGGGGRGAE EAAGALFRAR TAADAIRSAL AQTRQALQSP 1600
    DMKSAVVNTD LEAPYAEYER GLAGLLEKRR SAEAALTAIV SEYVDRTLPE 1650
    ATNDPGQANL PPPPTIPQAT APPRLASDSA LWPKKPQLLT RRERDDLLQA 1700
    TGDFFSELLT EAEAAEVRAL EEQVRESQTL MAKAHEMAAS TRRGFHTALE 1750
    AVLSRSRDEA PDDELRSLLP SPPKAPVQAP LEAALARAAA GNGSWPYRKS 1800
    LAAAKWIRGI CEAVRGLSEG ALALAGGVGA WLNLAAAADG EIHELTRLLE 1850
    VEGMAQNSMD GMEELRLALA TLDPKRVAGG KETVADWKRR LSRLEAIIQE 1900
    AQEESQLQGT LQDLVTQARG HTDPRQLKIV VEAARGLALG ASAGSQYALL 1950
    KDKLLRYASA KQSFLAFYET AQPTVFVKHP LTNNLPLLIT ISAPPTGWGN 2000
    GAPTRRAQFL AAAGPAKYAG TLWLETESPC DPLNPAYVSA DTQEPLNYIP 2050
    VYHNFLEYVM PTVLENPEAF SLTPAGRPQA IGPPQDDQER RRRTLASVAS 2100
    ARLSAAAADS YWDTWPDVES NAGELLREYV SAPKALMEDL ADNPIVAMTL 2150
    LAHASLIASR NHPPYPAPAT DREVILLEQR EMMALLVGTH PAYAAAFLGA 2200
    PSFYAGLGLV SALARDGGLG DLLSDSVLTY RLVRSPASGR GGMPSTTRGS 2250
    NDGEDARRLT RHRIAGPPTG FIFFQDAWEE MDTRAALWPH PEFLGLVHNQ 2300
    STARARACML LLARRCFAPE ALQQLWHSLR PLEGPVAFQD YLRDFVKQAY 2350
    TRGEELPRAE GLEVPRETPS SYGTVTGRAL RNLMPYGTPI TGPKRGSGDT 2400
    IPVSVFEAAV AAAFLGRPLT LFVSSQYLFN LKTLGQVRVV APLLYCDGHS 2450
    EPFRSLVETI SLNFLQDLDG YSESFEPEMS IFARQAVWLR ELLTEARAAK 2500
    PKEARPPTVA ILANRKNIIW KCFTYRHNLP DVQFYFNAAG ASRWPTDVLN 2550
    PSFYEHEDPP LPVGYQLPPN PRNVQELFSG FPPRVGHGLV SGDGFQSADN 2600
    TPASSDRLQQ LGGGETDQGE EGSTTAESEA SGPPSPQSPL LEKVAPGRPR 2650
    DWLSPTSSPR DVTVTPGLAA PITLPGPRLM ARPYFGAETR ASESPDRSPG 2700
    TSPRPWPKDS LELLPQPAPQ QPPSSPWASE QGPIVYTLSP NSTPSTASGS 2750
    QKKHTIQIPG LVPSQKPSYP PSAPYKPGQS TGGIAPTPSA ASLTTFGLQP 2800
    QDTQASSQDP PYGHSIMQRE KKQQGGREEA AEIRPSATRL PTAVGLRPRA 2850
    PVVAAGAAAS ATPAFDPGEA PSGFPIPQAP ALGSGLAAPA HTPVGALAPR 2900
    PQKTQAQRPQ DAAALPTPTI KAVGARPVPK ATGALAAGAR PRGQPTAAPP 2950
    SAASPPRVSL PVRSRQQQSP AIPLPPMHSG SEPGARPEVR LSQYRHAGPQ 3000
    TYTVRKEAPP SAASQLPKMP KCKDSMYYPP SGSARYPAPF QALSFSQSVA 3050
    SPAPSSDQTT LLWNTPSVVT QFLSIEDIIR EVVTGGSTSG DLVVPSGSPS 3100
    SLSTAAPEQD LRYSLTLSQA SRVLSRFVSQ LRRKLERSTH RLIADLERLK 3150
    FLYL 3154
    Length:3,154
    Mass (Da):338,449
    Last modified:June 13, 2006 - v1
    Checksum:iBFD719F4742849CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ279927 Genomic DNA. Translation: ABB89229.1.
    RefSeqiYP_001129449.1. NC_009334.1.

    Genome annotation databases

    GeneIDi5176165.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ279927 Genomic DNA. Translation: ABB89229.1 .
    RefSeqi YP_001129449.1. NC_009334.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-6768255.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5176165.

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Epstein-Barr virus type 2 strain AG876."
      Dolan A., Addison C., Gatherer D., Davison A.J., McGeoch D.J.
      Virology 350:164-170(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiDEN_EBVA8
    AccessioniPrimary (citable) accession number: Q1HVH9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3