Q1HPU2 (TRMB_BOMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: tRNA (guanine-N(7)-)-methyltransferase EC=2.1.1.33 Alternative name(s): tRNA (guanine(46)-N(7))-methyltransferase tRNA(m7G46)-methyltransferase
Organism	Bombyx mori (Silk moth) [Reference proteome]
Taxonomic identifier	7091 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Amphiesmenoptera › Lepidoptera › Glossata › Neolepidoptera › Heteroneura › Ditrysia › Obtectomera › Bombycoidea › Bombyciformes › Bombycidae › Bombycinae › Bombyx

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370566
Regions
	Region	77 – 78	2	S-adenosyl-L-methionine binding By similarity
	Region	110 – 111	2	S-adenosyl-L-methionine binding By similarity
	Region	208 – 210	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	133	1	By similarity
	Binding site	54	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	130	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1HPU2 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 33A20A8B8D556ECE	FASTA	236	27,802
	10 20 30 40 50 60 MALPQKKYYR QRAHSNPIAD HCFDYPSHPD DYDWTPLYPI ISEKPNQVEF LDVGCGYGGL 70 80 90 100 110 120 LVALSPMFPS NLMLGSEIRV KVSDYVNDRI KALRVQYPDQ YQNVAVLRTN AMKYLPNFFH 130 140 150 160 170 180 KGQLKKMFFL YPDPHFKKAK HKWRIINKWL LSEYAYVLCE QGIVYTITDV KDLNEWMVAH 190 200 210 220 230 FEEHPLFESV PEEELKEDPI IEKLYESTEE GQKVTRNNGE KFLAVFRRIA DKTKTN

References

[1]	"Blast silkworm EST database for functional genes." Niu B.L., Meng Z.Q., Weng H.B., Shen W.F., He L.H., Zheng K.F., Ye S.T., Lin T.B., Chen J.E. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ443310 mRNA. Translation: ABF51399.1.
RefSeq	NP_001040444.1. NM_001046979.1.
UniGene	Bmo.7070.
3D structure databases
ProteinModelPortal	Q1HPU2.
SMR	Q1HPU2. Positions 25-231.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	732983.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_BOMMO

Accession

Primary (citable) accession number: Q1HPU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	June 13, 2006
Last modified:	October 3, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)