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Q1HPU2 (TRMB_BOMMO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
OrganismBombyx mori (Silk moth) [Reference proteome]
Taxonomic identifier7091 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaBombycoideaBombycidaeBombycinaeBombyx

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 236236tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370566

Regions

Region77 – 782S-adenosyl-L-methionine binding By similarity
Region110 – 1112S-adenosyl-L-methionine binding By similarity
Region208 – 2103S-adenosyl-L-methionine binding By similarity

Sites

Active site1331 By similarity
Binding site541S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1301S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1HPU2 [UniParc].

Last modified June 13, 2006. Version 1.
Checksum: 33A20A8B8D556ECE

FASTA23627,802
        10         20         30         40         50         60 
MALPQKKYYR QRAHSNPIAD HCFDYPSHPD DYDWTPLYPI ISEKPNQVEF LDVGCGYGGL 

        70         80         90        100        110        120 
LVALSPMFPS NLMLGSEIRV KVSDYVNDRI KALRVQYPDQ YQNVAVLRTN AMKYLPNFFH 

       130        140        150        160        170        180 
KGQLKKMFFL YPDPHFKKAK HKWRIINKWL LSEYAYVLCE QGIVYTITDV KDLNEWMVAH 

       190        200        210        220        230 
FEEHPLFESV PEEELKEDPI IEKLYESTEE GQKVTRNNGE KFLAVFRRIA DKTKTN 

« Hide

References

[1]"Blast silkworm EST database for functional genes."
Niu B.L., Meng Z.Q., Weng H.B., Shen W.F., He L.H., Zheng K.F., Ye S.T., Lin T.B., Chen J.E.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ443310 mRNA. Translation: ABF51399.1.
RefSeqNP_001040444.1. NM_001046979.1.
UniGeneBmo.7070.

3D structure databases

ProteinModelPortalQ1HPU2.
SMRQ1HPU2. Positions 25-231.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID732983.
KEGGbmor:732983.

Phylogenomic databases

KOK03439.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BOMMO
AccessionPrimary (citable) accession number: Q1HPU2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 13, 2006
Last modified: April 16, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways