Q1HKZ5 (M3K13_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase kinase kinase 13
EC=2.7.11.25

Gene names

Name:

Map3k13

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	959 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by autophosphorylation and homodimerization By similarity.
Subunit structure	Homodimer; forms dimers through the leucine-zipper motif. Interacts with the C-terminus of MAPK8IP1 through the kinase catalytic domain. Binds PRDX3. Associates with the IKK complex through the kinase domain.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein By similarity.
Post-translational modification	Autophosphorylated on serine and threonine residues By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	JNK cascade Inferred from electronic annotation. Source: Compara positive regulation of NF-kappaB transcription factor activity Inferred from electronic annotation. Source: Compara protein autophosphorylation Inferred from electronic annotation. Source: Compara
Cellular_component	IkappaB kinase complex Inferred from electronic annotation. Source: Compara membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 959	959	Mitogen-activated protein kinase kinase kinase 13		PRO_0000277591
Regions
	Domain	167 – 408	242	Protein kinase
	Nucleotide binding	173 – 181	9	ATP By similarity
	Region	432 – 453	22	Leucine-zipper 1
	Region	485 – 506	22	Leucine-zipper 2
Sites
	Active site	278	1	Proton acceptor By similarity
	Binding site	194	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1HKZ5 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: 18424BA1695DEDAE	FASTA	959	106,987
	10 20 30 40 50 60 MANPQEHLSC SSLPHLPLTE NKTSGGRNEL AAMGNHPSPK LPEDPQERGA IQSELMEITG 70 80 90 100 110 120 SPISTTVLTS VSEDSRGQFE NSVLQLREQD ESEMTLSLGN SNTVDGENTN GPEDIKIQFS 130 140 150 160 170 180 RSGSGSGGFL EGLFGCLRPV WNIIGKAYST DYKLQQQDTW EVPFEEISEL QWLGSGAQGA 190 200 210 220 230 240 VFLGKFRAEE VAIKKVREQN ETDIKHLRKL KHPNIIAFKG VCTQAPCYCI IMEYCAHGQL 250 260 270 280 290 300 YEVLRAGRKI TPRLLVDWST GIASGMNYLH LHKIIHRDLK SPNVLVTHTD AVKISDFGTS 310 320 330 340 350 360 KELSDKSTKM SFAGTVAWMA PEVIRNEPVS EKVDIWSFGV VLWELLTGEI PYKDVDSSAI 370 380 390 400 410 420 IWGVGSNSLH LPVPSTCPDG FKILMKQTWQ SKPRNRPSFR QTLMHLDIAS ADVLATPQET 430 440 450 460 470 480 YFKSQAEWRE EVKKHFEKIK SEGTCIHRLD EELIRRRREE LRHALDIREH YERKLERANN 490 500 510 520 530 540 LYMELSAIML QLEMREKELL KREQAVEKKY PGTYKRHPVR PIIHPNAMEK LMKRKGVPHK 550 560 570 580 590 600 AGVQTKRPDL LRSEGIPSTE AVPTASPLSG SPKMSTASSR SRYRSKPRHR RGNSRGSHSD 610 620 630 640 650 660 FAAILKTQPA QENSPHPTYM HHTQAQCASV HQHNPLQQQY QQIPPAQPQS RHPRLNAHGQ 670 680 690 700 710 720 DIATCANNLR YFGPAAALRS PLSNHAQRQM PGSSPDLIST AMAADWRNSE LDQDQVGPWG 730 740 750 760 770 780 CCQAEPYDPC FQCRPEHSGS LDVPTTEPVG RSPDLSSSPA HNPLSGNAQG SERTGANGFS 790 800 810 820 830 840 GCQSGISHQF TPPMLPQKTR PLQKSGDDSS EEEGEVDSEV EFPRRQRPHR CISSYQSYST 850 860 870 880 890 900 FSSENFSVSD GEEGNTSDHS NSPDESANRR QDRLAETLDD LLSQTPEAPI EISSHSDGLS 910 920 930 940 950 DKECAVRRVK TQMSLGKLCA EERGYENPVQ FGDSDCDSSE GECSDATVRT SKNYSSATW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Itoh A., Itoh T. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 690-959. Strain: C57BL/6J. Tissue: Spinal ganglion.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ480427 mRNA. Translation: ABF19581.1. AK051378 mRNA. Translation: BAC34618.1.
IPI	IPI00132644.
RefSeq	NP_766409.2. NM_172821.3.
UniGene	Mm.420837. Mm.438136.
3D structure databases
ProteinModelPortal	Q1HKZ5.
SMR	Q1HKZ5. Positions 111-504.
ModBase	Search...
PTM databases
PhosphoSite	Q1HKZ5.
Proteomic databases
PaxDb	Q1HKZ5.
PRIDE	Q1HKZ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000042065; ENSMUSP00000047388; ENSMUSG00000033618.
GeneID	71751.
KEGG	mmu:71751.
UCSC	uc007yrr.2. mouse.
Organism-specific databases
CTD	9175.
MGI	MGI:2444243. Map3k13.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00680000099647.
HOGENOM	HOG000113435.
HOVERGEN	HBG052383.
InParanoid	Q1HKZ5.
KO	K04422.
OMA	HRRGNSR.
OrthoDB	EOG45MN4T.
Gene expression databases
Bgee	Q1HKZ5.
CleanEx	MM_MAP3K13.
Genevestigator	Q1HKZ5.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR017419. MAP3K12_MAP3K13. IPR027258. MAPKKK13. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PIRSF	PIRSF038165. MAPKKK12_MAPKKK13. 1 hit. PIRSF500742. MAPKKK13. 1 hit.
PRINTS	PR00109. TYRKINASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	334401.
SOURCE	Search...

Entry information

Entry name

M3K13_MOUSE

Accession

Primary (citable) accession number: Q1HKZ5
Secondary accession number(s): Q8BKN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	June 13, 2006
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program