Reviewed, UniProtKB/Swiss-Prot Q1HG70 (MP2K2_CANFA)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 2
      Short name=MAP kinase kinase 2
      Short name=MAPKK 2
    EC=2.7.12.2
Alternative name(s):
    ERK activator kinase 2
    MAPK/ERK kinase 2
      Short name=MEK2

Gene names

Name:	MAP2K2
Synonyms:	MEK2

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with MORG1 By similarity.
Post-translational modification	MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1) By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Dual specificity mitogen-activated protein kinase kinase 2		PRO_0000289624
Regions
	Domain	72 – 369	298	Protein kinase
	Nucleotide binding	78 – 86	9	ATP By similarity
	Compositional bias	266 – 315	50	Pro-rich
Sites
	Active site	194	1	Proton acceptor By similarity
	Binding site	101	1	ATP By similarity
	Site	10 – 11	2	Cleavage; by anthrax lethal factor By similarity
Amino acid modifications
	Modified residue	23	1	Phosphoserine By similarity
	Modified residue	222	1	Phosphoserine; by RAF By similarity
	Modified residue	226	1	Phosphoserine By similarity
	Modified residue	293	1	Phosphoserine By similarity
	Modified residue	295	1	Phosphoserine By similarity
	Modified residue	394	1	Phosphothreonine By similarity
	Modified residue	396	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1HG70-1 [UniParc]. Last modified June 13, 2006. Version 1. Checksum: E783573AE967EB44	FASTA	400	44,446
	10 20 30 40 50 60 MLARRKPVLP ALTINPAIAE GPSPTSEGAS EANLVDLQKK LAELELDEQQ KKRLEAFLTQ 70 80 90 100 110 120 KAKVGELKDD DFERISELGA GNGGVVTKVQ HRPSGLIMAR KLIHLEIKPA IRNQIIRELQ 130 140 150 160 170 180 VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEEIL GKVSIAVLRG 190 200 210 220 230 240 LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ 250 260 270 280 290 300 GTHYSVQSDI WSMGLSLVEL SIGRYPIPPP DAKELEAIFG RPMVDGIEGE PHSISPRPRP 310 320 330 340 350 360 PGRPISGHGT DSRPAMAIFE LLDYIVNEPP PKLPNGVFTQ DFQEFVNKCL IKNPAERADL 370 380 390 400 KMLMSHTFIK RSEVEEVDFA GWLCKTLRLN QPSTPTRTAV

References

[1]	"Constitutive activation of MEK1/2 in an invasive variant of MSV-transformed MDCK cell line." Guerard K.-P., Noel J. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ489531 mRNA. Translation: ABF47220.1.
RefSeq	NP_001041601.1.
UniGene	Cfa.21225
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q1HG70.
Genome annotation databases
Ensembl	ENSCAFT00000030400; ENSCAFP00000028245; ENSCAFG00000019138; Canis familiaris. [Genome view]
GeneID	611939.
KEGG	cfa:611939.
Organism-specific databases
CTD	611939.
Phylogenomic databases
HOVERGEN	Q1HG70.
Enzyme and pathway databases
BRENDA	2.7.12.2. 463.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MP2K2_CANFA

Accession

Primary (citable) accession number: Q1HG70

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	June 13, 2006
Last modified:	October 13, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)