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Protein

Dual specificity mitogen-activated protein kinase kinase 2

Gene

MAP2K2

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011ATPPROSITE-ProRule annotation
Active sitei194 – 1941Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 869ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CFA-112411. MAPK1 (ERK2) activation.
R-CFA-445144. Signal transduction by L1.
R-CFA-5673000. RAF activation.
R-CFA-5674135. MAP2K and MAPK activation.
R-CFA-5674499. Negative feedback regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity mitogen-activated protein kinase kinase 2 (EC:2.7.12.2By similarity)
Short name:
MAP kinase kinase 2
Short name:
MAPKK 2
Alternative name(s):
ERK activator kinase 2
MAPK/ERK kinase 2
Short name:
MEK 2
Gene namesi
Name:MAP2K2
Synonyms:MEK2
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 20

Subcellular locationi

  • Cytoplasm By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity

  • Note: Membrane localization is probably regulated by its interaction with KSR1.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Dual specificity mitogen-activated protein kinase kinase 2PRO_0000289624Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei23 – 231PhosphoserineBy similarity
Modified residuei222 – 2221Phosphoserine; by RAFBy similarity
Modified residuei226 – 2261PhosphoserineBy similarity
Modified residuei293 – 2931PhosphoserineBy similarity
Modified residuei295 – 2951PhosphoserineBy similarity
Modified residuei306 – 3061PhosphoserineBy similarity
Modified residuei394 – 3941PhosphothreonineBy similarity
Modified residuei396 – 3961PhosphothreonineBy similarity

Post-translational modificationi

MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 112Cleavage; by anthrax lethal factorBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ1HG70.

Interactioni

Subunit structurei

Interacts with MORG1, SGK1 and KSR1.By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000028245.

Structurei

3D structure databases

ProteinModelPortaliQ1HG70.
SMRiQ1HG70. Positions 60-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 369298Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi266 – 31550Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
HOVERGENiHBG108518.
InParanoidiQ1HG70.
KOiK04369.
OMAiEGASEAH.
OrthoDBiEOG091G0DEC.
TreeFamiTF105137.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q1HG70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLARRKPVLP ALTINPAIAE GPSPTSEGAS EANLVDLQKK LAELELDEQQ
60 70 80 90 100
KKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKVQ HRPSGLIMAR
110 120 130 140 150
KLIHLEIKPA IRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM
160 170 180 190 200
DGGSLDQVLK EAKRIPEEIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI
210 220 230 240 250
LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI
260 270 280 290 300
WSMGLSLVEL SIGRYPIPPP DAKELEAIFG RPMVDGIEGE PHSISPRPRP
310 320 330 340 350
PGRPISGHGT DSRPAMAIFE LLDYIVNEPP PKLPNGVFTQ DFQEFVNKCL
360 370 380 390 400
IKNPAERADL KMLMSHTFIK RSEVEEVDFA GWLCKTLRLN QPSTPTRTAV
Length:400
Mass (Da):44,446
Last modified:June 13, 2006 - v1
Checksum:iE783573AE967EB44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ489531 mRNA. Translation: ABF47220.1.
RefSeqiNP_001041601.1. NM_001048136.1.
UniGeneiCfa.21225.

Genome annotation databases

EnsembliENSCAFT00000030400; ENSCAFP00000028245; ENSCAFG00000019138.
GeneIDi611939.
KEGGicfa:611939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ489531 mRNA. Translation: ABF47220.1.
RefSeqiNP_001041601.1. NM_001048136.1.
UniGeneiCfa.21225.

3D structure databases

ProteinModelPortaliQ1HG70.
SMRiQ1HG70. Positions 60-392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000028245.

Proteomic databases

PaxDbiQ1HG70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000030400; ENSCAFP00000028245; ENSCAFG00000019138.
GeneIDi611939.
KEGGicfa:611939.

Organism-specific databases

CTDi5605.

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
GeneTreeiENSGT00760000119199.
HOGENOMiHOG000234206.
HOVERGENiHBG108518.
InParanoidiQ1HG70.
KOiK04369.
OMAiEGASEAH.
OrthoDBiEOG091G0DEC.
TreeFamiTF105137.

Enzyme and pathway databases

ReactomeiR-CFA-112411. MAPK1 (ERK2) activation.
R-CFA-445144. Signal transduction by L1.
R-CFA-5673000. RAF activation.
R-CFA-5674135. MAP2K and MAPK activation.
R-CFA-5674499. Negative feedback regulation of MAPK pathway.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMP2K2_CANLF
AccessioniPrimary (citable) accession number: Q1HG70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 13, 2006
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.