Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP-dependent DNA helicase PIF1

Gene

Pif1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi224 – 2318ATPUniRule annotation
DNA bindingi573 – 59220UniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase PIF1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
DNA repair and recombination helicase PIF1UniRule annotation
PIF1/RRM3 DNA helicase-like protein
Gene namesi
Name:Pif1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1586053. Pif1.

Subcellular locationi

  • Nucleus UniRule annotation
  • Mitochondrion UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637ATP-dependent DNA helicase PIF1PRO_0000295092Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271PhosphoserineBy similarity
Modified residuei151 – 1511PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ1HG60.
PRIDEiQ1HG60.

Expressioni

Gene expression databases

BgeeiENSRNOG00000015878.

Interactioni

Subunit structurei

Monomer. Interacts with telomerase.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021265.

Structurei

3D structure databases

ProteinModelPortaliQ1HG60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 180180PINTBy similarityAdd
BLAST

Domaini

The PIF1 N-terminal (PINT) domain enhances the interaction with ssDNA through intrinsic binding activity, it also harbors DNA strand-annealing activity.By similarity

Sequence similaritiesi

Belongs to the helicase family. PIF1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0987. Eukaryota.
COG0507. LUCA.
GeneTreeiENSGT00530000063561.
HOGENOMiHOG000132960.
HOVERGENiHBG106706.
InParanoidiQ1HG60.
KOiK15255.
OMAiRACTSGK.
OrthoDBiEOG091G03MY.
PhylomeDBiQ1HG60.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_03176. PIF1. 1 hit.
InterProiIPR003840. DNA_helicase.
IPR010285. DNA_helicase_pif1-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23274. PTHR23274. 1 hit.
PfamiPF02689. Herpes_Helicase. 1 hit.
PF05970. PIF1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequencei

Sequence statusi: Complete.

Q1HG60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSSTEVATD ECDDTELRCR VAVEELSPGG QPRKRQALRA AELSLGRNER
60 70 80 90 100
RELMLRLQAP GPEGRPRCFP LRAVRLFTRF AAVGRSTLRL PADGVPRAGS
110 120 130 140 150
VQLLLSDCPP ERLRRFLRTL RLKLAVAPGP GPASARAQLL GPRPRDFVTI
160 170 180 190 200
SPVQPEELRR AAATKAPDSA LEKRPMESQP SMEAPRWPLP VKKLRMPSSK
210 220 230 240 250
PKLSEEQAAV LRMVLKGQSI FFTGSAGTGK SYLLKHILGS LPPTGTVATA
260 270 280 290 300
STGVAACHIG GTTLHAFAGI GSGQAPLAQC VALAHRPGVR QGWLNCQRLV
310 320 330 340 350
IDEISMVEAD FFDKLEAVAR AVRQQKKPFG GIQLIICGDF LQLPPVTKGS
360 370 380 390 400
QHPRFCFQAK SWRKCVPVTL ELTEVWRQAD QTFISLLKAV RLGRCSDEVT
410 420 430 440 450
RQLRATAAHK VGRDGIIATR LCTHQDDVAL TNEKRLKELP GDVHSFEAID
460 470 480 490 500
SDPELSRTLD AQCPVGRVLQ LKLGAQVMLV KNLAVSRGLV NGARGVVVGF
510 520 530 540 550
ESEGRGLPRV RFLCGITEVI RTDRWTVQVT GGQYLSRQQL PLQLAWAMSI
560 570 580 590 600
HKSQGMSLDC VEISLGRVFA SGQAYVALSR ARSLQGLRVL DFDPTVVRCD
610 620 630
SRVLQFYATL RQGRGLSLES QDDEEASSDL ENMDPNL
Length:637
Mass (Da):69,758
Last modified:June 13, 2006 - v1
Checksum:i36CD14D1B5BB5558
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ489562 mRNA. Translation: ABF29535.1.
RefSeqiNP_001037718.1. NM_001044253.1.
UniGeneiRn.140285.

Genome annotation databases

EnsembliENSRNOT00000021265; ENSRNOP00000021265; ENSRNOG00000015878.
GeneIDi367645.
KEGGirno:367645.
UCSCiRGD:1586053. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ489562 mRNA. Translation: ABF29535.1.
RefSeqiNP_001037718.1. NM_001044253.1.
UniGeneiRn.140285.

3D structure databases

ProteinModelPortaliQ1HG60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021265.

Proteomic databases

PaxDbiQ1HG60.
PRIDEiQ1HG60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021265; ENSRNOP00000021265; ENSRNOG00000015878.
GeneIDi367645.
KEGGirno:367645.
UCSCiRGD:1586053. rat.

Organism-specific databases

CTDi80119.
RGDi1586053. Pif1.

Phylogenomic databases

eggNOGiKOG0987. Eukaryota.
COG0507. LUCA.
GeneTreeiENSGT00530000063561.
HOGENOMiHOG000132960.
HOVERGENiHBG106706.
InParanoidiQ1HG60.
KOiK15255.
OMAiRACTSGK.
OrthoDBiEOG091G03MY.
PhylomeDBiQ1HG60.

Miscellaneous databases

PROiQ1HG60.

Gene expression databases

BgeeiENSRNOG00000015878.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
HAMAPiMF_03176. PIF1. 1 hit.
InterProiIPR003840. DNA_helicase.
IPR010285. DNA_helicase_pif1-like.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23274. PTHR23274. 1 hit.
PfamiPF02689. Herpes_Helicase. 1 hit.
PF05970. PIF1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPIF1_RAT
AccessioniPrimary (citable) accession number: Q1HG60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: June 13, 2006
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.