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Protein

Endo-beta-1,4-glucanase B

Gene

eglB

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 4.0.1 Publication

Temperature dependencei

Optimum temperature is 52 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton donorBy similarity1
Active sitei262NucleophileBy similarity1

GO - Molecular functioni

  • cellulase activity Source: UniProtKB

GO - Biological processi

  • cellulose catabolic process Source: UniProtKB-KW
  • glucan catabolic process Source: UniProtKB

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH5 Glycoside Hydrolase Family 5
mycoCLAPiEGL5C_EMENI

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-beta-1,4-glucanase B (EC:3.2.1.4)
Short name:
Endoglucanase B
Alternative name(s):
Carboxymethylcellulase B
Cellulase B
Gene namesi
Name:eglB
ORF Names:AN5214
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000039405818 – 328Endo-beta-1,4-glucanase BAdd BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi95N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ1HFS8

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00003212

Structurei

3D structure databases

ProteinModelPortaliQ1HFS8
SMRiQ1HFS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000111120
InParanoidiQ1HFS8
KOiK01179
OrthoDBiEOG092C4BA5

Family and domain databases

InterProiView protein in InterPro
IPR001547 Glyco_hydro_5
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00150 Cellulase, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q1HFS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVNTLLVAV AAGTAMAAPQ LKKRAGFTFF GVTEAGAEFG EKSIPGVWGT
60 70 80 90 100
DYTFPDTESI LTLISKGFNT FRIPFLMERL TPEMTGSFDE GYLKNLTSVV
110 120 130 140 150
NAVTDAGAWA IVDAQNFGRF NGEIISSASD FQTWWKNVAA EFADNKNVIF
160 170 180 190 200
DTNNEFHDMD QTLVLDLNQA AINGIRAAGA TSQYIFVEGN SYTGAWTWTD
210 220 230 240 250
NNDNLKSLTD PQDKIVYEMH QYLDTDGSGT HETCVSETIG AERVESATQW
260 270 280 290 300
LKDNGKLGVI GEFAGGNNEI CRAAVKSLLD ALKENDDVWL GALWWAAGPW
310 320
WEDYMFSMEP TDGIAYTGML STLEAYMN
Length:328
Mass (Da):36,080
Last modified:June 13, 2006 - v1
Checksum:i7FBF3D7078476B4D
GO

Sequence cautioni

The sequence CBF81096 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence EAA62395 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490496 mRNA Translation: ABF50872.1
AACD01000089 Genomic DNA Translation: EAA62395.1 Sequence problems.
BN001305 Genomic DNA Translation: CBF81096.1 Sequence problems.
RefSeqiXP_662818.1, XM_657726.1

Genome annotation databases

EnsemblFungiiCADANIAT00003212; CADANIAP00003212; CADANIAG00003212
EAA62395; EAA62395; AN5214.2
GeneIDi2871506
KEGGiani:AN5214.2

Similar proteinsi

Entry informationi

Entry nameiEGLB_EMENI
AccessioniPrimary (citable) accession number: Q1HFS8
Secondary accession number(s): C8VF90, Q5B2L6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: June 13, 2006
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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