Q1H501 (RLMF_METFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Mfla_0165

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349921

Sequences

	Sequence		Length	Mass (Da)
	Q1H501 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 97AD7BB98D8570D9	FASTA	336	37,138
	10 20 30 40 50 60 MSQRSLPDEK SGLHPRNRHR QRYDFALLVE AHPPLAAFVR LNQYEDASID FADAQAVKTL 70 80 90 100 110 120 NQALLKCYYG IAAWDIPPQF LCPPIPGRAD YVHCLADLLA AGNDGNMPYG GNVRVLDIGT 130 140 150 160 170 180 GANLVYPIIG QKEYGWHFVG TDINVEALEN AQGIVAANPG LAGAIELRLQ RNVGAVFSGV 190 200 210 220 230 240 VQESERFDLT MCNPPFHASQ AAAMAGTERK WHHLERSRGK PTGKGVRRVR SGRMSGNRLN 250 260 270 280 290 300 FGGQAAELYC EGGEEGFISR MIKESAAVGA QCLWFTTLVS KAATLPAVYR SLRAVDAYKV 310 320 330 KTIDMAQGQK KSRFVAWTFH DTAGQRAWRR QHWRDV

References

[1]

"Complete sequence of Methylobacillus flagellatus KT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000284 Genomic DNA. Translation: ABE48436.1.
RefSeq	YP_544277.1. NC_007947.1.
3D structure databases
ProteinModelPortal	Q1H501.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1H501.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3999258.
GenomeReviews	Gene locus Mfla_0165 in contig CP000284_GR.
KEGG	mfa:Mfla_0165.
PATRIC	32265841. VBIMetFla97085_0168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	WSFVGSD.
PhylomeDB	Q1H501.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	MFLA265072:MFLA_0165-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_METFK

Accession

Primary (citable) accession number: Q1H501

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 27, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program