Q1H4R2 (HIS6_METFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazole glycerol phosphate synthase subunit HisF
EC=4.1.3.-

Alternative name(s):
IGP synthase cyclase subunit
IGP synthase subunit HisF
ImGP synthase subunit HisF
Short name=IGPS subunit HisF

Gene names

Name:	hisF
Ordered Locus Names:	Mfla_0254

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus › Methylobacillus flagellatum

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit By similarity. HAMAP-Rule MF_01013
Catalytic activity	5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. HAMAP-Rule MF_01013
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. HAMAP-Rule MF_01013
Subunit structure	Heterodimer of HisH and HisF By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	imidazoleglycerol-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Imidazole glycerol phosphate synthase subunit HisF HAMAP-Rule MF_01013		PRO_0000319460
Sites
	Active site	12	1	Potential
	Active site	131	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1H4R2 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 79163930FA6BF007	FASTA	252	27,056
	10 20 30 40 50 60 MSLAKRIIPC LDVTNGRVVK GVNFLELRDA GDPVEIARRY DEQGADELTF LDITASSDNR 70 80 90 100 110 120 GLMLGIIEQV ASQVFIPLTV GGGVREVEDI RRLLNAGADK VSINTAAVTN PQLVADAASR 130 140 150 160 170 180 FGSQCIVVAI DAKQVGDHWE IFTHGGRTPT GLNAVDWARK MVELGAGELL VTSMDRDGTK 190 200 210 220 230 240 MGFNLPLNRA ISEAVDVPII ASGGVGNLVH LVEGVREGGA DAVLAASIFH YGEYTVRQAK 250 EYMRDQGIEV RL

References

[1]

"Complete sequence of Methylobacillus flagellatus KT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000284 Genomic DNA. Translation: ABE48525.1.
RefSeq	YP_544366.1. NC_007947.1.
3D structure databases
ProteinModelPortal	Q1H4R2.
SMR	Q1H4R2. Positions 2-252.
ModBase	Search...
Protein-protein interaction databases
STRING	265072.Mfla_0254.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE48525; ABE48525; Mfla_0254.
GeneID	3999074.
KEGG	mfa:Mfla_0254.
PATRIC	32266023. VBIMetFla97085_0259.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0107.
HOGENOM	HOG000224612.
KO	K02500.
OMA	RVVKGTN.
ProtClustDB	CLSK2529135.
Enzyme and pathway databases
BioCyc	MFLA265072:GHWJ-317-MONOMER.
UniPathway	UPA00031; UER00010.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01013. HisF.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR004651. HisF. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00735. hisF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS6_METFK

Accession

Primary (citable) accession number: Q1H4R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 27, 2006
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program