Reviewed, UniProtKB/Swiss-Prot Q1H4H0 (HEM6_METFK)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, aerobic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	Mfla_0346

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: HAMAP protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000119807
Regions
	Region	46 – 55	10	Important for dimerization By similarity
	Region	106 – 108	3	Substrate binding By similarity
	Region	238 – 273	36	Important for dimerization By similarity
	Region	256 – 261	6	Substrate binding By similarity
Sites
	Active site	104	1	Proton donor By similarity
	Binding site	90	1	Substrate By similarity
	Site	173	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1H4H0-1 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: A08E2D00E40C2FAC	FASTA	302	34,871
	10 20 30 40 50 60 MNPTAIFDYL QGLQTRIVEA IELVDGKRFL HDSWQRAEGG GGTSCMLEEG NVFERAGIGF 70 80 90 100 110 120 SHVIGNKLPP SASVAHPEAA GRSWQAMGVS LVFHPRNPYV PTVHMNVRFF VAEKPGEEPI 130 140 150 160 170 180 WWFGGGMDLT PYYGFEEDAV HFHRTCRDAV APFGEQYYPR FKKECDDYFY LKHRKEARGI 190 200 210 220 230 240 GGIFFDDFNE LGFEQSFAFQ RSVGDAFINA YLPIVQRRKD IPYSERERDF QAYRRGRYVE 250 260 270 280 290 300 FNLIYDRGTI FGLQSNGRTE SILLSMPPIV KWRYDWKPEA GSPEARLYSD FITGRDWLTE HK

References

[1]

"Complete sequence of Methylobacillus flagellatus KT." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000284 Genomic DNA. Translation: ABE48617.1.
RefSeq	YP_544458.1.
3D structure databases
SMR	Q1H4H0. Positions 4-298.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1H4H0.
Genome annotation databases
GeneID	3999313.
GenomeReviews	Gene locus Mfla_0346 in contig CP000284_GR.
KEGG	mfa:Mfla_0346.
NMPDR	fig|265072.7.peg.345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	HMNTRFV.
PhylomeDB	Q1H4H0.
Enzyme and pathway databases
BioCyc	MFLA265072:MFLA_0346-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidas. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_METFK

Accession

Primary (citable) accession number: Q1H4H0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 27, 2006
Last modified:	January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)