Reviewed, UniProtKB/Swiss-Prot Q1H378 (DADA_METFK)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	Mfla_0791

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity. HAMAP MF_01202
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000066099
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q1H378-1 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: CF39A276952DE939	FASTA	417	45,859
	10 20 30 40 50 60 MKVIVLGSGV IGVTAAWYLA EAGHEVIVLD RQPGPALETS YANAGQVSPG YASPWAAPGI 70 80 90 100 110 120 PLKAIKWMLR RHAPLFIKPD GTWQQLRWMW QLLQNCTGTR YEQNKARMVR LAEYSRDCLD 130 140 150 160 170 180 QLRADTGIEY EGRQRGTLQL FRTREQLDAA LRDIEVLRFA HVPFELLPPS LLTKAEPALE 190 200 210 220 230 240 HVKHKLTGGL WLPRDETGDC RLFTVRLADM AAARGVQFRY GQEISHLAIE SGNVNGVVVS 250 260 270 280 290 300 GVRVQADAYV VALAAYSVDV LKGVLDLPVY PVKGYSITVP IIDEALAPVS TVLDETYKTA 310 320 330 340 350 360 VTRFDNRIRV GGMAELVGFD RRLSPRREGT LKLIVHDLFP GAADLSQTSF WTGLRPMTPD 370 380 390 400 410 GPPIIGATPI PNLYLNTGHG TLGWTMACGS GKLLADIISG KKTDIQHDDL GIGRYAK

References

[1]

"Complete sequence of Methylobacillus flagellatus KT." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000284 Genomic DNA. Translation: ABE49059.1.
RefSeq	YP_544900.1.
3D structure databases
SMR	Q1H378. Positions 1-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1H378.
Genome annotation databases
GeneID	4000224.
GenomeReviews	Gene locus Mfla_0791 in contig CP000284_GR.
KEGG	mfa:Mfla_0791.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0665.
HOGENOM	HBG729204.
OMA	MFQKHAP.
PhylomeDB	Q1H378.
Enzyme and pathway databases
BioCyc	MFLA265072:MFLA_0791-MONOMER.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_METFK

Accession

Primary (citable) accession number: Q1H378

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 27, 2006
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)