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Q1H2I8 (PUR4_METFK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL1
Ordered Locus Names:Mfla_0887
AND
Name:purL2
Ordered Locus Names:Mfla_1031
OrganismMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]
Taxonomic identifier265072 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length1293 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Sequence caution

The sequence ABE49155.1 differs from that shown. Reason: Erroneous initiation.

The sequence ABE49299.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12931293Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000264580

Regions

Domain1040 – 1293254Glutamine amidotransferase type-1
Nucleotide binding308 – 31912ATP Potential

Sites

Active site11331Nucleophile By similarity
Active site12581 By similarity
Active site12601 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1H2I8 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: ED2FEE4AC3F4EDB2

FASTA1,293140,059
        10         20         30         40         50         60 
MISLRGSAAL SPFRIEKILA ALKGSAPRIT HLYAEFWHFA WSDQPLSEAQ QEVLKQILTY 

        70         80         90        100        110        120 
GPRMSEEAPA GELFLVIPRP GTISPWSSRA TDIARHCGIE AIQRLERGIA FYAATADGSP 

       130        140        150        160        170        180 
LTDAEKAALR PLIHDRMTEA VFASLTDAQK LYHTAEPAPL STVDILSGGK AALEAANAEM 

       190        200        210        220        230        240 
GLALSPDEVD YLIENFQRMG RNPTDVELMM FAQANSEHCR HKIFNADWVI DGVAQAQSLF 

       250        260        270        280        290        300 
GMIRNTHKLN PGKTVVAYAD NASIVEGGKT KRFYPLADGQ YGFVEEDMHF LMKVETHNHP 

       310        320        330        340        350        360 
TAISPFAGAA TGAGGEIRDE GATGSGSKPK AGLTGFSVSN LHIPGFKQPW EHNNGKPDRI 

       370        380        390        400        410        420 
ASALQIMVDG PLGGAAYNNE FGRPNIAGYF RTLEIESAGE IRGYHKPIML AGGVGNISAR 

       430        440        450        460        470        480 
HAKKNPIPPG AALIQLGGPA MLIGLGGGAA SSMDTGANTE NLDFDSVQRG NPELERRAQE 

       490        500        510        520        530        540 
VIDRCWQLGD KNPILSIHDV GAGGISNAFP ELVNDAGVGA RFQLRDVHNE EPGMSPREIW 

       550        560        570        580        590        600 
SNEAQERYVM AVRKEDLPLF AEICERERCP FAVVGEATEE KRLVVSDRHF GNTPVDMDLS 

       610        620        630        640        650        660 
VLLGKPPKMT RDVQHVAREL PAFDHSRIDL KEAAQRVLRL PGVADKTFLI TIGDRSVTGM 

       670        680        690        700        710        720 
IARDQMVGPW QVPVADVAVT LDGFETYRGE AFAIGEKAPL ALIDAPASGR MAIGEAITNI 

       730        740        750        760        770        780 
AASLIEDIAD LKLSANWMAP AGHPGEDAAL FDTVKAVGME LCPQLGISIP VGKDSMSMKT 

       790        800        810        820        830        840 
VWEERNEKKA VTAPISLVVT AFAPTADARK TLTPQLRTDL GDTRLLLIDL GAGRNRLGGS 

       850        860        870        880        890        900 
ALAQVYGSVG NVAPDVEDAD SLKHFFNAVQ KLNREGRLLA YHDRSDGGLF ATVVEMAFAG 

       910        920        930        940        950        960 
RTGLELDIAS LGEDAVAVLY NEELGAVLQV RAADLDAITA ELETTLRGKV HVIGAPASHG 

       970        980        990       1000       1010       1020 
DIVIRQGTKL VFAESRVALH RAWSETTYQM QKLRDNPVCA QQEYDRLLDE RDAGLHAKLT 

      1030       1040       1050       1060       1070       1080 
FDINENIAAP YIASGARPKM AILREQGVNG QVEMAAAFDR AGFNAFDVHM SDIISGRVSL 

      1090       1100       1110       1120       1130       1140 
KDFAGFVACG GFSYGDVLGA GEGWAKSILF NPRARDEFTA FFNRTDSFAL GVCNGCQMMS 

      1150       1160       1170       1180       1190       1200 
NLHSIIPGAG HWPHFVRNRS EQFEARVAMV EVLDSPSLFF NGMAGSRMPI AVAHGEGYAE 

      1210       1220       1230       1240       1250       1260 
FADAAAQQRA QDARLVTLRY VDNSGLPTEV YPFNPNGSPQ GITGLTTADG RFSIMMPHPE 

      1270       1280       1290 
RVFRTVQHSW HPDGWGEDGP WIRMFRNARK FIG

« Hide

References

[1]"Complete sequence of Methylobacillus flagellatus KT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000284 Genomic DNA. Translation: ABE49299.1. Different initiation.
CP000284 Genomic DNA. Translation: ABE49155.1. Different initiation.
RefSeqYP_544996.1. NC_007947.1.
YP_545140.1. NC_007947.1.

3D structure databases

ProteinModelPortalQ1H2I8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ1H2I8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4000097.
4001038.
GenomeReviewsGene locus Mfla_0887 in contig CP000284_GR.
Gene locus Mfla_1031 in contig CP000284_GR.
KEGGmfa:Mfla_0887.
mfa:Mfla_1031.
PATRIC32267389. VBIMetFla97085_0932.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHBG335309.
PhylomeDBQ1H2I8.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycMFLA265072:MFLA_0887-MONOMER.
MFLA265072:MFLA_1031-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_METFK
AccessionPrimary (citable) accession number: Q1H2I8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: January 25, 2012
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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