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Q1H1K2 (GLGB_METFK) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Mfla_1367
OrganismMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]
Taxonomic identifier265072 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus

Protein attributes

Sequence length725 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7257251,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260666

Sites

Active site4061Nucleophile By similarity
Active site4591Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1H1K2 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 5ABCB11664174492

FASTA72583,702
        10         20         30         40         50         60 
MLTKSKQDPH LNHILNARHH DPFSYLGLHQ DGTQYILRLF QPFAARAWLQ TASGWVPLQR 

        70         80         90        100        110        120 
SHEAGLFEWK GNTPPPVPCR IRLEENGHSW ETYDAYTFWT TLSPEELYLF GEGRLKQAYR 

       130        140        150        160        170        180 
TFGAHLCEQQ GVAGVRFVVW APNAERVSVI GNFNHWDGRM HQMRVHGSSG VWEIFIPGLT 

       190        200        210        220        230        240 
SSDLYKFEIR NRQTGQILVK TDPYGFSFEQ RPGTAARVTA HGHYAWQDAE WLEDRARADW 

       250        260        270        280        290        300 
LHAPFNCYEV HLGSWRRDAH GEFLGYRELA HQLVPYMQEM GYTHIELLPV SEHPLNESWG 

       310        320        330        340        350        360 
YQTTGYFAPT NRFGSPDDLR YFVDQCHQAG IGVILDWVPG HFPKDDWALA RFDGSALFEH 

       370        380        390        400        410        420 
EDPRLGEHQD WGTYIFNYGR NEVRNFLLAS AHYWLEEFHM DGLRVDAVAS MLYLDYSRKE 

       430        440        450        460        470        480 
GEWLPNRHGG RENLEAIDFL KQLNVMVHED FPGALTIAEE STSWPMVSRP VYLGGLGFSM 

       490        500        510        520        530        540 
KWNMGWMNDT LSYMQHDPIH RRFHHDKLTF GQIYAYSENF VLPFSHDEVV HGKRSLLDKM 

       550        560        570        580        590        600 
PGDTWQKFAN LRLLATYQMT ASGKKLNFMG NELAQGREWN VNSSLDWHLL DLHWHQGIQR 

       610        620        630        640        650        660 
LHRDLSHLYR QVPALHELDF DAHGFEWIDC SDSDQSIINY LRRARDGSFV LVLLNFTPVL 

       670        680        690        700        710        720 
RSGYRVGTPL AGHYREIFNS DAGYYGGSNQ GNGNGLQTEA YPWMGHSHSL VVTIPPLGGV 


IIQPG 

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References

[1]"Complete sequence of Methylobacillus flagellatus KT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000284 Genomic DNA. Translation: ABE49635.1.
RefSeqYP_545476.1. NC_007947.1.

3D structure databases

ProteinModelPortalQ1H1K2.
SMRQ1H1K2. Positions 117-724.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265072.Mfla_1367.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE49635; ABE49635; Mfla_1367.
GeneID4000790.
KEGGmfa:Mfla_1367.
PATRIC32268445. VBIMetFla97085_1454.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMALSYMQHD.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycMFLA265072:GHWJ-1399-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_METFK
AccessionPrimary (citable) accession number: Q1H1K2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: June 27, 2006
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries