Q1H149 (LPXD_METFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Mfla_1520

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000264396
Sites
	Active site	240	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1H149 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 12EB16209C77764C	FASTA	350	37,051
	10 20 30 40 50 60 MNKSYSLREI VSTLGGELLG KDDVLISRVA SLANAQPGQI SFLTDSKYRS VLATTNASAV 70 80 90 100 110 120 ILTPQNRDIT ALPRILTDNP YAYFAKVSDL LNPKPEYVAG VDDTAVIAPS AQVPASCTIM 130 140 150 160 170 180 AKAVVGANVV LGEHVVVHPG CVIGEGVEIG AHSVLHANVT IYHHCMIGER CNIFSGSVIG 190 200 210 220 230 240 GDGFGYAPEE GRWVKIPQVG RVVIEHDVDI GANTTIDRGA IDDTIIHEGC KIDNLVQIGH 250 260 270 280 290 300 NCRIGAHSVI AGCVGIAGSA VLGKHCRIGG AAMILGHLEI ADGVTVSPGS MITRSLMKAG 310 320 330 340 350 TYTALMPFQS HDEWLRTAAG IRRLGELAER VKQLEKQLAP QQVSGIQRDK

References

[1]

"Complete sequence of Methylobacillus flagellatus KT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000284 Genomic DNA. Translation: ABE49788.1.
RefSeq	YP_545629.1. NC_007947.1.
3D structure databases
ProteinModelPortal	Q1H149.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1H149.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4000834.
GenomeReviews	Gene locus Mfla_1520 in contig CP000284_GR.
KEGG	mfa:Mfla_1520.
NMPDR	fig|265072.7.peg.1542.
PATRIC	32268773. VBIMetFla97085_1613.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	Q1H149.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	MFLA265072:MFLA_1520-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 5 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_METFK

Accession

Primary (citable) accession number: Q1H149

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	June 27, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program