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Q1H075 (NAGZ_METFK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:Mfla_1845
OrganismMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]
Taxonomic identifier265072 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_1000005656

Regions

Region168 – 1692Substrate binding By similarity

Sites

Active site1811Proton donor/acceptor By similarity
Active site2521Nucleophile By similarity
Binding site641Substrate By similarity
Binding site721Substrate By similarity
Binding site1381Substrate By similarity
Site1791Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1H075 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 8FFB5349D0C0003F

FASTA34938,596
        10         20         30         40         50         60 
MTLGPIMLDV VGTELTQEDI RRIRHPLVGG VILFARNFES PAQLKALTDS IHAVRQPPLL 

        70         80         90        100        110        120 
IAVDHEGGRV QRFREGFTRI PPMREFGKIW DEHPRKARQL AEEAGWIIAA ELRAHGIDFS 

       130        140        150        160        170        180 
FTPVLDMDYG ESQVIGDRAF HGDRQAINEL AFSLMQGLKR GGMAAVGKHF PGHGYVVADS 

       190        200        210        220        230        240 
HVAIPVDERD FDQIASTDMQ TFRQLIDDGI QAIMPAHVIY PKVDNKPAGF SERWLQKVLR 

       250        260        270        280        290        300 
QRLGFNGVIF SDDLSMEGAG VVGDVTQRAL AALNAGCDMV LLCNQPDKAD ELLANLKWDI 

       310        320        330        340 
QAQSLARLAR MHGGRHPSSM VALHEDPDFM HAVHRVGQVG KADGDLPFA 

« Hide

References

[1]"Complete sequence of Methylobacillus flagellatus KT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000284 Genomic DNA. Translation: ABE50112.1.
RefSeqYP_545953.1. NC_007947.1.

3D structure databases

ProteinModelPortalQ1H075.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265072.Mfla_1845.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE50112; ABE50112; Mfla_1845.
GeneID4000972.
KEGGmfa:Mfla_1845.
PATRIC32269459. VBIMetFla97085_1950.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMAAGFSSYW.
OrthoDBEOG6BCT06.
ProtClustDBPRK05337.

Enzyme and pathway databases

BioCycMFLA265072:GHWJ-1891-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAGZ_METFK
AccessionPrimary (citable) accession number: Q1H075
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 27, 2006
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries