Q1GZS1 (SYFA_METFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Mfla_1999

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus › Methylobacillus flagellatum

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP-Rule MF_00281
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281		PRO_1000006860
Sites
	Metal binding	253	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1GZS1 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 09607B9119D63EED	FASTA	327	36,981
	10 20 30 40 50 60 MQNLDHIISE AQQDFASCAS IADLEQAKAR YLGKSGSLTE ALKGLGKLSA EERPAAGAAI 70 80 90 100 110 120 NVVKQQVEAA LKQRRDAILQ AEQERQLASE TIDVTLPART RGTGGLHPVS LTLRRVEELF 130 140 150 160 170 180 RSIGFEVAEG PEIESDFYNF TALNIPEDHP ARAMHDTFYV DDKHVLRTHT SPVQVHYMQD 190 200 210 220 230 240 KAPPLKIIAP GRVYRVDSDA THSPMFHQVE GLWVDEHISF ANLKGVVQDF LQRFFERDDL 250 260 270 280 290 300 QVRFRPSFFP FTEPSAEMDM SWNGGWLEIG GCGMVHPEVF RHVGIDSEQY RGFAFGLGVE 310 320 RLTMLRYGVN DLRLFFENDL RFLKQFA

References

[1]

"Complete sequence of Methylobacillus flagellatus KT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000284 Genomic DNA. Translation: ABE50266.1.
RefSeq	YP_546107.1. NC_007947.1.
3D structure databases
ProteinModelPortal	Q1GZS1.
ModBase	Search...
Protein-protein interaction databases
STRING	265072.Mfla_1999.
Proteomic databases
PRIDE	Q1GZS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABE50266; ABE50266; Mfla_1999.
GeneID	4000810.
KEGG	mfa:Mfla_1999.
PATRIC	32269785. VBIMetFla97085_2111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HOG000242675.
KO	K01889.
OMA	LTHTPMF.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	MFLA265072:GHWJ-2048-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_ligase_II_N. IPR022911. Phe_tRNA_ligase_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_METFK

Accession

Primary (citable) accession number: Q1GZS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 27, 2006
Last modified:	May 29, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program