Q1GZR7 (SYT_METFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Mfla_2003

Organism

Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]

Taxonomic identifier

265072 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Methylophilales › Methylophilaceae › Methylobacillus

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 635	635	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000020428
Regions
	Region	242 – 533	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q1GZR7 [UniParc]. Last modified June 27, 2006. Version 1. Checksum: 93DAE3336932F9C1	FASTA	635	72,347
	10 20 30 40 50 60 MPVIRLPDGS ERKFDGPVTV AEVAMNIGAG LARAALGGKV NGKAVDTSYL ITDDADLSII 70 80 90 100 110 120 TDRDAEGLAI IRHSTAHLLA QAVKQLFPDA QVTIGPVIEN GFYYDFSYKR PFTPEDLQKI 130 140 150 160 170 180 EARMQELAKR DIPIVRTVME RDEAVAYFQS IGEQYKAEII GSIPADQEIS LYTQDDFTDL 190 200 210 220 230 240 CRGTHVPSTG KLKAFKLMKV AGAYWRGDSN NEMLQRIYGT AWAKKEDLDA YLHMLEEAEK 250 260 270 280 290 300 RDHRKLGRQL DFFHMQDEAP GMVFWHPRGW VIWQEVEQYM RNMFREFGYQ EVRTPTIMDR 310 320 330 340 350 360 VMWEKSGHWQ NYHDNMFTTA SENRDYAVKP MNCPGHIQIF NSTLHSYRDL PLRLAEFGSC 370 380 390 400 410 420 HRNEPSGALH GLMRVRGFTQ DDAHIFCTED QVKDEVADFI VMLYKAYKDF GFNEVLVKLS 430 440 450 460 470 480 TRPEKRVGTD EAWDKAEEAL AVALRQNNLD FELQPGEGAF YGPKIEFTLK DSLGRLWQCG 490 500 510 520 530 540 TIQLDFNLPE RLGAEYVDED NSRKHPVMLH RAIVGSMERF LGILIENYAG AMPAWLAPVQ 550 560 570 580 590 600 AMVLNISDGQ ADYVSSVVAE LRKNGFRVDS DLRNEKITYK IREHSLQKLP YLLIAGEREM 610 620 630 QTGQVAVRTR KGEDLGSMPL SAFIERLKSD VADKV

References

[1]

"Complete sequence of Methylobacillus flagellatus KT." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000284 Genomic DNA. Translation: ABE50270.1.
RefSeq	YP_546111.1. NC_007947.1.
3D structure databases
ProteinModelPortal	Q1GZR7.
SMR	Q1GZR7. Positions 2-634.
ModBase	Search...
Protein-protein interaction databases
STRING	Q1GZR7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4000814.
GenomeReviews	Gene locus Mfla_2003 in contig CP000284_GR.
KEGG	mfa:Mfla_2003.
NMPDR	fig|265072.7.peg.2029.
PATRIC	32269793. VBIMetFla97085_2115.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HBG352811.
OMA	MIIRNIL.
PhylomeDB	Q1GZR7.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	MFLA265072:MFLA_2003-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_METFK

Accession

Primary (citable) accession number: Q1GZR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 27, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program