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Q1GZB5 (SYL_METFK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Mfla_2155
OrganismMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]
Taxonomic identifier265072 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus

Protein attributes

Sequence length817 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 817817Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009372

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1GZB5 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: FE410E4C7D9E64DD

FASTA81791,608
        10         20         30         40         50         60 
MQQQYPFRDI EQQAQQYWES NATFTATEVS DKPKYYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VLSRFHRMRG YNVMQPMGWD AFGLPAENAA IKHNVAPAGW TYSNIEHMKG QLKSLGLAVD 

       130        140        150        160        170        180 
WQREIATCKP DYYRWEQWLF TELYKKGLIY KKTATVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVEKRDIPQ YFMKITAYAE ELLADLDKLD GWPEQVKTMQ RNWIGKSYGC EVEFPLAVGN 

       250        260        270        280        290        300 
GTLKVYTTRP DTLMGATYVA IAAEHALATQ AAKDNPALQA FIEECKRGSV AEADLATAEK 

       310        320        330        340        350        360 
KGMNSGLFVL HPITGEKLPV WVANYVLISY GEGAVMAVPA HDERDFEFAS KYKLPIKAVI 

       370        380        390        400        410        420 
KPAHGELELP LQAAYTEHGI LFDSGDFNGL DFSQASDAIA AALAAKNLGK RRTQYRLRDW 

       430        440        450        460        470        480 
GISRQRYWGC PIPIVHCPSC GEVPVPAEQL PVVLPEDVVM DGVGSPIKKD PAFYETTCPS 

       490        500        510        520        530        540 
CGEKATRETD TMDTFVESSW YFARYASFDA HSSMVDERAN YWLPVDQYIG GIEHAILHLL 

       550        560        570        580        590        600 
YARFFNKLMR DVGLVRHDEP FTKLLTQGMV LKDGSKMSKS KGNTVDPQEL IDNYGADTAR 

       610        620        630        640        650        660 
LFMMFAAPPE QSLEWSDAGV EGAHRFLKRV WTAVANHVES GIVAAFTMGD LSAELKAFRR 

       670        680        690        700        710        720 
QLHQTIEKVT DDYGRRHAFN TAIAAVMELM NAYAKIEGHD ATTRAIRQEA LENIILLLSP 

       730        740        750        760        770        780 
IVPHICHALW QALRPGTQLL DTRWPVANKA AMVQDEIELV LQVNGKLRGS MTVSRTLDKA 

       790        800        810 
AIEALAVQQE CIQKYLAEGS VRKIIVVPNK LVNIVVG 

« Hide

References

[1]"Complete sequence of Methylobacillus flagellatus KT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000284 Genomic DNA. Translation: ABE50422.1.
RefSeqYP_546263.1. NC_007947.1.

3D structure databases

ProteinModelPortalQ1GZB5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265072.Mfla_2155.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE50422; ABE50422; Mfla_2155.
GeneID3999711.
KEGGmfa:Mfla_2155.
PATRIC32270111. VBIMetFla97085_2266.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycMFLA265072:GHWJ-2212-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_METFK
AccessionPrimary (citable) accession number: Q1GZB5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 27, 2006
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries