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Q1GZA6

- BIOB_METFK

UniProt

Q1GZA6 - BIOB_METFK

Protein

Biotin synthase

Gene

bioB

Organism
Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (27 Jun 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi119 – 1191Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi150 – 1501Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi210 – 2101Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi282 – 2821Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMFLA265072:GHWJ-2221-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Mfla_2164
    OrganismiMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
    Taxonomic identifieri265072 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus
    ProteomesiUP000002440: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Biotin synthasePRO_0000381461Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi265072.Mfla_2164.

    Structurei

    3D structure databases

    ProteinModelPortaliQ1GZA6.
    SMRiQ1GZA6. Positions 28-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiCATEDEA.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q1GZA6-1 [UniParc]FASTAAdd to Basket

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    MRDSVIDIRG LDRVQRARVQ AKEEAPKRWS VDDIVALFEL PFSDLMHRAQ    50
    SVHRENFDPN GVQVSTLLSI KTGGCSEDCG YCPQAARYHT DVEKQDLMQL 100
    EEVLAAARAA KENGASRFCM GAAWRSPKQR DLEPVLAMIR EVKAMGLETC 150
    ATLGMLKDGQ AEQLKEAGLD YYNHNLDTAP EYYGEVITTR TYQDRLDTLD 200
    RVREQDINVC CGGIIGMGES RVQRAGLLAQ LANMERPPES VPINLLTQVE 250
    GTPMYGMDEL DPFEFVRTIA AARITMPQSF VRLSAGRQSM HEGIQALCFL 300
    AGANSIFYGE KLLTTGNPEA EADRKLFDKL GIHPL 335
    Length:335
    Mass (Da):37,315
    Last modified:June 27, 2006 - v1
    Checksum:i39658843A3268666
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000284 Genomic DNA. Translation: ABE50431.1.
    RefSeqiYP_546272.1. NC_007947.1.

    Genome annotation databases

    EnsemblBacteriaiABE50431; ABE50431; Mfla_2164.
    GeneIDi3999720.
    KEGGimfa:Mfla_2164.
    PATRICi32270129. VBIMetFla97085_2275.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000284 Genomic DNA. Translation: ABE50431.1 .
    RefSeqi YP_546272.1. NC_007947.1.

    3D structure databases

    ProteinModelPortali Q1GZA6.
    SMRi Q1GZA6. Positions 28-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 265072.Mfla_2164.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABE50431 ; ABE50431 ; Mfla_2164 .
    GeneIDi 3999720.
    KEGGi mfa:Mfla_2164.
    PATRICi 32270129. VBIMetFla97085_2275.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi CATEDEA.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci MFLA265072:GHWJ-2221-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KT / ATCC 51484 / DSM 6875.

    Entry informationi

    Entry nameiBIOB_METFK
    AccessioniPrimary (citable) accession number: Q1GZA6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: June 27, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3