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Q1GZA6 (BIOB_METFK) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Mfla_2164
OrganismMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]
Taxonomic identifier265072 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Biotin synthase HAMAP-Rule MF_01694
PRO_0000381461

Sites

Metal binding751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1191Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1501Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2101Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2821Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1GZA6 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 39658843A3268666

FASTA33537,315
        10         20         30         40         50         60 
MRDSVIDIRG LDRVQRARVQ AKEEAPKRWS VDDIVALFEL PFSDLMHRAQ SVHRENFDPN 

        70         80         90        100        110        120 
GVQVSTLLSI KTGGCSEDCG YCPQAARYHT DVEKQDLMQL EEVLAAARAA KENGASRFCM 

       130        140        150        160        170        180 
GAAWRSPKQR DLEPVLAMIR EVKAMGLETC ATLGMLKDGQ AEQLKEAGLD YYNHNLDTAP 

       190        200        210        220        230        240 
EYYGEVITTR TYQDRLDTLD RVREQDINVC CGGIIGMGES RVQRAGLLAQ LANMERPPES 

       250        260        270        280        290        300 
VPINLLTQVE GTPMYGMDEL DPFEFVRTIA AARITMPQSF VRLSAGRQSM HEGIQALCFL 

       310        320        330 
AGANSIFYGE KLLTTGNPEA EADRKLFDKL GIHPL 

« Hide

References

[1]"Complete sequence of Methylobacillus flagellatus KT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000284 Genomic DNA. Translation: ABE50431.1.
RefSeqYP_546272.1. NC_007947.1.

3D structure databases

ProteinModelPortalQ1GZA6.
SMRQ1GZA6. Positions 28-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265072.Mfla_2164.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE50431; ABE50431; Mfla_2164.
GeneID3999720.
KEGGmfa:Mfla_2164.
PATRIC32270129. VBIMetFla97085_2275.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAFADYEVL.
OrthoDBEOG622PMP.
ProtClustDBCLSK2309947.

Enzyme and pathway databases

BioCycMFLA265072:GHWJ-2221-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_METFK
AccessionPrimary (citable) accession number: Q1GZA6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 27, 2006
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways