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Q1GZA6

- BIOB_METFK

UniProt

Q1GZA6 - BIOB_METFK

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Protein

Biotin synthase

Gene

bioB

Organism
Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi119 – 1191Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi150 – 1501Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi210 – 2101Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi282 – 2821Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMFLA265072:GHWJ-2221-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Mfla_2164
OrganismiMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)
Taxonomic identifieri265072 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus
ProteomesiUP000002440: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Biotin synthasePRO_0000381461Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi265072.Mfla_2164.

Structurei

3D structure databases

ProteinModelPortaliQ1GZA6.
SMRiQ1GZA6. Positions 28-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiCATEDEA.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q1GZA6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRDSVIDIRG LDRVQRARVQ AKEEAPKRWS VDDIVALFEL PFSDLMHRAQ
60 70 80 90 100
SVHRENFDPN GVQVSTLLSI KTGGCSEDCG YCPQAARYHT DVEKQDLMQL
110 120 130 140 150
EEVLAAARAA KENGASRFCM GAAWRSPKQR DLEPVLAMIR EVKAMGLETC
160 170 180 190 200
ATLGMLKDGQ AEQLKEAGLD YYNHNLDTAP EYYGEVITTR TYQDRLDTLD
210 220 230 240 250
RVREQDINVC CGGIIGMGES RVQRAGLLAQ LANMERPPES VPINLLTQVE
260 270 280 290 300
GTPMYGMDEL DPFEFVRTIA AARITMPQSF VRLSAGRQSM HEGIQALCFL
310 320 330
AGANSIFYGE KLLTTGNPEA EADRKLFDKL GIHPL
Length:335
Mass (Da):37,315
Last modified:June 27, 2006 - v1
Checksum:i39658843A3268666
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000284 Genomic DNA. Translation: ABE50431.1.
RefSeqiWP_011480385.1. NC_007947.1.
YP_546272.1. NC_007947.1.

Genome annotation databases

EnsemblBacteriaiABE50431; ABE50431; Mfla_2164.
GeneIDi3999720.
KEGGimfa:Mfla_2164.
PATRICi32270129. VBIMetFla97085_2275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000284 Genomic DNA. Translation: ABE50431.1 .
RefSeqi WP_011480385.1. NC_007947.1.
YP_546272.1. NC_007947.1.

3D structure databases

ProteinModelPortali Q1GZA6.
SMRi Q1GZA6. Positions 28-334.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 265072.Mfla_2164.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABE50431 ; ABE50431 ; Mfla_2164 .
GeneIDi 3999720.
KEGGi mfa:Mfla_2164.
PATRICi 32270129. VBIMetFla97085_2275.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi CATEDEA.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MFLA265072:GHWJ-2221-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT / ATCC 51484 / DSM 6875.

Entry informationi

Entry nameiBIOB_METFK
AccessioniPrimary (citable) accession number: Q1GZA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 27, 2006
Last modified: November 26, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3