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Q1GZ04 (DDL_METFK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Mfla_2266
OrganismMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]
Taxonomic identifier265072 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000057324

Regions

Domain102 – 297196ATP-grasp
Nucleotide binding128 – 18356ATP By similarity

Sites

Metal binding2511Magnesium or manganese 1 By similarity
Metal binding2641Magnesium or manganese 1 By similarity
Metal binding2641Magnesium or manganese 2 By similarity
Metal binding2661Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1GZ04 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 3D899A307512DA0F

FASTA30232,121
        10         20         30         40         50         60 
MRRDFGKVAV LLGGKSGERE VSLKSGAAVL AALRRNGVDA HAFDPAERAL SELAGFDRVF 

        70         80         90        100        110        120 
IALHGRYGED GTIQGALELM GIPYTGSGVM ASSVGMDKWR TKLLWRAAGV VTPDFELVTA 

       130        140        150        160        170        180 
DSNFDAIERK LGLPLFVKPA NEGSSIGISK VKAAGGLQAA YALAAKSDPL VIAEQFVGGG 

       190        200        210        220        230        240 
EYTVGILGGA ALPVIRIVPA NEFYDYEAKY LRDDTQYLCP CGLDASLEEQ IKAEALQAFR 

       250        260        270        280        290        300 
IVGGRGWGRV DFLMDEAGRH YFLEVNTSPG MTDHSLVPMG AKAVGLGFDE LVLSILELAH 


VG 

« Hide

References

[1]"Complete sequence of Methylobacillus flagellatus KT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000284 Genomic DNA. Translation: ABE50533.1.
RefSeqYP_546374.1. NC_007947.1.

3D structure databases

ProteinModelPortalQ1GZ04.
SMRQ1GZ04. Positions 6-300.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265072.Mfla_2266.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE50533; ABE50533; Mfla_2266.
GeneID4001360.
KEGGmfa:Mfla_2266.
PATRIC32270339. VBIMetFla97085_2380.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMACSGYSRV.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycMFLA265072:GHWJ-2323-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_METFK
AccessionPrimary (citable) accession number: Q1GZ04
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 27, 2006
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways