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Q1GYR6 (RUVC_METFK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Mfla_2354
OrganismMethylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) [Complete proteome] [HAMAP]
Taxonomic identifier265072 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaMethylophilalesMethylophilaceaeMethylobacillus

Protein attributes

Sequence length180 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 180180Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000332427

Sites

Metal binding91Magnesium By similarity
Metal binding741Magnesium By similarity
Metal binding1461Magnesium By similarity
Metal binding1491Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q1GYR6 [UniParc].

Last modified June 27, 2006. Version 1.
Checksum: 76041F822A09BC67

FASTA18018,958
        10         20         30         40         50         60 
MTFRILGIDP GLRLTGFGVI EKTGEKLAYV ASGTIKTTTG KGSEIEDLPT RLKIILDSLA 

        70         80         90        100        110        120 
EVIDNYQPQQ AAIEKVFVNV NPQSTLLLGQ ARGAAISALV LQGLPIAEYT ALQVKQSVVG 

       130        140        150        160        170        180 
HGHAAKDQVQ EMVKRLLNLP GLPRPDSADA LACAICHAHG GQGLGKLATA GFRVKGGRLI 

« Hide

References

[1]"Complete sequence of Methylobacillus flagellatus KT."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT / ATCC 51484 / DSM 6875.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000284 Genomic DNA. Translation: ABE50621.1.
RefSeqYP_546462.1. NC_007947.1.

3D structure databases

ProteinModelPortalQ1GYR6.
SMRQ1GYR6. Positions 5-163.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING265072.Mfla_2354.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABE50621; ABE50621; Mfla_2354.
GeneID4001450.
KEGGmfa:Mfla_2354.
PATRIC32270523. VBIMetFla97085_2471.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAPDCAAIE.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycMFLA265072:GHWJ-2413-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_METFK
AccessionPrimary (citable) accession number: Q1GYR6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 27, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families